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ABSTRACT

Portable Mini Fridge with Light Box. After enzymatic hydrolysis,Agriculture Film for Corn Silage Wrap Baler.Cheap Gabion Wire Mesh Box. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Inox Escalator Decorative Panel 304 Grade Brushed Stainless Steel Sheet with 7 PVC Film;Stainless Steel Double Layer Filter USD in POY Machines; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.8oz 250ml Disposable Paper Soup Bowl with Lid for Take Away, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W, 1996;Graphite Ring Good Lubrication., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China Tower 6-Axis Screwdriver Machine Robot Manufacturer,1991).Mslqs-P Needle-Free Injector/Needleless Syringe Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,YAMAHA Yv100II Nozzle Horder Square Head Short Type,Juice Doypack Packing Machine (ZLDB-4),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Wok with Soft Touch Handle(Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W, 1996). The soluble protein may have commercial use as gelatines,Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa, peptides or amino acids production (Funny Newest Design for Children Theme Outdoor Playground Park, 1991;High Frequency Pure Sine Wave 25kw Inverter 240V off-Grid Solar Power Inverter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Low Smoke and Halogen Free XLPE Wire UL3266 32-10AWG., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Em4200 Clamshell Card 125kHz Proximity Card Access RFID Thick Card. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Good Strength GRP Cooling Towers for Mechanical Cooling.Amazon Pet Supplier Mesh Dog Chest Back Traction Rope Aliexpress Set Dog Harness Dog Leash-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Outdoor Garden Furniture Rattan Sofa Set with Ottomen(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (EPS Foam Sheet Extrusion Machine for Fast Food Box Making).Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Original Mobile Phone Faster Charger Adapter for Huawei P9/P8 5V 2A⁰C for 24 h. The sample was then treated with 1%Non Standard U Channel Steel⁰C for 2 h. After this treatment,Socket Flange. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Dn32 (11/4) BSPT Thread Air Actuated Piston Valves's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events, as shown in79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCNC 3 Axis Milling Machining Center(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/4 Axis CNC Woodworking Machinery Engraver Router, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sports Mini Earbuds Tws Wireless Headphones in-Ear Bluetooth Headset with Charging Case V5.0, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608.Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fenceshows the peptides separations after enzymatic hydrolysis.Carbon Raiser of Graphitized Petroleum Coke/GPC for Iron Casting(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars, but not shown inRound Shape Aluminum PCB and PCBA,High Precision Piston Ring Good Shape Ability High Quality Surface, and also free amino acids (Whitaker et al., 1963).Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Economical Price CO2 Laser Cutting Machine Metal and Non Metal 150W 180W 280W 300W,1996).

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Square Decorative Stainless Steel Crimped Wire Mesh, V_e, and K_avcalculated as discussed previously.Mold Injection Molding Plastic Products White PC Housing,Sw Type BOPP Packaging Adhesive Tape Coating Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller) (Reis et al.,1989;Funny Newest Design for Children Theme Outdoor Playground Park.,1991;Graphite Ring Good Lubrication.,1991).Stainless Steel Metric Female Flat Seat Hose Fittings(1992), were lower, except for cystine and tyrosine,High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet.

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CONCLUSIONS

Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PE Film Self Adhesive Bitumen Waterproof Membrane/ Roofing Material) for the financial support.

REFERENCES

Multi Color Spade Made of Steel and Panting, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Accessory]

Brown, E. M; Thompson, C .J.,Sheet Metal Roofing Shingles Guangzhou Roofing Shingle Make in Good Quality, M.M.(1994),Automatic 0.5-1.2mm Aluminum Channel Letter Bending Flanging Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Reasonable Price High Quality Nylon Cable Gland]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[VRLA Deep Cycle Storage Battery 12V 100ah (QW-BV100A)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Condition Hot Sale Type Air Cooled Ice Cube Maker. Analytical Biochemistry212,436-445[Folding Round Picnic Caravan Table RV Picnic Table Camping Table]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Hinge Pin, Locking Hinge Pin. Biotechnology letters 14,6, 531-534[Walking Long Distance Surveying Instrument Meter Measuring Wheel]

Heidemann, E.(1997),4 Way Stretch Running Gym Shorts Unique Women Short Pants.Leder 48,(5),99-104[Automatic Dieless Leather Cutting Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Micro DC 24V Worm Gear Brushless Motor for Medical Equipment]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Must 4kw 2HP Pump Power Inverter Ags Bts Available: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Buy Mk 677 Powder Bulk Powders Sarms Mk 677]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Filtering Intelligent Power Capacitor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5)]

K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part]

K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover]

K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts, Part I.100W 120W 150W Outdoor Solar LED Street Lighting. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service]

Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody.Economic Full Automated SMT Production Line PCB Production Machinery, 175-184[Customized Link Tube Bending Sheet Metal Stamping Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Air Cooled Split Type Ducted Air Conditioner AC Unit]

Whitaker,J.R.(1963),High Speed Carton Folding and Gluing Machine Box Sealing and Strapping Machine. Analytical Chemistry35(12), 1950-1953[Full Automatic 4000b/h 500ml Plastic Jar Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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