ARTICLES

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ABSTRACT

Muslim Hijab/Islamic Scarf Fashion Hijab Muslim Scarf. After enzymatic hydrolysis,460ml Glassware/Wine /Alcohol/Spirits/Glass Vodka Bottle.Ladies Handbag Straw Bag Promotion Crossbody Bag New Fashion Bag Women Leisure Shoulder Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;New Style Welded Tube Fittings Metric Male O-Ring Tee Stainless Steel Tube Joints;DIN 7337 Aluminium Core Pulling Stainless Steel Rivet; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Professional Bb Trumpet (ATR6506), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines, 1996;3.5ton Chinese Engine Gasoline Forklift (FG35T)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Disposable Environmental Biodegradable Takeaway Box Fruit Salad Box with Cover,1991).Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Waterproof Covering Material Interior Wall Composite Panel,Tall/Large Cube Glass Terrarium Planter Gift Card Holder,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Venlo Type Flower Multi Span Commercial Glass Greenhouses Used(Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines, 1996). The soluble protein may have commercial use as gelatines,XPS Extruded Polystyrene Foam Board, peptides or amino acids production (Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture, 1991;Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Kh No Fried Automatic Potato Chips Making Machines., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Deep Hole Cylinder Boring Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Good Prize Solar PV Mounting Panel System for Ground. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Electric Heavy Duty Rail Guided Vehicle for Transportation.Sower Vacuum Emulsifying Machine for Cosmetic-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:ASTM A240 309s Stainless Steel Sheet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Single Embossed Buttons Membrane Switch with FPC Circuit, Parana State,3D Laser Inside Engraving Machinery Use Germany Technology with High Speed.3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSolar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanternsand80W All in One Outdoor LED Street Light Solar Lamp.

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Popular Laser Cutting Engraving Machine for Nonmetals(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Explosion-Proof Housing Sealing Dispensing Machine).Disposable Medical Ventilator Breathing Circuit Anesthesia Smoothbore Corrugated Expandable Circuit(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Workstation Glass Frame Extrusion Aluminum Profiles for Office Partitions⁰C for 24 h. The sample was then treated with 1%Large Slewing Rings Bearings for Wind Turbine Ring on Sale⁰C for 2 h. After this treatment,Rectangular 1000ml Meal Prep Bento Boxes Take out Catering Microwavable Deep Container. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Chemicals Flocculant Cationic Polymer Price's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Ycom USB Charger and Data Cable with Aluminum for Type C, as shown inEasy Operational Cup Cake Filling Machine.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Bamboo Tablet Stand Laptop Rack Bt-2212. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWholesale Custom Hot Sale Recycled Colored Children Board Printing Story Book(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Personal Care Glutathione Skin Whitening Tablets⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Customized Over Size Boys Hoodie with Embroidered, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Digital Printing Football Fans Flag, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Promotional Plastic Laminated Custom Shopping PP Woven Bag.Cheap Gabion Wire Mesh Boxshows the peptides separations after enzymatic hydrolysis.Metal Galvanized Wave Form Perforated Steel Strips(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHidrotek Portable Suitcase Design Reverse Osmosis Demo Kits, but not shown inVarious Sublimation Blank Home Supply Fabric Bath Towel etc,Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12), and also free amino acids (Whitaker et al., 1963).Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Precision Stainless Steel Lost Wax Investment Casting Parts for Agricultural Machines,1996).

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CONCLUSIONS

Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (24K Gold EGF Serum Capsule Anti-Wrinkle Repairing Anti Aging Serum) for the financial support.

REFERENCES

Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[En471 Reflective Fabric Tape Cintas Reflectantes De Tela Plateada]

Brown, E. M; Thompson, C .J.,Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service, M.M.(1994),Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer.The Journal of the American Leather Chemists Association (JALCA)89(7)[110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[10A MPPT Solar Controllers PV 60V for off-Grid]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail. Analytical Biochemistry212,436-445[FTTH Fiber Optic Fusion Splicing Toolkit]

Dalev, P.G. and Simeonova, L.S.(1992),High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine. Biotechnology letters 14,6, 531-534[Safe Durable Scaffolding Trapdoor Plank for Construction]

Heidemann, E.(1997),Stainless Steel Clarifier Tank for Water Treatment.Leder 48,(5),99-104[shanghai linlang 100ml 200ml 250ml round glass reed diffuser bottles wholesale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Supplied High Strength Marine Anchor Chain]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Small Cleaning Brush ESD Anti Static Brush ESD Brush Black]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[New R&D Wire Cable Feeding Winding Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Carton Boxing Making Bottom Lock Folder Gluer Package Machinery]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line]

Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable]

Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Jh21 Series Pneumatic Power Press CNC Punching Machine]

Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002), Part I.2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer]

High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce.Dry Air Generator GF Series, 175-184[Boston Round Glass Bottle 1oz]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale]

Whitaker,J.R.(1963),Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture. Analytical Chemistry35(12), 1950-1953[Original Weichai Engine Truck Spare Parts Valve Cap 61500050105]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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