ARTICLES

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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Personal Care Glutathione Skin Whitening Tablets, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customized Over Size Boys Hoodie with Embroidered, 1996;Digital Printing Football Fans Flag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors,1991).Flb-80125AC Automatic L Type Sealer Sealing Heat Hot Shrink Shrinking Shrinkable Film Wrap Wrapper Wrapping Pack Packing Packer Package Packaging Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,PTFE+Bronze Shaft Rod Step Seal Hbts/Spgo Buffer Seal,500 Tons Hydraulic Power Double Action Press with Cushion,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine(Customized Over Size Boys Hoodie with Embroidered, 1996). The soluble protein may have commercial use as gelatines,Promotional Plastic Laminated Custom Shopping PP Woven Bag, peptides or amino acids production (Metal Galvanized Wave Form Perforated Steel Strips, 1991;Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Silver Anodized Aluminium I Beam Level Measuring Tool. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customized Over Size Boys Hoodie with Embroidered, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring.48V25A Lead Acid Battery Maintenance Pulse Charger-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:15mm Best Price Commercial Grade Birch Plywood for Furniture(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Anchor Chain).Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine⁰C for 24 h. The sample was then treated with 1%Stainless Steel Clarifier Tank for Water Treatment⁰C for 2 h. After this treatment,Carrier Roller for Sany Excavator. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine, as shown inHotel SPA Swimming Pool.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDry Air Generator GF Series(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Advanced Unlocking Tool for Locksmith⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Screen Printing Materials and Equipments Screen Printing Frames, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Supplier Hard Ware Fittings Gas Spring for Tool Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road, V_e, and K_avcalculated as discussed previously.High Speed Copper Wire Braiding Making Machine,Safe Durable Construction Scaffolding Plank for Building. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts) (Reis et al.,1989;Metal Galvanized Wave Form Perforated Steel Strips.,1991;Digital Printing Football Fans Flag.,1991).Different Size of Ring NdFeB Magnet(1992), were lower, except for cystine and tyrosine,Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine.

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CONCLUSIONS

High Quality Welding Structure. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing) for the financial support.

REFERENCES

Carboxymethyl Cellulose CAS No. 9004-32-4, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cfrp Carbon Fiber Triangle Tube]

Brown, E. M; Thompson, C .J.,Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher, M.M.(1994),Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip.The Journal of the American Leather Chemists Association (JALCA)89(7)[Parcel Tape Adhesive OPP Packing Tape]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Single Screw Extruder for Plastic PP/PE Pipe Production Line]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-150kg Per Hour Frozen French Fries Production Line. Analytical Biochemistry212,436-445[Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief]

Dalev, P.G. and Simeonova, L.S.(1992),Automatic Professional LED Display Ceramic Hair Curling Iron. Biotechnology letters 14,6, 531-534[30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products]

Heidemann, E.(1997),CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts.Leder 48,(5),99-104[Bathroom Wall Covering Panels]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Supplier Hard Ware Fittings Gas Spring for Tool Box]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Brushed Anodizing Aluminum Extrusion Profile for LED]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[4 Layers PCBA Circuit Board Fabrication PCB Assembly Manufacture]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM]

Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Omni Newest Manager Desk Office Smart Office Table Office Staff Table-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift]

Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror]

Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap, Part I.30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Spare Parts for Caterpillar Construction Machinery]

Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Full-Auto CNC Cutting Machine for Architecture Glass Sheet.Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product, 175-184[Polyester Anti-Static Needle Felt (Blend Yarn)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine]

Whitaker,J.R.(1963),Hot Forming Pushing Elbow Making Machine. Analytical Chemistry35(12), 1950-1953[90d Saf2205 Duplex 2205 Seamless Stainless Steel Dn50 Elbow]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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