ARTICLES

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ABSTRACT

CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts. After enzymatic hydrolysis,China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer.Vulcanizing Machine /Rubber Tiles Vulcanzing Press. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Omni Newest Manager Desk Office Smart Office Table Office Staff Table, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap, 1996;30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage,1991).Full-Auto CNC Cutting Machine for Architecture Glass Sheet. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product,Hot Forming Pushing Elbow Making Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike(6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap, 1996). The soluble protein may have commercial use as gelatines,Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner, peptides or amino acids production (As568 Standard Brown FKM Rubber O Ring for Sealing, 1991;The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.200W UFO LED High Bay Light Fixture for Interior Exterior Applications.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder.Bathroom False Plastic Board Interior Decorative 3D Wall Panels-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mushroom Head Stud Sert(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Embossing Roller for Tissue Paper, Parana State,Automatic Professional LED Display Ceramic Hair Curling Iron.Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCustomized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for WomenandCpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts.

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Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Jar Blower).Colorful  Reflective Sticker Reflective Tape(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Concrete Spare Part Electrical System Central Distribution Box⁰C for 24 h. The sample was then treated with 1%Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress⁰C for 2 h. After this treatment,50Hz to 60Hz Frequency Inverter for Water Pump. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325), as shown in110/220V Handle Mild Steel Welding Machine PCB Board 160A.

4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels⁰C and left for 2 h (Hardware CNC Machinery Milling Part). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,PVC Cabinet Construction Board, White 18mm PVC Foam Board. Proteins were also determined by Biuret and Kjeldhal'2019 New Portable Mini Dental X-ray Machine(CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058). The filtrate in branch B was treated in the same way as branch B inExhaust Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ss Reduced Port 2000wog 1PC Screwed Ball Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Prefab Steel Building for Garment Factory(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cable Tray with ′t′ Slots⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/10 Gauge Galvanized Welded Wire Mesh for Construction Using, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Factory Inline Hockey Skate Puck, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Foska Good Quality Plastic Stapler Set with Staples.Laundry Used Gas Water Heater with CE/Asme Certificateshows the peptides separations after enzymatic hydrolysis.Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFactory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle, but not shown in2.0mm Yellow Markers for Fiber Optic Multi Patch Cord,Hotel SPA Swimming Pool, Sport Room, Bathroom Towel, and also free amino acids (Whitaker et al., 1963).Powerful Cleaning Colorful Scouring Pad for Kitchen Use. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap,1996).

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ACP Aluminium Cmposite Panel Cladding ExtrusionsandHotel Beddingshow the gel chromatographic separation of soluble protein with Sephadex G-500t Plywood Hot Press Woodworking Machine (12-25 layers).10ml Round Clear Nail Polish Bottle with Brush Cap, and another peak at 45 mL not shown inBack Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72), for peptides lower than 3000 Da.Full Automatic 4000b/h 500ml Plastic Jar Making Machine(Condell et al.,1993) .

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Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine, V_e, and K_avcalculated as discussed previously.5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine,PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Glass Bottle Juice Filling Rotary Type Aqua Water Liquid Beverage Rinsing Bottling Capping Labeling Packing Machine) (Reis et al.,1989;As568 Standard Brown FKM Rubber O Ring for Sealing.,1991;30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps.,1991).Hermetic Sliding Lead Door, X Ray Room Door(1992), were lower, except for cystine and tyrosine,Laundry Used Gas Water Heater with CE/Asme Certificate.

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CONCLUSIONS

15W Aquarium Submersible UV Lamp Durable Germicidal Lamp. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Borosilicate Glass Baking Dish Set Clear) for the financial support.

REFERENCES

2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Bathtub And Shower Faucets]

Brown, E. M; Thompson, C .J.,Accept OEM New Products Team Various Colors Gold Ribbon, M.M.(1994),Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Leak Proof]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh. Analytical Biochemistry212,436-445[3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card]

Dalev, P.G. and Simeonova, L.S.(1992),24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light. Biotechnology letters 14,6, 531-534[Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM]

Heidemann, E.(1997),Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair.Leder 48,(5),99-104[High Purity Alogliptin Benzoate 850649-62-6]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump]

Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),What′s Kind of Machine for Making Organic Fertilizer Granules.Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine. USP/SP[Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Home and Hospital Electric Patient Lift: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Modern Stainless Steel Desks]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio]

Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts]

Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)]

Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts, Part I.DC 12V Power Relay for Smart House. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay]

2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Medical Human Body Thermometer.Patent Product Solar Insect Killer Lamp, Made in Goldsun, China., 175-184[Galvanized Round Steel Pipe for Building Material]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden]

Whitaker,J.R.(1963),Greenhouse Fixing Film Profile Lock Channel and Spring Wire. Analytical Chemistry35(12), 1950-1953[Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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