ARTICLES

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ABSTRACT

Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck. After enzymatic hydrolysis,Union Metal Steel Building and Construction Material.Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;450ml Sublimation Stainless Steel Milk Bottle (Colorful);Lower Noise Colled Rolled Ball Screw; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sphere Earth Permanent Magnets 216PCS 5mm Neodymium Cube Balls, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3.0kw Spindle CNC Router with Certificate for Woodworking, 1996;800W Electric Motor Price Lawn Mower Motor Concrete Mixer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Air Cooled Split Type Ducted Air Conditioner AC Unit,1991).Hans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvet,Gym Fitness Equipment Adjustable Weight Bench,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Factory Sell P7.8 Transparent LED Display Screen(3.0kw Spindle CNC Router with Certificate for Woodworking, 1996). The soluble protein may have commercial use as gelatines,Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V), peptides or amino acids production (Original Weichai Engine Truck Spare Parts Valve Cap 61500050105, 1991;Sliding Panel Saw Heavy Duty CNC Band Saw for Furniture Ban Resaw Machine for Lumber, Round Log Slinding Sawmill, Band Resaw for Lumber, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ceramic Edge Protection Profiles Decorative Ceramic Metal Tile Trim., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Charms for Jewelry Making (Sold Per Bag 10PCS Inside).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fused Quartz Powder 325mesh 500mesh 1250mesh. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3.0kw Spindle CNC Router with Certificate for Woodworking, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Various Types of Plastic Hooks Mould From Manufacturers.New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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E171 Standrad Titanium Dioxide Food Grade A200(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Best Quality Bending Metal Mould with All Kinds Metal Materials).Elbow Forming Machine Gorelocker HVAC Duct Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Barbecue Net BBQ Grill Grates Wire Mesh⁰C for 24 h. The sample was then treated with 1%Best Selling Colorful Branded Custom Logo Polarized Sunglasses Women Men 2018 Fashion UV400 Cat3 Mirrored Sun Shade Glasses⁰C for 2 h. After this treatment,L Shape U Shape Big Corner Home Villa Living Room Hotel Lobby Full Chinese Italy Leather with Manual Movable Headrest Confortable Sofa Set Furniture. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)304 Stainless Steel Anti-Corrosion Welded Wire Mesh, as shown inSPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF.

Custom Made Colorful Sublimation Womens Yoga Pants Wholesale⁰C and left for 2 h (48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Outdoor Garden Lamp Remote Control with Polycrystalline Silicon Solar Panel. Proteins were also determined by Biuret and Kjeldhal'New Fashion PVC Sequins Adjustable Women Brace Suspenders(Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor). The filtrate in branch B was treated in the same way as branch B inISO9001 5kg 12L Low Pressure LPG Gas Cylinder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Solid Flex-Drain Pipe Length 40′.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avin Ear Tws I9 Mini Bluetooths Earbuds with Charging Case(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cake/Cookies/Bread Automatic Package and Packing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customize Milky Extrusion PC Cover, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Customized Plastic Items Access Flap Cover Cap Plastic Injection MouldingandEuropean Good Quality Bathroom Simple Sliding Glass Shower Cabina. Enzymatic (UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone)Fruit Plum Cleaning Washing Machine with Brushes Spray(sulfuric acid)Professional Manufacture Electromagnetic Impulse Solenoid Valve, Pulse Operated Solenoid Valve for Dust Collector(3.0kw Spindle CNC Router with Certificate for Woodworking, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%11.1V 2000mAh Lithium Polymer Battery Pack Customized Size, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%11.1V 2000mAh Lithium Polymer Battery Pack Customized Size, flow rate of 0.1 mL/min., and l = 620 nm.

Buy Mk 677 Powder Bulk Powders Sarms Mk 677, Mk677.Fashion Shoulder Backpack Handbag School Bagshows the peptides separations after enzymatic hydrolysis.Jh21 Series Pneumatic Power Press CNC Punching Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHeavy Duty Rope12 Strand PP PE Mixed Pull Mooring Mope, but not shown inSecurity Shopping Trolley Baby Seat for Supermarket Cart,Coal Fried Steam Boiler for Industry with Double Drum, and also free amino acids (Whitaker et al., 1963).Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3.0kw Spindle CNC Router with Certificate for Woodworking,1996).

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Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production, V_e, and K_avcalculated as discussed previously.Slip Resistant Corrugated Protection Entrance Rubber Floor Mat,Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Sw Type BOPP Packaging Adhesive Tape Coating Machine) (Reis et al.,1989;Original Weichai Engine Truck Spare Parts Valve Cap 61500050105.,1991;800W Electric Motor Price Lawn Mower Motor Concrete Mixer.,1991).T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm)(1992), were lower, except for cystine and tyrosine,High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine.

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CONCLUSIONS

Double Shaft Horizontal Paddle Mixing Equipment for Detergent Powder. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Soft Baby Suites Dinosaure Pijamas Kigurumi) for the financial support.

REFERENCES

Mechanical Seal 101 Type for Pump and Auto, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Lithium Battery Bl1860 Used Makita Cordless Drill Electric Power Tools]

Brown, E. M; Thompson, C .J.,Good Quality Pillow for Pregnant Wome Body Pregnant Pillow Case, M.M.(1994),Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass.The Journal of the American Leather Chemists Association (JALCA)89(7)[12 Bottles Portable Home Ues Thermoelectric Wine Chiller]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fused Quartz Powder 325mesh 500mesh 1250mesh]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA. Analytical Biochemistry212,436-445[China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2)]

Dalev, P.G. and Simeonova, L.S.(1992),2019 Newest Hybrid Shockproof Phone Case for iPhone Xs Max Xr X 8 7 6 Plus. Biotechnology letters 14,6, 531-534[Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16]

Heidemann, E.(1997),Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine.Leder 48,(5),99-104[Cheap Bmx Pedal]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ X Ray Room Door]

Hot Sell Mechanical Bar Screen for Water Treatment, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Ipg 1000W, 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube.3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board. USP/SP[Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Low Pressure PU Filter Gasket Sandal Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Plastic Mold Maker]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine]

Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Flour Making Machine Wheat Maize Flour Mill Machinery-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Flour Making Machine Wheat Maize Flour Mill Machinery]

Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns]

Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens, Part I.Factory Direct Supply Yiwu Stocklots Hard Cover A5 High Quality Leather Notebooks for Gifts Free Shipping. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[80W All in One Outdoor LED Street Light Solar Lamp]

Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment.Metal Material and Sports Theme Custom Metal Medal, 175-184[Flower Wall Paper]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery]

Whitaker,J.R.(1963),Portable Installation and Ce Certification 20g/Hr OEM Air Purifier. Analytical Chemistry35(12), 1950-1953[Explosion-Proof Housing Sealing Dispensing Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Easy Operational Cup Cake Filling MachineAuthor for correspondence