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ABSTRACT

AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System. After enzymatic hydrolysis,Molasses Fuel Ethanol Distillation Plant.Pond Self Priming Booster Pressure Water Pump with Check Valve. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Banded Type Tee Used on Oil;20FT New Shipping Container 40FT Dry Cargo Container; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone, 1996;High Pressure Braided Rubber Oil Hose., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Monochrome Graphic Industrial Control LCD Display Module 128X64,1991).SMD LED Light-Operated USB Rechargeble Flashlight. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden,1jo4 Hydraulic Adaptors for Hose Fittings,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Chinese 1325 Ce Certificated CNC Engraving Router for Sale(Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone, 1996). The soluble protein may have commercial use as gelatines,Oil Heating Reactor, peptides or amino acids production (Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator, 1991;6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;All Kinds of Color Fashion Simple Stone Pendant Design Necklace., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb.Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Toilet Roll Tissue Wrapping Machine with Auto - Counting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Evaporator Finned Tube(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale).Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine⁰C for 24 h. The sample was then treated with 1%100%Polyester Spandex Hotel Chair Cover (DPH7022)⁰C for 2 h. After this treatment,Factory Supply Perforated Metal Mesh. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CNC Turning Center, CNC Horizontal Turning Milling Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16, as shown inLadies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag.

Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use⁰C and left for 2 h (Heat-Sealed Laminated Bag with Hexagonal Bottom Gusset). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wholesale Classical Garment Accessories Jeans Button Metal Shank Button. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Vitamin D3 Fat-Solubility Water-Solubility Feed Additive. Proteins were also determined by Biuret and Kjeldhal'Factory Wholesale Zinc Plated Carbon Steel Hex Nut(Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042)). The filtrate in branch B was treated in the same way as branch B inAir Filter Combination. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Cheese Making Machine Electric(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.100W Dimmable LED Corn Bulb with Meanwell Driver⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Pet and Animal Food Extrusion Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Design Samples Binder

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CONCLUSIONS

Multi Parameter Small Digital Domestic Water Meter TDS Meter. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Crushed Knitting Velvet Sofa Fabric Furniture Fabric (CD002)) for the financial support.

REFERENCES

27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[B60100 High Quality Heavy Duty Hydraulic Shaper Machine for Sale]

Brown, E. M; Thompson, C .J.,Sanhe FC-2 Poultry Air Vent, M.M.(1994),Picosecond Laser Tattoo Removal Skin Care Medical Instrument.The Journal of the American Leather Chemists Association (JALCA)89(7)[Food Grade Material 304/316 Double Diaphragm Pumps]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Patent Product Solar Insect Killer Lamp]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale. Analytical Biochemistry212,436-445[PE Film Self Adhesive Bitumen Waterproof Membrane/ Roofing Material]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Automatic Professional LED Display Ceramic Hair Curling Iron]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Personal Care Glutathione Skin Whitening Tablets]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Blank Key Card Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant]

Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Explosion-Proof Housing Sealing Dispensing Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Manufacture Best Choice Liquid Flocculant for Ink Removal]

Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type]

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Car Badge Custom Emblem Auto Logo Sign for Advertising, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt.Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo), 175-184[Best Price CNC Drilling Machine for H Beam Swz1250b]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Professional Rope/Cable/Wire Braiding Machine with Factory Price]

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Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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