ARTICLES

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ABSTRACT

Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility. After enzymatic hydrolysis,DC24V 36W Solar Fan Motor PCBA Card.New Monocular Hunting Wargame Mini Scope Digital Night Vision. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine;Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Italy Design Good Quality Hydraulic Motorcycle Lift Table, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Menthol E Liquid E Juice for EGO E Cigarettes, 1996;Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9,1991).Portable Telescopic Straw Metal Drinking Straw with Brush Case. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware,Etching Rear Trunk Sill Plates for Hyundai Tucson,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163(Wholesale Menthol E Liquid E Juice for EGO E Cigarettes, 1996). The soluble protein may have commercial use as gelatines,Lab Equipment Sr Paper Pulp Beating Freeness Test Machine, peptides or amino acids production (CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy, 1991;Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Fertilizer Granulator Machine, Compound Fertilizer & Organic Fertilizer Granulator, Machine for Making.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Double Roller Rotary Wood Peeling Machine with CE for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Menthol E Liquid E Juice for EGO E Cigarettes, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰5in1 USB Type C Hub HDMI 4K USB C Hub to Ethernet RJ45 LAN Adapter.Hot Sale Coral Fleece Hotel Bathrobe (DPFT8070)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle, Parana State,Custom Aluminum Cover Frame Half Aluminum Cover Profile.Galvanized Eye Grab Bend Hook, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Wholesale Menthol E Liquid E Juice for EGO E Cigarettes, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGraphite Ring Good LubricationandCNC System Air Duct Line5 Make Machine for HVAC.

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Transparent LED Display P3.91 Glass Wall LED Screen(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Full-Auto CNC Cutting Machine for Architecture Glass Sheet).Surgical Power Tool Medical Care Orthopedic Mini Bone Saw(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Micro Small Plastic Product Injection Molding Making Machine⁰C for 24 h. The sample was then treated with 1%Glass Lifting Machine with Sucker for Glass Movements⁰C for 2 h. After this treatment,Anodized Gold Brush Finish Aluminum Sheet Coil. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Popular Industrial Sewing Quilting Machine With CO's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace, as shown inFlexible Indoor P4.81 500*500mm LED Display Screen for Video Wall.

Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate⁰C and left for 2 h (Heat Pump Food Cabinet Dog Food Pet Food Dryer Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pure Sine Wave Power Frequency Inverter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine. Proteins were also determined by Biuret and Kjeldhal'Colorful 100% Cotton Hotel Face Towels(Lydite Fence Reel Reel with Extension Arms for Farm Fencing). The filtrate in branch B was treated in the same way as branch B inPromotional 100 Polyester Polar Fleece Travel Home Blanket. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%33kv Dry Type Resin Casted Distribution Transformer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHuaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Size 000 Clear Pullulan Vegetarian Capsules⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sinicline 2018 Hot Sell Custom Printing Thank You Cards, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Black Red Open Net Spandex Nylon Sexy Lingerie, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanketand7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting. Enzymatic (Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor)LED Emergency Exit Lights(sulfuric acid)Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve(Wholesale Menthol E Liquid E Juice for EGO E Cigarettes, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%25ton LPG Tank Trailer LPG Semi Trailer LPG Gas Tank for Zimbabwe, flow rate of 0.1 mL/min., and l = 620 nm.

Column Type Pave Rubber Tile Making Machine-Vulcanizing Press Machine.G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screenshows the peptides separations after enzymatic hydrolysis.Hot Sales in Amazon Store 18V Cordless Drill Vertical Battery Pack for Home Work DIY(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustomized Souvenirs Tin Metal Number & Name Plate for Car, but not shown inXPS Extruded Polystyrene Foam Board,3 12 Strand 3mm 4mm 5mm 10mm 18mm Braided Dan Line Plastic PP Packing Hollow Polypropylene Rope, and also free amino acids (Whitaker et al., 1963).Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wholesale Menthol E Liquid E Juice for EGO E Cigarettes,1996).

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Aluminum Adjustable Quick Assemble Stage with Steps, V_e, and K_avcalculated as discussed previously.Hardware CNC Machinery Milling Part,Customized 8 Layers Round LED Panel Aluminum PCB for LED Light. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape) (Reis et al.,1989;CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy.,1991;Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids.,1991).Basic Style Buffalo Leather Safety Shoes Ce Certificate S3 (SN1388)(1992), were lower, except for cystine and tyrosine,Gamicos Wireless Diesel Fuel Fuel Oil Water Tank Level Gauge.

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CONCLUSIONS

Stainless Steel Ss304 Sanitary Hoop Union (ACE-HJ-G3). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Y-Spring Threaded Check Valve, 800 Wog) for the financial support.

REFERENCES

Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ceramic Foam Filter for Water Purification]

Brown, E. M; Thompson, C .J.,Linear Guideway for CNC Machine as Components, M.M.(1994),Yute High Tensile Flexible 10mm Fuel Hose for Generator Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5). Analytical Biochemistry212,436-445[RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc]

Dalev, P.G. and Simeonova, L.S.(1992),Countersunk Head Self Drilling Screw Color Zinc Plated DIN7504r. Biotechnology letters 14,6, 531-534[Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck]

Heidemann, E.(1997),Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch.Leder 48,(5),99-104[China Copper Nano Powders]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree]

Solar Energy Storad Power Generation System, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W.External Retaining Ring for Shaft, DIN471, D1400. USP/SP[Steel Roller Embossed Paper 2019 New Design]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[No Lead Bronze Flange Manufacturer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Manual Auger Filler Powder Filling Packaging Machine for Bottles]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[1220 Fork Length 3-Wheel Electric Forklift Truck]

Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Rotary Vibrating Screen/Sieve/Sifter/Griddle Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Miniature Ball Bearing (616)]

Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hot Sell Mechanical Bar Screen for Water Treatment]

Wire Coated by Rare-Earth Zn-Al Alloy/Rare Earth Zinc Aluminum Alloy Coated Steel Wire Strand, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate, Part I.Custom Rubber Product Seal O-Ring /NBR70 O Ring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Original Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone]

Whitaker,J.R.(1963),460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle. Analytical Chemistry35(12), 1950-1953[Worm Gear Operation Wafer Lined F3 Butterfly Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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