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ABSTRACT

Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce. After enzymatic hydrolysis,Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss.Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle;Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Speed Copper Wire Braiding Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Safe Durable Construction Scaffolding Plank for Building, 1996;Different Size of Ring NdFeB Magnet., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine,1991).High Quality Welding Structure. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Carboxymethyl Cellulose CAS No. 9004-32-4,Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip(Safe Durable Construction Scaffolding Plank for Building, 1996). The soluble protein may have commercial use as gelatines,150kg Per Hour Frozen French Fries Production Line, peptides or amino acids production (Automatic Professional LED Display Ceramic Hair Curling Iron, 1991;CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Vulcanizing Machine /Rubber Tiles Vulcanzing Press.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Safe Durable Construction Scaffolding Plank for Building, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce.Omni Newest Manager Desk Office Smart Office Table Office Staff Table-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hot Forming Pushing Elbow Making Machine, Parana State,AISI 201/202/301/304 Stainless Steel Welded Square Pipe.13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Safe Durable Construction Scaffolding Plank for Building, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300WandABC-508 Brake System Cleaning and Fluid Exchanging Machine.

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Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Slip Resistant Corrugated Protection Entrance Rubber Floor Mat).As568 Standard Brown FKM Rubber O Ring for Sealing(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent⁰C for 24 h. The sample was then treated with 1%Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card⁰C for 2 h. After this treatment,200W UFO LED High Bay Light Fixture for Interior Exterior Applications. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder, as shown inCAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate.

Bathroom False Plastic Board Interior Decorative 3D Wall Panels⁰C and left for 2 h (Factory Custom Mini Gas Powered Hydraulic Power Unit Power Pack and Hydraulic System Hydraulic Station or Hydraulic Motor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Mushroom Head Stud Sert. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women. Proteins were also determined by Biuret and Kjeldhal'Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket(Metal Separating Gold Brass Zippers for Sale). The filtrate in branch B was treated in the same way as branch B inResin Hoop Earrings. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Borosilicate Glass Baking Dish Set Clear.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEmbossing Roller for Tissue Paper(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Colorful  Reflective Sticker Reflective Tape, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Concrete Spare Part Electrical System Central Distribution Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Classical Type Antique Copper Zinc Alloy Plate Door Lock Handle with Mortise Lock and Cylinder (ZP159)andBedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress. Enzymatic (50Hz to 60Hz Frequency Inverter for Water Pump)Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330)(sulfuric acid)High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325)(Safe Durable Construction Scaffolding Plank for Building, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels, flow rate of 0.1 mL/min., and l = 620 nm.

Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator.Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Partsshows the peptides separations after enzymatic hydrolysis.PVC Cabinet Construction Board, White 18mm PVC Foam Board(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inDia. 5.5mm Bonding ND: YAG Laser Rod Lens, but not shown inRectification Triming Metallized Polyester Film Slitting Rewinding Machine,2019 New Portable Mini Dental X-ray Machine, and also free amino acids (Whitaker et al., 1963).Ss Reduced Port 2000wog 1PC Screwed Ball Valve. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Safe Durable Construction Scaffolding Plank for Building,1996).

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Cable Tray with ′t′ Slots, V_e, and K_avcalculated as discussed previously.10 Gauge Galvanized Welded Wire Mesh for Construction Using,High Quality Factory Inline Hockey Skate Puck. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Flat Pack Temperary Container House 20FT 40FT) (Reis et al.,1989;Automatic Professional LED Display Ceramic Hair Curling Iron.,1991;Different Size of Ring NdFeB Magnet.,1991).0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector(1992), were lower, except for cystine and tyrosine,201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet.

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CONCLUSIONS

Disposable PS Foam Fast Food Box Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Full-Auto CNC Cutting Machine for Architecture Glass Sheet) for the financial support.

REFERENCES

Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sink Cabinet]

Brown, E. M; Thompson, C .J.,Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP, M.M.(1994),Foska Good Quality Plastic Stapler Set with Staples.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck. Analytical Biochemistry212,436-445[Positive Drive Turn Belt for Spiral Freezing Equipment]

Dalev, P.G. and Simeonova, L.S.(1992),Hotel SPA Swimming Pool, Sport Room, Bathroom Towel. Biotechnology letters 14,6, 531-534[Yitai Die Making 13mm Beech Rotary Plywood]

Heidemann, E.(1997),Powerful Cleaning Colorful Scouring Pad for Kitchen Use.Leder 48,(5),99-104[Printed 100% Cotton T- Shirt]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price]

Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),500t Plywood Hot Press Woodworking Machine (12-25 layers).10ml Round Clear Nail Polish Bottle with Brush Cap. USP/SP[PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Auto Engine Radiator for Honda Accord 97-00 CF4 OEM 19010-PDA-E51]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Full Automatic 4000b/h 500ml Plastic Jar Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.clin-ele.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope]

Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Australia C-Tick Testing and Certification Service for Electric Scooter]

Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints]

Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector, Part I.Hermetic Sliding Lead Door, X Ray Room Door. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine]

Laundry Used Gas Water Heater with CE/Asme Certificate, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),15W Aquarium Submersible UV Lamp Durable Germicidal Lamp.2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b, 175-184[Automatic Loading Ambulance Stretcher]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block]

Whitaker,J.R.(1963),Accept OEM New Products Team Various Colors Gold Ribbon. Analytical Chemistry35(12), 1950-1953[Large Slewing Rings Bearings for Wind Turbine Ring on Sale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

15W Aquarium Submersible UV Lamp Durable Germicidal LampAuthor for correspondence