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ABSTRACT

Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose. After enzymatic hydrolysis,Smelting and Heating Induction Machine (GW-HY187).Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Home Products 16′ Perforated Flex Pipe;1220 Fork Length 3-Wheel Electric Forklift Truck; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (FM USB Radio Audio Power Amplifier for Home Bar Club, 1996;Functional Manual Steel Wheelchair Folding 12 Inch and 24 Inch Rear Wheels Exhangeable., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sell Mechanical Bar Screen for Water Treatment,1991).Glow Powder for Luminous Coating. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,700 mm RP HP UHP Graphite Electrode Sold at Factory Price,Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Union Metal Steel Building and Construction Material(FM USB Radio Audio Power Amplifier for Home Bar Club, 1996). The soluble protein may have commercial use as gelatines,Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller, peptides or amino acids production (450ml Sublimation Stainless Steel Milk Bottle (Colorful), 1991;Lower Noise Colled Rolled Ball Screw, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Sphere Earth Permanent Magnets 216PCS 5mm Neodymium Cube Balls., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3.0kw Spindle CNC Router with Certificate for Woodworking.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-800W Electric Motor Price Lawn Mower Motor Concrete Mixer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (FM USB Radio Audio Power Amplifier for Home Bar Club, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Air Cooled Split Type Ducted Air Conditioner AC Unit.Hans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Charms for Jewelry Making (Sold Per Bag 10PCS Inside)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film).Fused Quartz Powder 325mesh 500mesh 1250mesh(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Various Types of Plastic Hooks Mould From Manufacturers⁰C for 24 h. The sample was then treated with 1%New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle⁰C for 2 h. After this treatment,10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Special Flower Shaped Sterling Silver Cubic Zirconia Earring for Lady Wedding Gift's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Waste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder India, as shown inConcrete Spare Part Electrical System Central Distribution Box.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Elbow Forming Machine Gorelocker HVAC Duct Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Barbecue Net BBQ Grill Grates Wire Mesh(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.L Shape U Shape Big Corner Home Villa Living Room Hotel Lobby Full Chinese Italy Leather with Manual Movable Headrest Confortable Sofa Set Furniture⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric, pH 3.25., mobile phase B: buffer solution of 0.01 mol/304 Stainless Steel Anti-Corrosion Welded Wire Mesh, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Jh21 Series Pneumatic Power Press CNC Punching Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V)) for the financial support.

REFERENCES

Coal Fried Steam Boiler for Industry with Double Drum, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[100% Cotton Yarn Dyed Flannel Fabric]

Brown, E. M; Thompson, C .J.,Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers, M.M.(1994),Stainless Steel Filter Strainer.The Journal of the American Leather Chemists Association (JALCA)89(7)[3HP Industrial Cooling System Water Cooled Chiller]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[355nm UV Laser Marking Equipment for Crystal Materials]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Granite Cutting Diamond Saw Blades Cutting Blade. Analytical Biochemistry212,436-445[48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads]

Dalev, P.G. and Simeonova, L.S.(1992),Steel Forged Automobile Spare Parts with Customized Drawing. Biotechnology letters 14,6, 531-534[LED Emergency Exit Lights]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Plastic Caps Making Machine / Injection Molding Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Loquat Juice and Loquat Syrup Production Machinery Export Worldwide]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Baby Polyester/Pu Coated Water-Resistant Outerwear]

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High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Folding Round Picnic Caravan Table RV Picnic Table Camping Table]

High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mechanical Seal 101 Type for Pump and Auto, Part I.Good Quality Pillow for Pregnant Wome Body Pregnant Pillow Case. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Walking Long Distance Surveying Instrument Meter Measuring Wheel]

Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA.2019 Newest Hybrid Shockproof Phone Case for iPhone Xs Max Xr X 8 7 6 Plus, 175-184[Shacman New 6X4 Weichai Engine Manual Dump Tipper Truck Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Micro DC 24V Worm Gear Brushless Motor for Medical Equipment]

Whitaker,J.R.(1963),Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine. Analytical Chemistry35(12), 1950-1953[Carboxymethyl Cellulose CAS No. 9004-32-4]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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