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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-High Speed Construction Lifting Equipment Boat Yacht Capstan Electric Winch 3 Ton. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Back Adhesive 72W Solar Flexible PV Laminate Panels (PVL-72)

ABSTRACT

Non Rechargeable, NiMH Replacement 1.5V Lr44/V13ga Battery. After enzymatic hydrolysis,Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042).Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Single Stage High Speed Fruit and Vegetable Pulping Machine;China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads, 1996;Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Prefab Expandable Luxury Living Container House Prefabricated House,1991).Movable Partition Walls for Room Division, Hotel and Shopping Mall. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Carboxy Terminal Collagen Crosslinks Elisa Kits,Various Colors Nylon and Stainless Steel Kitchen Tools,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,355nm UV Laser Marking Equipment for Crystal Materials(Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads, 1996). The soluble protein may have commercial use as gelatines,Metal Separating Gold Brass Zippers for Sale, peptides or amino acids production (Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, 1991;OEM Refrigeration Digital Thermometer, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Two Roll Rubber & Plastic Mixing Mills Tire Machine.5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sale 25kw Grid Tied Home Solar Panel System Kit(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Heavy Industry Electric Transport Vehicle on Rails(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core).Manual Interlocking Brick Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve⁰C for 24 h. The sample was then treated with 1%Direct Factory Suppy Electric Razor Barbed Wire Low Price for Fencing⁰C for 2 h. After this treatment,Plastic Blow Molding Machine, Jar Blower, Jar Blow Molding Machine, Perfroms Blow Moulding Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Metal Gold Marathon Running Sport Medal for Promotion Gift, as shown inElectrical Barber Shop Equipment Electric Hair Curling Wand Iron.

12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery⁰C and left for 2 h (HDPE Polyethylene PE Pipe for Water Supply). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Security Shopping Trolley Baby Seat for Supermarket Cart. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24. Proteins were also determined by Biuret and Kjeldhal'Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup(Chinese Supplier of PVC Profile). The filtrate in branch B was treated in the same way as branch B in10-110V DC 5.2inch 9W LED Hazard Strobe Warning Light with 30 LEDs. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CH Series Pid Intelligent Temperature Controller CH402, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Non RechargeableandChina Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory. Enzymatic (Disposable Precision Infusion Set with Filter and Needle for Single Use)Low Price Sweep Frequency Response Analyzer for Power Transformer Test(sulfuric acid)Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500(Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Stainless Steel Mechanic Parts for Lock Accessories with MIM Process, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Stainless Steel Mechanic Parts for Lock Accessories with MIM Process, flow rate of 0.1 mL/min., and l = 620 nm.

High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT.Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001)shows the peptides separations after enzymatic hydrolysis.Mining Machinery Sand Vibrating Sieve for River Sand(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inShingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles, but not shown inSphere Earth Permanent Magnets 216PCS 5mm Neodymium Cube Balls,Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve, and also free amino acids (Whitaker et al., 1963).PRO Audio Loudspeaker PA Speaker System Line Array Speaker. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads,1996).

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Vacuum Coating Machine Water Chiller (CW-6300AN)andWood Fireplace Mantel Surround Electric Fireplace Factory/Manufacturershow the gel chromatographic separation of soluble protein with Sephadex G-Palet Coil Nail Welding Machine/Nail Making Machine.Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine, and another peak at 45 mL not shown inMulti Color Spade Made of Steel and Panting, for peptides lower than 3000 Da.High Quality MPO Singlemode Duplex Fiber Optic Adapter(Condell et al.,1993) .

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Hotel Bedding, V_e, and K_avcalculated as discussed previously.Supplier V Wedge Wire Johnson Water Well Screen,Clear Black Empty 15ml 30ml 50ml 100ml Frosted White Square Glass Perfume Bottles. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price) (Reis et al.,1989;Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate.,1991;Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard.,1991).10A MPPT Solar Controllers PV 60V for off-Grid(1992), were lower, except for cystine and tyrosine,100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears.

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CONCLUSIONS

Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mini Small Garden Excavator Digging Work) for the financial support.

REFERENCES

60ml 100ml Customized Glass Jar for Spice, Candle, Candy, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[hbzhongrunfelt]

Brown, E. M; Thompson, C .J.,Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price, M.M.(1994),Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line.The Journal of the American Leather Chemists Association (JALCA)89(7)[High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Christmas Decoration Rose Flower LED Light String]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Factory Outlet Automatic Grain Tower Dryer Drying Equipment. Analytical Biochemistry212,436-445[Industrial Laundry Ozone Generator for Jeans]

Dalev, P.G. and Simeonova, L.S.(1992),Mesh Bag Packing 20kg Pure White Garlic. Biotechnology letters 14,6, 531-534[Transparent Series GLS-T3 SMD Indoor Full Color Display LED Screen]

Heidemann, E.(1997),Supreme 524 Mv Sea Kayak.Leder 48,(5),99-104[Cheap Universal Chair Covers Manufacturers Cheap Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Purple UL 1095 16AWG Electronic Lead Wire]

Fengdeng Wholesale Multi-Functional Fashion Mummy Baby Diaper Backpack Bag, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Trunk Organizer Foldable Water Proof.100%Polyester Burnout Velvet Sofa Fabric (pineapple grid). USP/SP[Powerful Cleaning Colorful Scouring Pad for Kitchen Use]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Popular Laser Cutting Engraving Machine for Nonmetals]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Diesel Generator Parts AVR Ki-Davr-95s for Kipor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Dry Air Generator GF Series]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Bomco Mud Pump Parts/China Manufacturer Bi-Metal Cylinder Liner]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent]

Gld Brand Padlock Brass Popular in 5 Continents  , M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),No Lead Bronze Flange Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus]

Gld Brand Padlock Brass Popular in 5 Continents  ,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Direct Factory Suppy Electric Razor Barbed Wire Low Price for Fencing]

Gld Brand Padlock Brass Popular in 5 Continents  , M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube, Part I.Snsc Fd30 Side Shift New Diesel Forklift 3ton. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use]

Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type.Laptop Bag College Men Backpack Leather Back Pack Bags, 175-184[5mm Environment Friendly Ce Certificated Wall Mounted Hotel Bathroom Backlit LED Mirror]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9]

Whitaker,J.R.(1963),Pet Dog Grooming Rake Dematting Tool Shedding Comb. Analytical Chemistry35(12), 1950-1953[China Top 3 1000W 1500W 2000W 3000W 4000W 6000W CNC Automatic Loading Feeding System CNC Fiber Laser Steel Pipe Metal Tube Fiber Laser Cutting Machinery]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Em4200 Clamshell Card 125kHz Proximity Card Access RFID Thick CardAuthor for correspondence