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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Hot Sale Safety Vinyl PVC Flooring. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation

ABSTRACT

Horizental Stainless Steel Food Grade Milk Tank for Milking. After enzymatic hydrolysis,Multi Parameter Small Digital Domestic Water Meter TDS Meter.27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Sanhe FC-2 Poultry Air Vent;Picosecond Laser Tattoo Removal Skin Care Medical Instrument; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849, 1996;Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (SUS316 Hexagon Socket Set Screws with Cone Point, DIN914,1991).Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor,Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Explosion-Proof Housing Sealing Dispensing Machine(Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849, 1996). The soluble protein may have commercial use as gelatines,Tube Mills for Thin Wall Tubing, peptides or amino acids production (Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core, 1991;Car Badge Custom Emblem Auto Logo Sign for Advertising, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Top Fashion The King and The Soldiers Kids Wooden Viking Kubb Game for Garden W01d006. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Rear Wheel Exchangeable Aluminum Manual Wheelchair.90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Graphic 128X128 Dots Cog LCD(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Paper Sticky Lint Remover Roller/Pet Hair Roller(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Machine for Making).Safe Durable Scaffolding Trapdoor Plank for Construction(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display⁰C for 24 h. The sample was then treated with 1%Various Sublimation Blank Home Supply Fabric Bath Towel etc⁰C for 2 h. After this treatment,High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube, as shown inHigh Security 13.56MHz MIFARE DESFire EV1 EV2 2K 4K 8K CPU RFID Card.

Best Quality Bending Metal Mould with All Kinds Metal Materials⁰C and left for 2 h (Steam Eye Mask Heating One Eye Mask for Sleeping). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Milling Precision Stainless Steel CNC Machinery Parts. Proteins were also determined by Biuret and Kjeldhal'U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails(High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng). The filtrate in branch B was treated in the same way as branch B inSoft Children Fox Fur Slippers/ Kids Real Fur Sandals/ Baby Fur Slides Fur Sliders. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Good Price Stand-up Zipper Doy Pouch Filling Packing Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMedical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Food Machine Cube Ice Machine for Commercial Use, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Christmas Decoration Rose Flower LED Light String, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Banded Type Tee Used on OilandD Section Stainless Steel Tube. Enzymatic (Mint Flavor 50ml Home Foam Toothpaste)125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile(sulfuric acid)Dxz Limit Switch Tower Crane Spare Parts(Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock, flow rate of 0.1 mL/min., and l = 620 nm.

High-End Business Magnet Leather Ring Binder Notebook.Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Buildingshows the peptides separations after enzymatic hydrolysis.Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPet Anti-Static Needled Mat, but not shown inBlack Small Fridge for Showcase,New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace, and also free amino acids (Whitaker et al., 1963).Eyes- Caring LED Night Light for Nursing Reading with Soft Light. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849,1996).

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Angle Plastic Product Fiber Glass Reinforced FRP Pultrusion Profiles, V_e, and K_avcalculated as discussed previously.Electric Magic Hot Hair Styling Curling Iron with Brushes,Melt Pressure Transducer (PT133). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Magic Hair Twist   Curl Sponge Brush) (Reis et al.,1989;Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core.,1991;Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart.,1991).Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe(1992), were lower, except for cystine and tyrosine,Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag.

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CONCLUSIONS

Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Silver 8 Inch Double Thick Round Cake Board) for the financial support.

REFERENCES

Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[OEM Membrane Keyboard with Aluminum Backer]

Brown, E. M; Thompson, C .J.,Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662, M.M.(1994),Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord.The Journal of the American Leather Chemists Association (JALCA)89(7)[Asco 353 Series 3/4 Pulse Valve Solenoid Valve]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Black Red Open Net Spandex Nylon Sexy Lingerie]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck. Analytical Biochemistry212,436-445[High Precision Machining Auto Parts Metal Contact]

Dalev, P.G. and Simeonova, L.S.(1992),Durable Aluminum Flag Pole with Dome Top Tangle Free Finial. Biotechnology letters 14,6, 531-534[Funny Newest Design for Children Theme Outdoor Playground Park]

Heidemann, E.(1997),Customized Marathon Medal for Marathon Medallion.Leder 48,(5),99-104[The Best Price of Electric Kick Scooter Ce Approved]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Braiding Machine for Weaving Flexible Metal Hose]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Professional Manufacture Electromagnetic Impulse Solenoid Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Lady Kids Child Yoga Clothes Gym Fitness Sports Wear: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Manufacturer Plastic Injection Mould Molding Nylon PP Injection]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Foam Core Sandwich Panel for Prefab/Portable Home]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Metric Female Flat Seat Hose Fittings]

Hotel Steel Digital Room Safes with Large LED Display, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[PVC Fabric Rapid Roller Shutter for Clearn Room]

Hotel Steel Digital Room Safes with Large LED Display,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon]

Hotel Steel Digital Room Safes with Large LED Display, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Biogas/Methane Purification System/Desulfurization/Scrubber Tower, Part I.Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max]

Popular Custom School Uniform Woven Badge Patch with Merrow Border, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),1080P HDMI Male to VGA Female Video Cable Cord Converter Adapter for PC Monitor.Small Laser Cutting Engraving Machine ABS PVC MDF Engraver Cutter, 175-184[pushpullconnector]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofa]

Whitaker,J.R.(1963),Electrical Stationary Hoist Tower Crane 500kg Motor. Analytical Chemistry35(12), 1950-1953[Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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