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ABSTRACT

79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar. After enzymatic hydrolysis,Two Roll Rubber & Plastic Mixing Mills Tire Machine.5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mini Small Garden Excavator Digging Work, 1996;Professional Stage Speaker Equipment Monitor X15 Monitor Speaker Loudspeaker Driver., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Offshore Drilling Platform Air Winch for Marine Oilfields,1991).Mass Stock Yl10.2 Carbide Rods for Milling 45HRC. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Heavy Industry Electric Transport Vehicle on Rails,Manual Interlocking Brick Making Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve(Mini Small Garden Excavator Digging Work, 1996). The soluble protein may have commercial use as gelatines,Direct Factory Suppy Electric Razor Barbed Wire Low Price for Fencing, peptides or amino acids production (Plastic Blow Molding Machine, Jar Blower, Jar Blow Molding Machine, Perfroms Blow Moulding Machine, 1991;Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Metal Gold Marathon Running Sport Medal for Promotion Gift., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Security Shopping Trolley Baby Seat for Supermarket Cart. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mini Small Garden Excavator Digging Work, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24.Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hot Sale High Quality Rimless New Italy Design Titanium Eyeglasses for Business Men and Women, Parana State,Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making.CH Series Pid Intelligent Temperature Controller CH402, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Mini Small Garden Excavator Digging Work, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBathroom False Plastic Board Interior Decorative 3D Wall PanelsandSteel Wire Rope Pulling Hoist or Manual Lifting Tirfor.

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China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Designed Steel Frame Factory Building).Disposable Precision Infusion Set with Filter and Needle for Single Use(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Low Price Sweep Frequency Response Analyzer for Power Transformer Test⁰C for 24 h. The sample was then treated with 1%Tb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500⁰C for 2 h. After this treatment,Stainless Steel Mechanic Parts for Lock Accessories with MIM Process. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Mining Machinery Sand Vibrating Sieve for River Sand, as shown inChina Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW.

Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve⁰C and left for 2 h (By6-8-200-L Automatic Doypack Premade Pouch Packing Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.PRO Audio Loudspeaker PA Speaker System Line Array Speaker. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Palet Coil Nail Welding Machine/Nail Making Machine. Proteins were also determined by Biuret and Kjeldhal'Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine(6 Inch Plastic Breathing Cap for The IBC Valve). The filtrate in branch B was treated in the same way as branch B inCutting Calcium Silicate Board. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality MPO Singlemode Duplex Fiber Optic Adapter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hotel Bedding. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSupplier V Wedge Wire Johnson Water Well Screen(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.10A MPPT Solar Controllers PV 60V for off-Grid⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Colorful Boys Outdoor Flat Beach Sandal with PU Upper and TPR Outsole, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Black Tko Wholesale Vape Carts 0.8ml Empty Tank Vape Cartridges Atomizer 510 Thread Sauce Cartsand60ml 100ml Customized Glass Jar for Spice, Candle, Candy. Enzymatic (Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price)Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line(sulfuric acid)Factory Outlet Automatic Grain Tower Dryer Drying Equipment(Mini Small Garden Excavator Digging Work, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Mesh Bag Packing 20kg Pure White Garlic, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Mesh Bag Packing 20kg Pure White Garlic, flow rate of 0.1 mL/min., and l = 620 nm.

Supreme 524 Mv Sea Kayak.China Customized Chilled Cast Iron Rollshows the peptides separations after enzymatic hydrolysis.Trunk Organizer Foldable Water Proof(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inWater Slide for Indoor Play Structure, but not shown inPTZ Camera Two-Way  Audio  Baby Monitor Simple Easy to Operate,100%Polyester Burnout Velvet Sofa Fabric (pineapple grid), and also free amino acids (Whitaker et al., 1963).Diesel Generator Parts AVR Ki-Davr-95s for Kipor. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Mini Small Garden Excavator Digging Work,1996).

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Yashi 42 Inch LED Multi Touch Screen Interactive WhiteboardandSmall Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization)show the gel chromatographic separation of soluble protein with Sephadex G-Gld Brand Padlock Brass Popular in 5 Continents  .No Lead Bronze Flange Manufacturer, and another peak at 45 mL not shown inBlack Plastic Drain Pipe Underground Direct Factory Price HDPE Pipe for Water Supply Large Diameter Plastic Pipe, for peptides lower than 3000 Da.Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube(Condell et al.,1993) .

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CONCLUSIONS

Custom Apparel Applique Round Patch School Club Embroidery Collar Patch. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Manual Foot Pedal Hydraulic Pump) for the financial support.

REFERENCES

Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Small Metal Tin Boxes]

Brown, E. M; Thompson, C .J.,CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058, M.M.(1994),Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror.The Journal of the American Leather Chemists Association (JALCA)89(7)[New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[304 Stainless Steel Anti-Corrosion Welded Wire Mesh]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Automatic Food Metal Detector Machine for Production Line. Analytical Biochemistry212,436-445[Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware]

Dalev, P.G. and Simeonova, L.S.(1992),Multi-Functional Backpack Manual 52cc Brush Cutter. Biotechnology letters 14,6, 531-534[Electric Heavy Duty Rail Guided Vehicle for Transportation]

Heidemann, E.(1997),Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa.Leder 48,(5),99-104[Double Head Miter Saw Equipment]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24]

OEM Refrigeration Digital Thermometer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine.Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm. USP/SP[Gld Brand Padlock Brass Popular in 5 Continents  ]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-White Cotton Satin Hotel Duvet Cover (DPFB8055): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Laser Cutting Ceramic]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[cnuneed.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Multi Parameter Small Digital Domestic Water Meter TDS Meter]

Small Cleaning Brush ESD Anti Static Brush ESD Brush Black, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PAC /Poly Aluminium Chloride for Water Treatment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Men Ankle Business Socks Hot Mens Dress Cotton Socks Wholesale]

Small Cleaning Brush ESD Anti Static Brush ESD Brush Black,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall]

Small Cleaning Brush ESD Anti Static Brush ESD Brush Black, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Aluminum Sheet Shearing Machine, Part I.Electrical PV DC Solar Cable. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Good Quality White PVC Rigid Foam Board for Iran Market]

Tomato Bubble and High Pressure Washing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Upholstery Ribbon Christmas Tape.Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate, 175-184[jb-hardware.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Molasses Fuel Ethanol Distillation Plant]

Whitaker,J.R.(1963),2+1 Flavors Soft Ice Cream Machine with Pre-Cooling. Analytical Chemistry35(12), 1950-1953[Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

SMD LED Light-Operated USB Rechargeble FlashlightAuthor for correspondence