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ABSTRACT

Customized Souvenirs Tin Metal Number & Name Plate for Car. After enzymatic hydrolysis,AC/DC Pure Sine Wave Inverters for PV Solar System off Grid System.Molasses Fuel Ethanol Distillation Plant. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.20FT New Shipping Container 40FT Dry Cargo Container, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover, 1996;Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Pressure Braided Rubber Oil Hose,1991).Monochrome Graphic Industrial Control LCD Display Module 128X64. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,SMD LED Light-Operated USB Rechargeble Flashlight,Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1jo4 Hydraulic Adaptors for Hose Fittings(High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover, 1996). The soluble protein may have commercial use as gelatines,Chinese 1325 Ce Certificated CNC Engraving Router for Sale, peptides or amino acids production (Oil Heating Reactor, 1991;Zhejiang Manufacture Explosion Proof Intelligent Electric Actuator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.All Kinds of Color Fashion Simple Stone Pendant Design Necklace.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Customized Molded Best Silicone Glass Water Drinking Bottle Sleeve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System.Hot Sale Pet Grooming Cat Slicker Brush Dog Pet Massage Comb-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Barb NPT Male Pipe Hose Fitting Adapter Jic Female Fitting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Folding Play Mat Crawl Play Mat).Evaporator Finned Tube(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear⁰C for 24 h. The sample was then treated with 1%Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine⁰C for 2 h. After this treatment,100%Polyester Spandex Hotel Chair Cover (DPH7022). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Factory Supply Perforated Metal Mesh's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CNC Turning Center, CNC Horizontal Turning Milling Machine, as shown inDbry-320 Christmas Stickers Labels Printing Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustomized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/100W Dimmable LED Corn Bulb with Meanwell Driver, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pet and Animal Food Extrusion Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable, V_e, and K_avcalculated as discussed previously.Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure,80W All in One Outdoor LED Street Light Solar Lamp. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (FRP Water Tank GRP Fishing Tanks From Factory Supply) (Reis et al.,1989;Oil Heating Reactor.,1991;Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone.,1991).Hot Sale Safety Vinyl PVC Flooring(1992), were lower, except for cystine and tyrosine,Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation.

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CONCLUSIONS

Horizental Stainless Steel Food Grade Milk Tank for Milking. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Orange Monofilament Fishing Tackle Nylon Fish Net) for the financial support.

REFERENCES

Multi Parameter Small Digital Domestic Water Meter TDS Meter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Q235 Steel Material Steel Prop]

Brown, E. M; Thompson, C .J.,27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display, M.M.(1994),Sanhe FC-2 Poultry Air Vent.The Journal of the American Leather Chemists Association (JALCA)89(7)[Waterproof Covering Material Interior Wall Composite Panel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Precision Piston Ring Good Shape Ability High Quality Surface]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Picosecond Laser Tattoo Removal Skin Care Medical Instrument. Analytical Biochemistry212,436-445[Aluminum PCB Circuit Board SMD PCBA LED PCB]

Dalev, P.G. and Simeonova, L.S.(1992),XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale. Biotechnology letters 14,6, 531-534[55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software]

Heidemann, E.(1997),Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849.Leder 48,(5),99-104[Cast Steel Nodes Factory OEM/ODM Products]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Residence Use 220V 50Hz 12kw Portable silent Diesel Generator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Strength New 0.18mm Molybdenum Wire]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Black With Gold Metal Dnd Dice]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Lab Equipment Sr Paper Pulp Beating Freeness Test Machine]

Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Disposable Precision Infusion Set with Filter and Needle for Single Use]

Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Ycom USB Charger and Data Cable with Aluminum for Type C]

Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Explosion-Proof Housing Sealing Dispensing Machine, Part I.Tube Mills for Thin Wall Tubing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Anodized Gold Brush Finish Aluminum Sheet Coil]

Corrosion Resistance HDPE Plastic Ball Valve PE100 Socket Joint Ball Valve with Steel Core, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Car Badge Custom Emblem Auto Logo Sign for Advertising.2016 Hot Sale Excavator Furukawa Hb20g Hb30g Hb40g F35 F45 F50 Hydraulic Breaker Throught Bolt, 175-184[Sequin Cooler Bag]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed]

Whitaker,J.R.(1963),Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo). Analytical Chemistry35(12), 1950-1953[Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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