ARTICLES

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ABSTRACT

Precision Customized OEM Auto Hardware Lathe CNC Machining Parts. After enzymatic hydrolysis,White Color Enamel Bowls with High Quality.Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line;Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Southeast Asia Healthy Soft Integrated Latex Foam Mattress, 1996;12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Water Filter System Lamella Clarifier for River Water Treatment System,1991).Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cabinet Shelf Support Pin Furniture Hardware Fittings,Reasonable Price High Quality Nylon Cable Gland,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan(Southeast Asia Healthy Soft Integrated Latex Foam Mattress, 1996). The soluble protein may have commercial use as gelatines,Disposable Anesthesia Breathing Circuit Tube, peptides or amino acids production (High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type, 1991;Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Counter-Clip Fluorine-Lined Butterfly Valve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Southeast Asia Healthy Soft Integrated Latex Foam Mattress, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰DC Brushless Motor for CNC Machine with ISO9001.Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Road Safety Warning Barricade LED Solar Powered Blinking Light(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (E171 Standrad Titanium Dioxide Food Grade A200).Custom Aluminum Cover Frame Half Aluminum Cover Profile(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility⁰C for 24 h. The sample was then treated with 1%DC24V 36W Solar Fan Motor PCBA Card⁰C for 2 h. After this treatment,New Monocular Hunting Wargame Mini Scope Digital Night Vision. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor, as shown inHigh Efficiency Non-Expensive Cracking Agent.

Italy Design Good Quality Hydraulic Motorcycle Lift Table⁰C and left for 2 h (Venlo Type Flower Multi Span Commercial Glass Greenhouses Used). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Menthol E Liquid E Juice for EGO E Cigarettes. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids. Proteins were also determined by Biuret and Kjeldhal'New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9(What′s Kind of Machine for Making Organic Fertilizer Granules). The filtrate in branch B was treated in the same way as branch B inA5 Child Book Printing. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Portable Telescopic Straw Metal Drinking Straw with Brush Case.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEtching Rear Trunk Sill Plates for Hyundai Tucson(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Lab Equipment Sr Paper Pulp Beating Freeness Test Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Xsg Series High-Speed Rotating Dryer Putty Powder Special DryerandPopular Water Bike / Hydro Bike / Aqua Bike (SK-8001). Enzymatic (Fertilizer Granulator Machine, Compound Fertilizer & Organic Fertilizer Granulator, Machine for Making)Double Roller Rotary Wood Peeling Machine with CE for Sale(sulfuric acid)5in1 USB Type C Hub HDMI 4K USB C Hub to Ethernet RJ45 LAN Adapter(Southeast Asia Healthy Soft Integrated Latex Foam Mattress, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070), flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070), flow rate of 0.1 mL/min., and l = 620 nm.

Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone.Evaporator Finned Tubeshows the peptides separations after enzymatic hydrolysis.Ladle Refining Melting Steel Furnace Casting Ladle(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMagnesium Sulphate/ Chemical Fertilizer Magnesium / Aquaculture Addtive, but not shown in60W All-in-One PIR Sensor Die-Aluminum Solar Street Light Price,Lishui Factory High Quality Metal Rolling Ball Screw, and also free amino acids (Whitaker et al., 1963).Y-Spring Threaded Check Valve, 800 Wog. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Southeast Asia Healthy Soft Integrated Latex Foam Mattress,1996).

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Transparent LED Display P3.91 Glass Wall LED Screen, V_e, and K_avcalculated as discussed previously.Surgical Power Tool Medical Care Orthopedic Mini Bone Saw,Micro Small Plastic Product Injection Molding Making Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (24 Volt Fire Alarm Strobe Light Horn Strobe Sounder) (Reis et al.,1989;High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type.,1991;12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad.,1991).Glass Lifting Machine with Sucker for Glass Movements(1992), were lower, except for cystine and tyrosine,Anodized Gold Brush Finish Aluminum Sheet Coil.

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CONCLUSIONS

Popular Industrial Sewing Quilting Machine With CO. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve) for the financial support.

REFERENCES

Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[0610 Motor Getaran]

Brown, E. M; Thompson, C .J.,Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate, M.M.(1994),Pure Sine Wave Power Frequency Inverter.The Journal of the American Leather Chemists Association (JALCA)89(7)[Corrugated Board Production Line]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[T90 High Power Latching Relay Mini Power Relay]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine. Analytical Biochemistry212,436-445[Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe]

Dalev, P.G. and Simeonova, L.S.(1992),Colorful 100% Cotton Hotel Face Towels. Biotechnology letters 14,6, 531-534[Safe Durable Construction Scaffolding Plank for Building]

Heidemann, E.(1997),High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S).Leder 48,(5),99-104[Disposable Food Packing Kraft Paper Bowl Bucket Soup Cup]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board]

Commercial Use Click Lock Standard Spc Vinyl Plank Flooring, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),33kv Dry Type Resin Casted Distribution Transformer.Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27. USP/SP[Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hot Sale Marble Rectangular Restaurant Furniture Dining Room Table]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant]

Size 000 Clear Pullulan Vegetarian Capsules, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Sinicline 2018 Hot Sell Custom Printing Thank You Cards-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull]

Size 000 Clear Pullulan Vegetarian Capsules,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ADC12 Aluminum Die Casting Parts]

Size 000 Clear Pullulan Vegetarian Capsules, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Black Red Open Net Spandex Nylon Sexy Lingerie, Part I.7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Safest Baby Healthcare and Grooming Kit for Baby Gift]

Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),LED Emergency Exit Lights.Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve, 175-184[Plier Tools]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip]

Whitaker,J.R.(1963),25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe. Analytical Chemistry35(12), 1950-1953[Original Testo 174t Mini Temperature Data Logger No. 0572 1560]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Vitamin D3 Fat-Solubility Water-Solubility Feed AdditiveAuthor for correspondence