ARTICLES

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ABSTRACT

GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable. After enzymatic hydrolysis,Hairs Comb for Pet Dog Cats.Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine;Australia C-Tick Testing and Certification Service for Electric Scooter; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (35 Degree Escalator with Auto Start Function in Parallel, 1996;Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bus Diesel Engine Parts 1105-00058 Fuel Filter for Yutong Zk6129h,1991).Roller Wedge Screen Cylinder. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup,Push Pull Small Cable to Cable Connector,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Stainless Steel Seamless Pipe ASTM A312 TP304 316(35 Degree Escalator with Auto Start Function in Parallel, 1996). The soluble protein may have commercial use as gelatines,Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco, peptides or amino acids production (Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars, 1991;Big Chocolate Storage Holding Tank, Chocolate Buffer Tank, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Asco 353 Series 3/4 Pulse Valve Solenoid Valve.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (35 Degree Escalator with Auto Start Function in Parallel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle.Purple UL 1095 16AWG Electronic Lead Wire-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Brushed Anodizing Aluminum Extrusion Profile for LED(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle CapsAlcalase2.5 L DX,S235jo S235jr Steel Pipe Tube Shs Rhs, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Pre-Seasoned Cast Iron Mini Oval Casserole Pot(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Stainless Steel Square Wire Mesh Netting with SGS, Parana State,Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware.Europe Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (35 Degree Escalator with Auto Start Function in Parallel, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theElectric Heavy Duty Rail Guided Vehicle for TransportationandDesktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24.

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Solar and Industrial Homehold Sine Wave Smart Power Inverter100W 500W -6000W DC12V-DC60V to 220V Output with Digital Display(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel).Outdoor Garden Save Energy with Solar Flame Wall Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)⁰C for 24 h. The sample was then treated with 1%Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag⁰C for 2 h. After this treatment,Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Non Stick Silicone Coated Fiberglass Cloth's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502, as shown in Laser Cutting Ceramic.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Sale 100% Polyester Cartoon Kids Sleepwear. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCe Quickclean Hyaluronic Acid Injections for Osteoarthritis Pain(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Laparoscopy Clip Applicator for Abdominal Surgery Uses⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Yield Tree Stump Crusher Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Diaphragm Valve for Bag Filter Since 1992, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Walland4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf. Enzymatic (Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine)Manufacturer Plastic Injection Mould Molding Nylon PP Injection(sulfuric acid)Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe(35 Degree Escalator with Auto Start Function in Parallel, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack, flow rate of 0.1 mL/min., and l = 620 nm.

Aluminum Forging Cast Metal Sand Die Casting.Good Quality White PVC Rigid Foam Board for Iran Marketshows the peptides separations after enzymatic hydrolysis.ADC12 Aluminum Die Casting Parts(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMolasses Fuel Ethanol Distillation Plant, but not shown inCentral Loose Tube No Armored 2 - 24 Core Fiber Optic Cable,Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale, and also free amino acids (Whitaker et al., 1963).Custom Logo Cotton Terry Oversize Pullover Men Hoodie. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (35 Degree Escalator with Auto Start Function in Parallel,1996).

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Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW)andColumn Type Pave Rubber Tile Making Machine-Vulcanizing Press Machineshow the gel chromatographic separation of soluble protein with Sephadex G-Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug.Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer, and another peak at 45 mL not shown inNon Stick Silicone Coated Fiberglass Cloth, for peptides lower than 3000 Da.Seikofire S-66 Palm OTDR Sm/mm Fiber Testing(Condell et al.,1993) .

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PVC Fabric Rapid Roller Shutter for Clearn Room, V_e, and K_avcalculated as discussed previously.Fiberglass Animal Poultry Insulation Grain Tower,Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Mold Injection Molding Plastic Products White PC Housing) (Reis et al.,1989;Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars.,1991;Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings.,1991).Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights(1992), were lower, except for cystine and tyrosine,Wholesale 375ml Dark Green Glass Ice Wine Bottles.

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CONCLUSIONS

Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing) for the financial support.

REFERENCES

Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[12kv 15kv 20kv 33kv 35kv Indoor and Outdoor Mv Current Transformer]

Brown, E. M; Thompson, C .J.,Cog Graphic FPC LCD Display Module, M.M.(1994),Power Fitting- Hot DIP Galvanizing Bolt with Nut.The Journal of the American Leather Chemists Association (JALCA)89(7)[500 Tons Hydraulic Power Double Action Press with Cushion]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Sanhe FC-2 Poultry Air Vent]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Slewing Gear for Tower Crane Slewing Winch Reducer. Analytical Biochemistry212,436-445[China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board]

Dalev, P.G. and Simeonova, L.S.(1992),Full Automatic Tomato Ketchup Sauce Sachet Packaging Machine. Biotechnology letters 14,6, 531-534[Farm Relief Plastic Automatic Air Release Valve]

Heidemann, E.(1997),7-Gauge Polyester/Cotton Knitted Gloves with PVC DOT on One Side.Leder 48,(5),99-104[Diamond Floor Preparation Tools]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Kh No Fried Automatic Potato Chips Making Machines]

High Quality Stainless Steel Expanded Metal Mesh, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Shoes Sandals Sneakers Comfortable Foam Injection Moulded Shoes.Double Arm Motorized Multifunction Medical Equipment for Hospital Gas System. USP/SP[Solar Energy Storad Power Generation System]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PP Needlepunched Nonwoven Geotextile]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[gzyezhifurniture]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Modern Office Ergonomic Executive Leather Swivel Office Chair (HC-Venlo)]

Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Patent LED Tri-Proof light Fixture 30W, 3FT 3000lm IP66 Approved-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electrical Stationary Hoist Tower Crane 500kg Motor]

Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[8000kg Diesel Forklift Great Logistic Equipment Fork Lift Truck 8ton]

Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Single Screw Extruder for Plastic PP/PE Pipe Production Line, Part I.Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[FM USB Radio Audio Power Amplifier for Home Bar Club]

Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Gold Star Square Glass Candle Holder for Wedding Decoration.11kg 24L Low Pressure LPG Gas Cylinder, 175-184[Online Buy Natural Arnica Montana Extract 10: 1 Raw Powder]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Yashi Touch Screen Smart Interactive Whiteboard for Office]

Whitaker,J.R.(1963),Commercial Use Click Lock Standard Spc Vinyl Plank Flooring. Analytical Chemistry35(12), 1950-1953[Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hydrophobic Excellent Thermal Insulation Silica Aerogel PowderAuthor for correspondence