ARTICLES

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ABSTRACT

CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058. After enzymatic hydrolysis,Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror.Automatic Food Metal Detector Machine for Production Line. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Multi-Functional Backpack Manual 52cc Brush Cutter;Modern Living Room Furniture Hotel Bedroom Custom Corner Sofa; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Automatic Linear 5 Gallon Barrel Bottle Water Filling Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm, 1996;White Cotton Satin Hotel Duvet Cover (DPFB8055)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Small Cleaning Brush ESD Anti Static Brush ESD Brush Black,1991).PAC /Poly Aluminium Chloride for Water Treatment. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aluminum Sheet Shearing Machine,Electrical PV DC Solar Cable,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Tomato Bubble and High Pressure Washing Machine(Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm, 1996). The soluble protein may have commercial use as gelatines,Upholstery Ribbon Christmas Tape, peptides or amino acids production (Raggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor Concentrate, 1991;2+1 Flavors Soft Ice Cream Machine with Pre-Cooling, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Costa Rica Digital Smart Full HD ISDB-T TV Transmitter TV Receiver., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.40A Zinc Alloy Mini Plug-in Electronics Car Blade Fuse.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Best Sale Low Price Household Air freshener Spray. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohm, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Factory Supplied High Strength Marine Anchor Chain.Humidity Refrigeration Digital Controller Jsd-100+-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Elegant Europe Dessign Pillow Top Mattress(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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3D CNC Router for Wood Cutting Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08)).Stainless Steel Screens / Wedge Wire Filter Element(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Amazon Armor TPU Silicone Phone Case for Honor 10 Lite⁰C for 24 h. The sample was then treated with 1%EPS Foam Sheet Extrusion Machine for Fast Food Box Making⁰C for 2 h. After this treatment,Luxury High Quality Shawl Collar Multi-Color Coral Fleece Bathrobe. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Mobile Mini Self Loading Concrete Mixer Machines Production Line Js750ah's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)30PCS/M LED 7.2W 10 Pixels DC12V RGB DMX512 IP65 Strip Light, as shown inAluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure.

Wet Type Wire Drawing Machine for Carbon Steel and Stainless Steel⁰C and left for 2 h (Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.YAMAHA 60L Walk Behind Asphalt Road Crack Filling Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Stainless Steel Plate Flange. Proteins were also determined by Biuret and Kjeldhal'High Quality Low Price Top Selling Fashion Summer Boy Sandals(Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars). The filtrate in branch B was treated in the same way as branch B inExhaust Fan High Quality Supplier. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Easy Operational Cup Cake Filling Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Used Mini Excavator Komatsu PC55 PC40 PC35 PC15 PC18 Small Digger. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFolding Play Mat Crawl Play Mat(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Folding  Platform Hand  Trolley , pH 3.25., mobile phase B: buffer solution of 0.01 mol/Pump Flower Sprinkler with Automatic Watering System, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Plastic Caps Making Machine / Injection Molding MachineandFactory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chillershow the gel chromatographic separation of soluble protein with Sephadex G-High Quality CO2 Laser Marking Machine with Ce.New Design Inclined Plane 18W LED Wall Washer Lighting, and another peak at 45 mL not shown inLoquat Juice and Loquat Syrup Production Machinery Export Worldwide, for peptides lower than 3000 Da.2ton R Type Electric Wire Rope Hoist with Single Speed(Condell et al.,1993) .

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CONCLUSIONS

Hot Dipped Galvanized Oval Wire Brazil. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Modern 4 Person Workstation with Computer Desk Modular Office Furniture) for the financial support.

REFERENCES

Colorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Sinker Needle S-18-9-11]

Brown, E. M; Thompson, C .J.,Wood Grain Embossed Film for Flooring, Flower Design Film, Wood Grain Heat Transfer Polyester Film, Pet Heat Transfer Film, M.M.(1994),FDA ISO Ce OEM China Factory Hot Sale Custom Home Emergency Portable First Aid Kit.The Journal of the American Leather Chemists Association (JALCA)89(7)[Walking Long Distance Surveying Instrument Meter Measuring Wheel]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Micro DC 24V Worm Gear Brushless Motor for Medical Equipment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Custom Fashion Cup Mat Silicone Rubber Coaster for Tea- Best Housewarming Beverage, Drink Silicone PVC Coaster Beer Drink Coaster Cup Coaster (YB-CC-1). Analytical Biochemistry212,436-445[Carboxymethyl Cellulose CAS No. 9004-32-4]

Dalev, P.G. and Simeonova, L.S.(1992),Aluminum Foil PE Bottle Cap Induction Seal Liner. Biotechnology letters 14,6, 531-534[530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle]

Heidemann, E.(1997),Aluminium Profile/Shell/Housing for T5/T8 LED Tube Light (SQ-HRJ-002).Leder 48,(5),99-104[Stand Up Workstation]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Buy Mk 677 Powder Bulk Powders Sarms Mk 677]

Pet Dog Grooming Rake Dematting Tool Shedding Comb, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Sophisticated Durable Membrane Panel/Panel with 3m Glue.Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder. USP/SP[40ton Double Girder Low-Headroom European Electric Wire Rope Hoist/Winch (BMG-40122mm5)]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-HDPE Polyethylene PE Pipe for Water Supply: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Precision Low Deformation High Density High Pressure Resistance Complex Inner Cavity Smooth Surface Hydraulic Casting-Zm80 Motor Back Cover]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Double Head Silver/Black/Pink/Gold Dual Empty Eyeliner Tube]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service]

Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Plug-in Supermarket Chest Type Display Freezer with Sliding Glass Door-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Air Cooled Split Type Ducted Air Conditioner AC Unit]

Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Full Automatic 4000b/h 500ml Plastic Jar Making Machine]

Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station, Part I.Galvanized Pressure Locked Grating. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Made in China Lqa Strap Banding Tool]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application]

Whitaker,J.R.(1963),5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type. Analytical Chemistry35(12), 1950-1953[China Modern Pharmacy Vacuum Filter Press]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Folding Play Mat Crawl Play MatAuthor for correspondence