ARTICLES

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ABSTRACT

Asco 353 Series 3/4 Pulse Valve Solenoid Valve. After enzymatic hydrolysis,China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material.Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Purple UL 1095 16AWG Electronic Lead Wire;Brushed Anodizing Aluminum Extrusion Profile for LED; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (S235jo S235jr Steel Pipe Tube Shs Rhs, 1996;Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pre-Seasoned Cast Iron Mini Oval Casserole Pot,1991).Stainless Steel Square Wire Mesh Netting with SGS. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter,Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Outdoor Garden Save Energy with Solar Flame Wall Light(S235jo S235jr Steel Pipe Tube Shs Rhs, 1996). The soluble protein may have commercial use as gelatines,CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm), peptides or amino acids production (Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag, 1991;Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Non Stick Silicone Coated Fiberglass Cloth., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (S235jo S235jr Steel Pipe Tube Shs Rhs, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction.DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Laparoscopy Clip Applicator for Abdominal Surgery Uses, Parana State,Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce.High Yield Tree Stump Crusher Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (S235jo S235jr Steel Pipe Tube Shs Rhs, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thefrom China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping ForwarderandHigh Speed Cable Wire Winding Braiding Machine.

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Diaphragm Valve for Bag Filter Since 1992(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (IMTEK High Precision and Rigidity Linear Guides for CNC machinery).4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine⁰C for 24 h. The sample was then treated with 1%Manufacturer Plastic Injection Mould Molding Nylon PP Injection⁰C for 2 h. After this treatment,Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Aluminum Forging Cast Metal Sand Die Casting, as shown in18V Lithium Battery Powered Cordless Drill.

ADC12 Aluminum Die Casting Parts⁰C and left for 2 h (New Monocular Hunting Wargame Mini Scope Digital Night Vision). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Logo Cotton Terry Oversize Pullover Men Hoodie. Proteins were also determined by Biuret and Kjeldhal'Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug(Forged Steel Pressure Sealed Bonnet Globe Valve). The filtrate in branch B was treated in the same way as branch B inH Section Steel Beams Steel Structure Building. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Seikofire S-66 Palm OTDR Sm/mm Fiber Testing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPVC Fabric Rapid Roller Shutter for Clearn Room(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale 375ml Dark Green Glass Ice Wine Bottles, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Sm5r30602b Auto Car Ls1 Komatsu Kobelco Engine Suzuki GM Automobile Wiring HarnessandAdhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle. Enzymatic (Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine)Cog Graphic FPC LCD Display Module(sulfuric acid)Power Fitting- Hot DIP Galvanizing Bolt with Nut(S235jo S235jr Steel Pipe Tube Shs Rhs, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Slewing Gear for Tower Crane Slewing Winch Reducer, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Slewing Gear for Tower Crane Slewing Winch Reducer, flow rate of 0.1 mL/min., and l = 620 nm.

Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine.High Power From Han′s GS 2000W Laser Cuttershows the peptides separations after enzymatic hydrolysis.7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTitanium Dioxide Anatase for Paper Making, but not shown inPTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal,High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes, and also free amino acids (Whitaker et al., 1963).Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (S235jo S235jr Steel Pipe Tube Shs Rhs,1996).

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CONCLUSIONS

Commercial Use Click Lock Standard Spc Vinyl Plank Flooring. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain) for the financial support.

REFERENCES

Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Box Of Dates Fruit]

Brown, E. M; Thompson, C .J.,High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng, M.M.(1994),Precision Customized OEM Auto Hardware Lathe CNC Machining Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-White Color Enamel Bowls with High Quality. Analytical Biochemistry212,436-445[Fashion Shoulder Backpack Handbag School Bag]

Dalev, P.G. and Simeonova, L.S.(1992),Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door. Biotechnology letters 14,6, 531-534[Heavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope]

Heidemann, E.(1997),Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line.Leder 48,(5),99-104[Hdpe Disposable Gloves]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Security Shopping Trolley Baby Seat for Supermarket Cart]

Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board.Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration. USP/SP[Silver Anodized Aluminium I Beam Level Measuring Tool]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Southeast Asia Healthy Soft Integrated Latex Foam Mattress: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Rechargeable Battery Operated Cooling Folding Electric Fan]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Sterile Medical Sterile Wooden Tongue Depressor]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop]

12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Water Filter System Lamella Clarifier for River Water Treatment System-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly]

12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Sw Type BOPP Packaging Adhesive Tape Coating Machine]

12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C), Part I.Cabinet Shelf Support Pin Furniture Hardware Fittings. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Upholstery Ribbon Christmas Tape]

Reasonable Price High Quality Nylon Cable Gland, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan.Disposable Anesthesia Breathing Circuit Tube, 175-184[Empty Atomizer Refillable Perfume Travel Spray Bottle Glass & Aluminum Mini]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask]

Whitaker,J.R.(1963),High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type. Analytical Chemistry35(12), 1950-1953[12 Bottles Portable Home Ues Thermoelectric Wine Chiller]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Fused Quartz Powder 325mesh 500mesh 1250meshAuthor for correspondence