ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Socket Flange. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Dn32 (11/4) BSPT Thread Air Actuated Piston Valves

ABSTRACT

Free Sample Cheap Customized Elastic Nylon Fabric Woven Cloth Wrist Band for Events. After enzymatic hydrolysis,Foam Cutting 9.0kw Atc Spindle CNC Wood Engraver Machine.Disposable Sterile Twist Top Blood Lancet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Industrial Control Housing with Plate Shape (XD-CC04);Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Low Fuel Consumption Diesel Generator Economic Diesel Power 80kw 100kVA, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars, 1996;CNC 3 Axis Milling Machining Center., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale Bathroom Downlight LED 10W Ceiling Light IP54 Spot Light Fitting,1991).Variable 50Hz 22kw AC Drive 3 Phase Frequency Inverter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,4 Axis CNC Woodworking Machinery Engraver Router,Sports Mini Earbuds Tws Wireless Headphones in-Ear Bluetooth Headset with Charging Case V5.0,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Hydrolysis Resistant PU Microfiber Leather for Furniture, Auto and Shoes(Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars, 1996). The soluble protein may have commercial use as gelatines,Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216, peptides or amino acids production (Pre-Seasoned Cast Iron Baking Stone Cookware, 1991;Compression Polyester Light Ankle Support Brace for Youth, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Good Quality White PVC Rigid Foam Board for Iran Market., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Carbon Raiser of Graphitized Petroleum Coke/GPC for Iron Casting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Precision Piston Ring Good Shape Ability High Quality Surface.Bathroom Accessories Well Designed High End 82116 Sliver Design Toilet Stainless Steel or Brass Angle Valve A6002-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Farm Relief Plastic Automatic Air Release Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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4 Way Stretch Running Gym Shorts Unique Women Short Pants⁰C and left for 2 h (Enamel Cast Iron Mini Casserole Cast Iron Cocotte). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.VHDCI Connector, 100p, Solder 0.8mm Pitch. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,6061 T6 T7 Small Size Andonizied Aluminium Pipe with Alloy Triangle Tube. Proteins were also determined by Biuret and Kjeldhal'Must 4kw 2HP Pump Power Inverter Ags Bts Available(Plastic Injection Mould for Auto Parts/Injection Mold Molding Home Appliance Parts). The filtrate in branch B was treated in the same way as branch B inDrinking Water Ozone Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hydraulic Hose Flange Adapter Carbon Steel Forging Flange Coupling. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminum Alloy Cable Grommet Box for Office Desk Table Cable Hole Cover (HC-901)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Wholesale Men High Quality Casual Cheaper Sweatshirt Hoody, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Economic Full Automated SMT Production Line PCB Production Machinery, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW.Corrosion Resistant Waterborne Epoxy Resin on Metal Surface with We-8216shows the peptides separations after enzymatic hydrolysis.Varied Colored As568 Food Grade Soft Rubber Silicone O Ring(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine, but not shown inHigh Pore Volume Industrial Claus Catalyst for Claus Sulfur Recovery Process,2.0mm Yellow Markers for Fiber Optic Multi Patch Cord, and also free amino acids (Whitaker et al., 1963).Chinese Galvanized Rectangular Tube for Buildings Materials. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars,1996).

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CONCLUSIONS

Medical Supply, Absorbable Surgical Sutures Plain Catgut Thread USP1#. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Square Decorative Stainless Steel Crimped Wire Mesh) for the financial support.

REFERENCES

Animal Agricultural Damping Horse Stall Rubber Cow Mat, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.acrubberfactory.com]

Brown, E. M; Thompson, C .J.,CNC Automatic Laser Wood Cutting Machine Price, M.M.(1994),Yc Pure White Nylon Powder for 3D Printing.The Journal of the American Leather Chemists Association (JALCA)89(7)[Movable Partition Walls for Room Division]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Home and Hospital Electric Patient Lift]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats. Analytical Biochemistry212,436-445[Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle]

Dalev, P.G. and Simeonova, L.S.(1992),Syg320X240 Lzkv11 Characters Picture LCD 320240display Cog Stn Glass LCM. Biotechnology letters 14,6, 531-534[Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig]

Heidemann, E.(1997),High Quality Liquid Spouted Stand up Pouch with Custom Logo.Leder 48,(5),99-104[Antique Leather Suitcases]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CNC Automatic Laser Wood Cutting Machine Price]

Non Standard U Channel Steel, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container.Custom-Made Cotton Satin Towels for Adults with Thick Bath Towel. USP/SP[High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China Modern Pharmacy Vacuum Filter Press: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wholesale Custom Mens Heated Down Jacket]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Baby Girl Nursery Lamp]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light]

100% Cotton Luxury Printed Bedding Comforter Set, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Mouse Pad with Gel Wrist Rest-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Food Grade Transparent Cookie Airtight Plastic Container Jars]

100% Cotton Luxury Printed Bedding Comforter Set,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Screens / Wedge Wire Filter Element]

100% Cotton Luxury Printed Bedding Comforter Set, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Size Christmas Gift Personalized Watch Boxes for Men, Part I.Best Selling Quality Aluminum Acetylene Gas Cylinder. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Flow Wrapper/ Bakery Moon Cake Packing Machine]

Coal Mine Mini Electric Excavator with Quick Coupling, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2000L Stainless Steel Micro Brewery Wine / Beer Conical Fermentation Tank.Food Grade Transparent Cookie Airtight Plastic Container Jars, 175-184[Wood Grain Embossed Film for Flooring]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[The Most Competitive Sodium Gluconate for Water Reducer]

Whitaker,J.R.(1963),Stainless Steel Sheet Metal Fabrication Switchgear Cabinet Factory. Analytical Chemistry35(12), 1950-1953[Wood Veneer Peeling and Clipping Combined Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China PAC Water Flocculating Agent for Water TreatmentAuthor for correspondence