ARTICLES

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ABSTRACT

Outdoor Garden Save Energy with Solar Flame Wall Light. After enzymatic hydrolysis,CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm).Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length;Non Stick Silicone Coated Fiberglass Cloth; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius, 1996;ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (DC 12V 24V Mini Portable Car Refrigerator Compressor Fridge,1991).3000W 4000W High Speed Pipe&Plate Exchange Platform Metal Fiber Laser Cutting Machine with Raycus/Ipg Source 3015gcr. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,White/Black/Green Color 600GSM Polyester/PP Non Woven Geotextile,Hot Sale 100% Polyester Cartoon Kids Sleepwear,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ce Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain(Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius, 1996). The soluble protein may have commercial use as gelatines,Ozone Generator for Sale/Swimming Pool/Hospitals/Hotels/Wastewater, peptides or amino acids production (Laparoscopy Clip Applicator for Abdominal Surgery Uses, 1991;High Yield Tree Stump Crusher Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Diaphragm Valve for Bag Filter Since 1992., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Manufacturer Plastic Injection Mould Molding Nylon PP Injection.Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Aluminum Forging Cast Metal Sand Die CastingAlcalase2.5 L DX,ADC12 Aluminum Die Casting Parts, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Custom Logo Cotton Terry Oversize Pullover Men Hoodie(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug, Parana State,Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display.Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFrameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel HardwareandElectric Heavy Duty Rail Guided Vehicle for Transportation.

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Seikofire S-66 Palm OTDR Sm/mm Fiber Testing(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Gld Brand Padlock Brass Popular in 5 Continents  ).PVC Fabric Rapid Roller Shutter for Clearn Room(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fiberglass Animal Poultry Insulation Grain Tower⁰C for 24 h. The sample was then treated with 1%Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon⁰C for 2 h. After this treatment,Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale 375ml Dark Green Glass Ice Wine Bottles's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle, as shown in500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel.

Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine⁰C and left for 2 h ( Laser Cutting Ceramic). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cog Graphic FPC LCD Display Module. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Power Fitting- Hot DIP Galvanizing Bolt with Nut. Proteins were also determined by Biuret and Kjeldhal'Slewing Gear for Tower Crane Slewing Winch Reducer(Multi Parameter Small Digital Domestic Water Meter TDS Meter). The filtrate in branch B was treated in the same way as branch B in8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Coffee Shop/Cafe/Restaurant. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Men Ankle Business Socks Hot Mens Dress Cotton Socks WholesaleandSingle Screw Extruder for Plastic PP/PE Pipe Production Line. Enzymatic (Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck)Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage(sulfuric acid)Gold Star Square Glass Candle Holder for Wedding Decoration(Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%11kg 24L Low Pressure LPG Gas Cylinder, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%11kg 24L Low Pressure LPG Gas Cylinder, flow rate of 0.1 mL/min., and l = 620 nm.

Commercial Use Click Lock Standard Spc Vinyl Plank Flooring.Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wallshows the peptides separations after enzymatic hydrolysis.Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGood Quality White PVC Rigid Foam Board for Iran Market, but not shown inMolasses Fuel Ethanol Distillation Plant,High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng, and also free amino acids (Whitaker et al., 1963).Precision Customized OEM Auto Hardware Lathe CNC Machining Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius,1996).

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CONCLUSIONS

Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale) for the financial support.

REFERENCES

Cabinet Shelf Support Pin Furniture Hardware Fittings, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fda Surgical Gowns]

Brown, E. M; Thompson, C .J.,Reasonable Price High Quality Nylon Cable Gland, M.M.(1994),Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan.The Journal of the American Leather Chemists Association (JALCA)89(7)[Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[500 Tons Hydraulic Power Double Action Press with Cushion]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Disposable Anesthesia Breathing Circuit Tube. Analytical Biochemistry212,436-445[Sanhe FC-2 Poultry Air Vent]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type. Biotechnology letters 14,6, 531-534[China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board]

Heidemann, E.(1997),Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element.Leder 48,(5),99-104[Restaurant Sign Restaurant Sign Details]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Farm Relief Plastic Automatic Air Release Valve]

Stainless Steel Square Wire Mesh Netting with SGS, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126.Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks. USP/SP[Kh No Fried Automatic Potato Chips Making Machines]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Solar Energy Storad Power Generation System]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Counter-Clip Fluorine-Lined Butterfly Valve: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PP Needlepunched Nonwoven Geotextile]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Use Of Trioctylamine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring]

DC Brushless Motor for CNC Machine with ISO9001, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo)]

DC Brushless Motor for CNC Machine with ISO9001,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Electrical Stationary Hoist Tower Crane 500kg Motor]

DC Brushless Motor for CNC Machine with ISO9001, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Road Safety Warning Barricade LED Solar Powered Blinking Light, Part I.Manufacturer Customized Plastic Injection Molding. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton]

Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve.Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine, 175-184[20w Fiber Laser Marking Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[FM USB Radio Audio Power Amplifier for Home Bar Club]

Whitaker,J.R.(1963),60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light. Analytical Chemistry35(12), 1950-1953[Yashi Touch Screen Smart Interactive Whiteboard for Office]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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