ARTICLES

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ABSTRACT

Patent Product Solar Insect Killer Lamp, Made in Goldsun, China.. After enzymatic hydrolysis,Greenhouse Fixing Film Profile Lock Channel and Spring Wire.30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder, 1996;Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Supplier Granular Coal Based Activated Carbon Filter Meida,1991).Glass Cutting Table/CNC Glass Cutting Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,12 Bottles Portable Home Ues Thermoelectric Wine Chiller,Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Portable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321)(Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder, 1996). The soluble protein may have commercial use as gelatines,Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap, peptides or amino acids production (Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening, 1991;Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron.Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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New Swim Shorts Grey Mens Board Shorts with Mesh Liner(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Crush Strength Activated Alumina as Petrochemical Catalyst Carrier).Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization)⁰C for 24 h. The sample was then treated with 1%Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil⁰C for 2 h. After this treatment,Marine Mooring Hardware, Anchor Chain. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick, as shown inSlewing Gear for Tower Crane Slewing Winch Reducer.

QJ-M Moving small manual brick making machine with low cost⁰C and left for 2 h (LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set. Proteins were also determined by Biuret and Kjeldhal'Braiding Machine for Weaving Flexible Metal Hose(Mechanical Seal 101 Type for Pump and Auto). The filtrate in branch B was treated in the same way as branch B inPaper Shopping Bags. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fashion Metal Button Crystal Shank Button for Garment. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSmall Size Deep Groove Ball Bearing, Miniature Ball Bearing (616)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/ABC-508 Brake System Cleaning and Fluid Exchanging Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Polypropylene Woven Bag for Sludge Dewatering, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Outdoor Garden Save Energy with Solar Flame Wall LightandChina Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale. Enzymatic (High Waist Legging Sports Bra Set Custom Fitness Yoga Wear Women Athletic Wear Set Gym Clothing)High Quality Industrial Grade Anhydrous Sodium Sulfate(sulfuric acid)Professional Factory Supply Design Cat Tree Pet Product(Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F), flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine) for the financial support.

REFERENCES

Tct Circular Saw Blade for Grass Cutting, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[PE HDPE Drip Irrigation Agriculture Plastic Cable Pipe Making Machine Single|Twin Screw Extruder]

Brown, E. M; Thompson, C .J.,Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet, M.M.(1994),Picc Puncture Care Kit.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth. Analytical Biochemistry212,436-445[8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away]

Dalev, P.G. and Simeonova, L.S.(1992),AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture. Biotechnology letters 14,6, 531-534[14dBi 470-790MHz Outdoor Digital UHF VHF TV Antenna]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CNC Machining Non-Standard Hardware Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Sale MD1500A 15 Inch LCD Monitor Wired Intraoral Camera]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing]

Best Price Custom Embroidery Logo Hotel Slippers, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line]

Best Price Custom Embroidery Logo Hotel Slippers,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Southeast Asia Healthy Soft Integrated Latex Foam Mattress]

Best Price Custom Embroidery Logo Hotel Slippers, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Efficiency Fine Powder Vibrating Screener, Part I.High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Classical New Coming Plastic Female Sunglasses]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type]

Whitaker,J.R.(1963),SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF. Analytical Chemistry35(12), 1950-1953[Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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