ARTICLES

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ABSTRACT

Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure. After enzymatic hydrolysis,80W All in One Outdoor LED Street Light Solar Lamp.Hot Sale Safety Vinyl PVC Flooring. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation;Horizental Stainless Steel Food Grade Milk Tank for Milking; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Multi Parameter Small Digital Domestic Water Meter TDS Meter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display, 1996;Sanhe FC-2 Poultry Air Vent., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Picosecond Laser Tattoo Removal Skin Care Medical Instrument,1991).XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849,Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,SUS316 Hexagon Socket Set Screws with Cone Point, DIN914(27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display, 1996). The soluble protein may have commercial use as gelatines,Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale, peptides or amino acids production (Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor, 1991;Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Explosion-Proof Housing Sealing Dispensing Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Tube Mills for Thin Wall Tubing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Car Badge Custom Emblem Auto Logo Sign for Advertising.2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Silver 8 Inch Double Thick Round Cake Board(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Agricultual Hand Sprayer/Garden Hand Sprayer /Home Hand Sprayer (TF-01F)).High Precision Machining Auto Parts Metal Contact(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label⁰C for 24 h. The sample was then treated with 1%Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler⁰C for 2 h. After this treatment,Paper Sticky Lint Remover Roller/Pet Hair Roller. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Safe Durable Scaffolding Trapdoor Plank for Construction's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display, as shown inHigh Quality Aluminum Heat Sink for Electronics Computer Electric.

Various Sublimation Blank Home Supply Fabric Bath Towel etc⁰C and left for 2 h (Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments. Proteins were also determined by Biuret and Kjeldhal'Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube(Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag). The filtrate in branch B was treated in the same way as branch B inMedia and Activated Carbon Filters for Pure Water Needs. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Best Quality Bending Metal Mould with All Kinds Metal Materials.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMilling Precision Stainless Steel CNC Machinery Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Good Price Stand-up Zipper Doy Pouch Filling Packing Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Mint Flavor 50ml Home Foam Toothpaste.Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042)shows the peptides separations after enzymatic hydrolysis.125mmsintered Dry Cutter Turbo Diamond Circular Saw Blade for Tile(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station, but not shown inCustomized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use,Dxz Limit Switch Tower Crane Spare Parts, and also free amino acids (Whitaker et al., 1963).OEM/ODM Price Top Open Keypad Safe with Electronic Code Lock. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display,1996).

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CONCLUSIONS

Electric Magic Hot Hair Styling Curling Iron with Brushes. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow) for the financial support.

REFERENCES

Melt Pressure Transducer (PT133), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Gear Rack and Pinion for Construction Hoist]

Brown, E. M; Thompson, C .J.,Galvanized Zinc/Aluminum Structure Steel Square Tube/ Round Pipe, M.M.(1994),Women Genuine Crazy Horse Cowhide Leather Tassel Crossbody Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[Motorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Tower 6-Axis Screwdriver Machine Robot Manufacturer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine. Analytical Biochemistry212,436-445[Food Grade Material 304/316 Double Diaphragm Pumps]

Dalev, P.G. and Simeonova, L.S.(1992),Educational Toys, Kid Eduactional Toys House, Children Educational Toys Game. Biotechnology letters 14,6, 531-534[Patent Product Solar Insect Killer Lamp]

Heidemann, E.(1997),Passive RFID Sticker Label UHF-Tag Alien 915MHz 868MHz 6c EPC Wet-Inlay H3-9662.Leder 48,(5),99-104[White Flower Puff Print Cropped Tee with Short Sleeves for Women]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PE Film Self Adhesive Bitumen Waterproof Membrane/ Roofing Material]

Plastic Injection Moulding Machine Supplier / Factory /Manufacturer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Auto Spare Parts for Water Pump 19200-Rdv-J01 for Accord.Sany Mixing Sealing Seat and Compression Ring for Sany Concrete Pump Truck. USP/SP[Manual Foot Pedal Hydraulic Pump]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Automatic Professional LED Display Ceramic Hair Curling Iron]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Durable Aluminum Flag Pole with Dome Top Tangle Free Finial: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality Office Air Purifier Plastic Injection Mould Maker]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies]

Customized Marathon Medal for Marathon Medallion, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Price of a Retail Supermarket Push Cart for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Personal Care Glutathione Skin Whitening Tablets]

Customized Marathon Medal for Marathon Medallion,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant]

Customized Marathon Medal for Marathon Medallion, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic Pan Feeding Line and Poultry Nipple Drinking System for Chicken Broiler and Breeder, Part I.Lady Kids Child Yoga Clothes Gym Fitness Sports Wear. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Manufacture Best Choice Liquid Flocculant for Ink Removal]

Hotel Steel Digital Room Safes with Large LED Display, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Vintage Antique Home Living Decor Ceiling Crystal Chandelier Pendant Lighting Fixture.Biogas/Methane Purification System/Desulfurization/Scrubber Tower, 175-184[www.meditecho2.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type]

Whitaker,J.R.(1963),Fast Supplier Juice Sealing Film Wrapping Packing Machine Production Line. Analytical Chemistry35(12), 1950-1953[Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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