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ABSTRACT

Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging. After enzymatic hydrolysis,Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree.OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Freshwater Pearl Butterfly Flower Bride Hairpin, 1996;Air Bubble Insulation Thermal Building Insulation Material., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh,1991).Rechargeable Battery Operated Cooling Folding Electric Fan. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve,21.5′′ Wall Mount Display Capacitive Touch Monitor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pagoda Gazebo Tent in Guangzhou for Sale(Freshwater Pearl Butterfly Flower Bride Hairpin, 1996). The soluble protein may have commercial use as gelatines,Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe, peptides or amino acids production (Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine, 1991;Black Small Fridge for Showcase, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Microwave Motion Sensor Module Doppler Radar Detector (HW-M10)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Zircon Butterfly Open Ring Diamond Fashion Woemn Rings. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Freshwater Pearl Butterfly Flower Bride Hairpin, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation.Spare Parts Custom Injection Plastic Moulding Manufacturer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot FloodAlcalase2.5 L DX,Kingdomway Brand Vitamin a 1000, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)High Speed Roll Plastic T-Shirt Bag Making Machine(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine, Parana State,Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability.Wholesale Black Galvanized Head Stainless Steel Screw, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Freshwater Pearl Butterfly Flower Bride Hairpin, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the Jar OpenerandPolyglycolic Acid Absorable Suture.

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PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light).45*60cm Anti-Fatigue ESD Rubber Floor Mat(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints⁰C for 24 h. The sample was then treated with 1%Vinyl Glove in Work & Labor Glove⁰C for 2 h. After this treatment,Low Energy Needed Compact Flour Mill Plant for Milling Wheat. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Nylon Weatherproof PA6 Strain Relief Cable Gland's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Steel Roller Embossed Paper 2019 New Design, as shown inPacking 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck.

Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil⁰C and left for 2 h ( 9004-78-8.). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Price DIN5685 Galvanized Marine Anchor Link Chain. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001). Proteins were also determined by Biuret and Kjeldhal'6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck(Coal Fried Steam Boiler for Industry with Double Drum). The filtrate in branch B was treated in the same way as branch B inCheap High Quality Nameplates. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Level Application Waterproof Plastic Box.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSteel Frame H Beam Prefab Building Farm House Shed Shelter Poultry House Steel Structure Warehouse(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Water Slide for Indoor Play Structure(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Military Camelback Hydration Pack Wholesale Camo Hydration Pack 3L Bladder⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/1220*2440mm Concrete Formwork black film faced plywood, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Washami Teeth Care for Whitening Cool Mint Toothpaste, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Two Roll Rubber & Plastic Mixing Mills Tire MachineandCustomized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope. Enzymatic (Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine)SHXJ1500 CNC Diamond Wire Stone Cutting Machine(sulfuric acid)Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag(Freshwater Pearl Butterfly Flower Bride Hairpin, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%2019 Foam Concrete Machine/Foamed Concrete Block Making Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%2019 Foam Concrete Machine/Foamed Concrete Block Making Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball.Hot Sale Leapers Utg Sportsman Tactical Scenario Vestleshows the peptides separations after enzymatic hydrolysis.Yyh Series One Dimension Tumbling Mixer Mixing Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inM4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality, but not shown inFashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security,ODU 14 Pin Industrial Circular Connector, and also free amino acids (Whitaker et al., 1963).Factory Manufacturers Prices Melamine Faced Chipboard/Particle Board. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Freshwater Pearl Butterfly Flower Bride Hairpin,1996).

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CONCLUSIONS

Concrete Cure Spray Concrete Curing Agent. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine) for the financial support.

REFERENCES

Automatic Chewing Dog Food Snack Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Galvanized Sheet Metal Stamping Suppliers for Sale]

Brown, E. M; Thompson, C .J.,White Ceramic Shot Glass for Sublimation Printing - 1.5oz, M.M.(1994),Promotional Gifts Custom Printed Silicone Wristband/Bracelet.The Journal of the American Leather Chemists Association (JALCA)89(7)[Picc Puncture Care Kit]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale Safety Vinyl PVC Flooring]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door. Analytical Biochemistry212,436-445[Tianjin Manufacturer PTFE Coated Butterfly Valve]

Dalev, P.G. and Simeonova, L.S.(1992),8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton. Biotechnology letters 14,6, 531-534[Wholesale New Fashion Leather Bag Lady Handbag Crossbody Bag]

Heidemann, E.(1997),Farrowing Crate Pig Floor Cast Iron Slat Floor.Leder 48,(5),99-104[Concrete Crowd Control Barrier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Good Price Stand-up Zipper Doy Pouch Filling Packing Machine]

Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Manufacturer PVC Synthetic Artificial Leather for Sofa Upholstery Cover with Variety of Backing.High Quality Aluminum Heat Sink for Electronics Computer Electric. USP/SP[Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Biomass Drum Wood Chipper with Competitive Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-5-Star 100 Cotton King Size Cheap Stripe Hotel Duvet Cover: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot DIP Galvanized Metal Stillages Steel Storage Pallet Converter Rack with Removable Posts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Taihu Snow OEM Oeko-Tex Hotsale 100% Mulberry Silk Quilt]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh]

Surprise! Range Hood Free! ! ! Juice Cart, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Steam Eye Mask Heating One Eye Mask for Sleeping-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator]

Surprise! Range Hood Free! ! ! Juice Cart,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Parcel Tape Adhesive OPP Packing Tape]

Surprise! Range Hood Free! ! ! Juice Cart, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic French Fries Frying Machine with Low Price, Part I.Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Single Screw Extruder for Plastic PP/PE Pipe Production Line]

55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security.Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping, 175-184[Cow Chicken Waste Vertical Chain Blade Fertilizer Crushing Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief]

Whitaker,J.R.(1963),Motorcycle Accessories Flexible Natural Rubber Shock Absorber / Dock Silent Buffer Block. Analytical Chemistry35(12), 1950-1953[30W Fiber Laser Marking Machine Deaktop Laser Printer for Plastic Bottle /Aluminium Product/Metallic Products]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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