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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Cable Tray with ′t′ Slots. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,10 Gauge Galvanized Welded Wire Mesh for Construction Using

ABSTRACT

High Quality Factory Inline Hockey Skate Puck. After enzymatic hydrolysis,0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector.201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Disposable PS Foam Fast Food Box Making Machine;Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Foska Good Quality Plastic Stapler Set with Staples, 1996;Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hotel SPA Swimming Pool, Sport Room, Bathroom Towel,1991).Powerful Cleaning Colorful Scouring Pad for Kitchen Use. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,500t Plywood Hot Press Woodworking Machine (12-25 layers),10ml Round Clear Nail Polish Bottle with Brush Cap,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Full Automatic 4000b/h 500ml Plastic Jar Making Machine(Foska Good Quality Plastic Stapler Set with Staples, 1996). The soluble protein may have commercial use as gelatines,Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine, peptides or amino acids production (5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine, 1991;PCB18h 8p8c Modular Plug with Shield Head RJ45 Connector, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hermetic Sliding Lead Door, X Ray Room Door., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Laundry Used Gas Water Heater with CE/Asme Certificate.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-15W Aquarium Submersible UV Lamp Durable Germicidal Lamp. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Foska Good Quality Plastic Stapler Set with Staples, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b.Accept OEM New Products Team Various Colors Gold Ribbon-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal MeshAlcalase2.5 L DX,24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)What′s Kind of Machine for Making Organic Fertilizer Granules(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine, Parana State,10 Gauge Galvanized Welded Wire Mesh for Construction Using.Home and Hospital Electric Patient Lift, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Foska Good Quality Plastic Stapler Set with Staples, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCrushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002)andHigh Pressure Fitting Quick Coupling Hydraulic Plug.

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Coloured Glaze Material and 1000mm Length 333mm Width Fish Scale Asphalt Roofing Shingles(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Hv / Coating Material Grade CMC Medium Viscosity).M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts⁰C for 24 h. The sample was then treated with 1%DC 12V Power Relay for Smart House⁰C for 2 h. After this treatment,2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Medical Human Body Thermometer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Patent Product Solar Insect Killer Lamp, Made in Goldsun, China., as shown inHigh Efficiency Fine Powder Vibrating Screener.

Greenhouse Fixing Film Profile Lock Channel and Spring Wire⁰C and left for 2 h (Size0/Color/Organic/Pullulan/HPMC/Gel/Hollow/Veggie/Transparent/Clear/Empty Capsules). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Export Quality Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle. Proteins were also determined by Biuret and Kjeldhal'RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc(3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry). The filtrate in branch B was treated in the same way as branch B inwww.waterdroprofilter.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,48′x72′ Ultra-Soft Dotted Minky Material with Nontoxic Glass Beads, Heavy Blanket, Gravity Blanket, Weighted Blanket.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hydrophobic Excellent Thermal Insulation Silica Aerogel Powder. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStrong Motor Permanent Neodymium Rare Earth Bar Magnet N52(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Glass Cutting Table/CNC Glass Cutting Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/12 Bottles Portable Home Ues Thermoelectric Wine Chiller, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filter, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water TankandPortable Mini USB Charging Flashlight 10W T6 LED Flashlight (SYSG-190321). Enzymatic (Brushed Cotton Twill Fine 3D Embroidery Custom Baseball Cap)Robotic Fastening Tool/Desktop 4-Axis Screw Fastening Robot  /Instead of Manual Fastening(sulfuric acid)Wholesale Logo Printed Recycled Retail Paper Shopping Bag with Handle(Foska Good Quality Plastic Stapler Set with Staples, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Black Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe, flow rate of 0.1 mL/min., and l = 620 nm.

Stainless Steel Coil Heat Exchanger Industrial Water Cooler Stainless Steel Heat Exchanger.Double Roller Rotary Wood Peeling Machine with CE for Saleshows the peptides separations after enzymatic hydrolysis.Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter, but not shown inFactory Price High Carbon Isostatic Graphite Electrodes,Q35y-30 Ironworker Shearing and Punching Machine Ce Approved Q35y Hydraulic Iron, and also free amino acids (Whitaker et al., 1963).Shenzhen Yizexin Numeric Keyboard Conductive Ink Membrane Switch. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Foska Good Quality Plastic Stapler Set with Staples,1996).

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E171 Standrad Titanium Dioxide Food Grade A200andHigh Efficiency Non-Expensive Cracking Agentshow the gel chromatographic separation of soluble protein with Sephadex G-High-Tenacity Conveyor Belt/ Steel Cord Conveyor Belt.Hanwha SMT Chip Shooter LED Pick and Place Machine, and another peak at 45 mL not shown inVenlo Type Flower Multi Span Commercial Glass Greenhouses Used, for peptides lower than 3000 Da.ACP Aluminium Cmposite Panel Cladding Extrusions(Condell et al.,1993) .

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New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine, V_e, and K_avcalculated as discussed previously.Wholesale Custom Mens Heated Down Jacket,Rectification Triming Metallized Polyester Film Slitting Rewinding Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer) (Reis et al.,1989;5L, 8L, 10L, 20L, 50L, 100L Laboratory Cone Shape Dry Powder Mixer /Blending /Mixing /Blend /Blender Machine.,1991;Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck.,1991).Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker(1992), were lower, except for cystine and tyrosine,New Swim Shorts Grey Mens Board Shorts with Mesh Liner.

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CONCLUSIONS

Multifunction CNC Woodworking Machine with 1300*2450*200mm Working Area, 9kw Air Cooling Spindle, Nc Studio Control. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair) for the financial support.

REFERENCES

Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Gentleman Belt Vintage Cowhide Leather Belt Classical Formal Dress Men Belt]

Brown, E. M; Thompson, C .J.,Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil, M.M.(1994),Marine Mooring Hardware, Anchor Chain.The Journal of the American Leather Chemists Association (JALCA)89(7)[60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Cog Graphic FPC LCD Display Module]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls. Analytical Biochemistry212,436-445[2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b]

Dalev, P.G. and Simeonova, L.S.(1992),Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick. Biotechnology letters 14,6, 531-534[K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask]

Heidemann, E.(1997),QJ-M Moving small manual brick making machine with low cost.Leder 48,(5),99-104[hbzhongrunfelt]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[24 Volt Fire Alarm Strobe Light Horn Strobe Sounder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Braiding Machine for Weaving Flexible Metal Hose: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[YAMAHA Yv100II Nozzle Horder Square Head Short Type]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.liushtech.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel]

Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fashion Metal Button Crystal Shank Button for Garment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Corrugated Board Production Line]

Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[T90 High Power Latching Relay Mini Power Relay]

Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616), Part I.10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe]

Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),ABC-508 Brake System Cleaning and Fluid Exchanging Machine.Polypropylene Woven Bag for Sludge Dewatering, 175-184[Cationic PAM Coagulant for Water Treatment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Safe Durable Construction Scaffolding Plank for Building]

Whitaker,J.R.(1963),China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale. Analytical Chemistry35(12), 1950-1953[Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117)Author for correspondence