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ABSTRACT

Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce. After enzymatic hydrolysis,Dry Air Generator GF Series.Modern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Advanced Unlocking Tool for Locksmith;Screen Printing Materials and Equipments Screen Printing Frames; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Supplier Hard Ware Fittings Gas Spring for Tool Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price, 1996;Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet,1991).Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc,Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches(Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price, 1996). The soluble protein may have commercial use as gelatines,AISI 201/202/301/304 Stainless Steel Welded Square Pipe, peptides or amino acids production (Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce, 1991;Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.PVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Speed Copper Wire Braiding Making Machine.Safe Durable Construction Scaffolding Plank for Building-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Different Size of Ring NdFeB Magnet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Automatic Professional LED Display Ceramic Hair Curling Iron(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (110/220V Handle Mild Steel Welding Machine PCB Board 160A).CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer⁰C for 24 h. The sample was then treated with 1%Vulcanizing Machine /Rubber Tiles Vulcanzing Press⁰C for 2 h. After this treatment,Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Omni Newest Manager Desk Office Smart Office Table Office Staff Table, as shown inHardware CNC Machinery Milling Part.

6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap⁰C and left for 2 h (CT201795 Cartridge Chip for Xerox Docucentre IV 2056 2058). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage. Proteins were also determined by Biuret and Kjeldhal'Full-Auto CNC Cutting Machine for Architecture Glass Sheet(High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT). The filtrate in branch B was treated in the same way as branch B inProtocol Em4100/Em4102/Em4200/Em4550/Em4069/Em4305 Smart RFID PVC Sheet Card. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hot Forming Pushing Elbow Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.As568 Standard Brown FKM Rubber O Ring for Sealing⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Laundry Used Gas Water Heater with CE/Asme Certificateand200W UFO LED High Bay Light Fixture for Interior Exterior Applications. Enzymatic (China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2))13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder(sulfuric acid)Bathroom False Plastic Board Interior Decorative 3D Wall Panels(Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Mushroom Head Stud Sert, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Mushroom Head Stud Sert, flow rate of 0.1 mL/min., and l = 620 nm.

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Colorful  Reflective Sticker Reflective Tape, V_e, and K_avcalculated as discussed previously.Concrete Spare Part Electrical System Central Distribution Box,Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer) (Reis et al.,1989;Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce.,1991;Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed.,1991).50Hz to 60Hz Frequency Inverter for Water Pump(1992), were lower, except for cystine and tyrosine,Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330).

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CONCLUSIONS

High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Carboxymethyl Cellulose CAS No. 9004-32-4) for the financial support.

REFERENCES

4X8 Waterproof PVC Marble Sheet Wall Panels Decorative Bathroom Wall Panels, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Candle Boxes Packaging]

Brown, E. M; Thompson, C .J.,Vvvf Golden Mirror Sightseeing Passenger House Panoramic Home Observation Elevator, M.M.(1994),PVC Cabinet Construction Board, White 18mm PVC Foam Board.The Journal of the American Leather Chemists Association (JALCA)89(7)[Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2019 New Portable Mini Dental X-ray Machine. Analytical Biochemistry212,436-445[Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM]

Dalev, P.G. and Simeonova, L.S.(1992),Ss Reduced Port 2000wog 1PC Screwed Ball Valve. Biotechnology letters 14,6, 531-534[China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cable Tray with ′t′ Slots: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[F-Theta Lens Laser Marking Machine Spare Part]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts]

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10 Gauge Galvanized Welded Wire Mesh for Construction Using,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay]

10 Gauge Galvanized Welded Wire Mesh for Construction Using, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector, Part I.201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden]

Disposable PS Foam Fast Food Box Making Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A).Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP, 175-184[Slidable Rack Mount Type Patch Panel 12 Cores]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door]

Whitaker,J.R.(1963),Foska Good Quality Plastic Stapler Set with Staples. Analytical Chemistry35(12), 1950-1953[Mass Stock Yl10.2 Carbide Rods for Milling 45HRC]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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