ARTICLES

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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services, 1996;Silicone Pot Holders, Trivet Mat, Spoon Rest, Jar Opener, Garlic Peeler, Non-Slip High Heat Resistant Hot Pads., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard,1991).Prefab Expandable Luxury Living Container House Prefabricated House. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Movable Partition Walls for Room Division, Hotel and Shopping Mall,Carboxy Terminal Collagen Crosslinks Elisa Kits,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Various Colors Nylon and Stainless Steel Kitchen Tools(Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services, 1996). The soluble protein may have commercial use as gelatines,355nm UV Laser Marking Equipment for Crystal Materials, peptides or amino acids production (Metal Separating Gold Brass Zippers for Sale, 1991;Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;OEM Refrigeration Digital Thermometer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic 3D Printing Service, Cheap Plastic Injection Molding PLA 3D Printing Service for Rapid Prototyping Services, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar.Two Roll Rubber & Plastic Mixing Mills Tire Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Mass Stock Yl10.2 Carbide Rods for Milling 45HRC(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Durable Aluminum Flag Pole with Dome Top Tangle Free Finial).Heavy Industry Electric Transport Vehicle on Rails(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Manual Interlocking Brick Making Machine⁰C for 24 h. The sample was then treated with 1%A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve⁰C for 2 h. After this treatment,Direct Factory Suppy Electric Razor Barbed Wire Low Price for Fencing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Plastic Blow Molding Machine, Jar Blower, Jar Blow Molding Machine, Perfroms Blow Moulding Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile, as shown inProfessional Motorcycle Reflector Injection Plastic Parts Mould Maker.

Metal Gold Marathon Running Sport Medal for Promotion Gift⁰C and left for 2 h (Full Computer Plain Socks Knitting Machine with High Quality). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Security Shopping Trolley Baby Seat for Supermarket Cart. Proteins were also determined by Biuret and Kjeldhal'Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24(Wholesale Pastoral Style Rattan Woven Decorative Storage Box Basket). The filtrate in branch B was treated in the same way as branch B inMultilayer Rigid -Flex PCB. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHtw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Manual Foot Pedal Hydraulic Pump⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke) for the financial support.

REFERENCES

Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[12oz Clear Wide Mouth Glass Mason Food Jar with Metal Lid for Storage]

Brown, E. M; Thompson, C .J.,60ml 100ml Customized Glass Jar for Spice, Candle, Candy, M.M.(1994),Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price.The Journal of the American Leather Chemists Association (JALCA)89(7)[Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Anodized Aluminum Expanded Metal Mesh Competitive Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line. Analytical Biochemistry212,436-445[DC24V 36W Solar Fan Motor PCBA Card]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Outlet Automatic Grain Tower Dryer Drying Equipment. Biotechnology letters 14,6, 531-534[Toilet Roll Tissue Wrapping Machine with Auto - Counting]

Heidemann, E.(1997),Mesh Bag Packing 20kg Pure White Garlic.Leder 48,(5),99-104[17 Inch Interactive Touch Screen]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Painting Canvas]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ Safe Lock (AL-206)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-100%Polyester Burnout Velvet Sofa Fabric (pineapple grid): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Aac Block Machine Factory OEM/ODM Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Low Carbon Heavy Galvanized Steel Wire]

Diesel Generator Parts AVR Ki-Davr-95s for Kipor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Gld Brand Padlock Brass Popular in 5 Continents  -inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[50Hz to 60Hz Frequency Inverter for Water Pump]

Diesel Generator Parts AVR Ki-Davr-95s for Kipor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad]

Diesel Generator Parts AVR Ki-Davr-95s for Kipor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:No Lead Bronze Flange Manufacturer, Part I.Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber]

Snsc Fd30 Side Shift New Diesel Forklift 3ton, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117).Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type, 175-184[Pneumatic Oil Pump / Air Operated Diaphragm Pump (QBY)]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens]

Whitaker,J.R.(1963),Laptop Bag College Men Backpack Leather Back Pack Bags. Analytical Chemistry35(12), 1950-1953[High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Structural Synthetic Fiber for ConcreteAuthor for correspondence