ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Pharmaceutical Peptide Ghrp 2 Acetate CAS158861-67-7 Ghrp-2. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Big Capacity Crystal Clear Glass Wine Bottle with Screw Cap

ABSTRACT

Spare Parts for Caterpillar Construction Machinery. After enzymatic hydrolysis,Hpa Tank Refill Scuba Tank Air Fill Adapter Gas Transfer for Diving High Pressure Tank.50 Grams Ozone Producing Device for Makeup Toner Producing Plant. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cold Rolled Stainless Steel Strip /Coils 410/430/409, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (FRP Water Tank GRP Fishing Tanks From Factory Supply, 1996;Decorative House Stone Coated Metal Roof Tile., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig,1991).6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System,Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use(FRP Water Tank GRP Fishing Tanks From Factory Supply, 1996). The soluble protein may have commercial use as gelatines,Blue Steel Packing Strip, peptides or amino acids production (High Quality Powder and Wax Brush with Wooden Handle, 1991;Cheap Price Paper Straws Individually Wrapped Drinking Straw, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (FRP Water Tank GRP Fishing Tanks From Factory Supply, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Smelting and Heating Induction Machine (GW-HY187).Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Home Products 16′ Perforated Flex Pipe(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hot Sell Mechanical Bar Screen for Water Treatment, Parana State,Big Capacity Crystal Clear Glass Wine Bottle with Screw Cap.Glow Powder for Luminous Coating, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (FRP Water Tank GRP Fishing Tanks From Factory Supply, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSower Vacuum Emulsifying Machine for CosmeticandEfficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability.

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700 mm RP HP UHP Graphite Electrode Sold at Factory Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF).Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Union Metal Steel Building and Construction Material⁰C for 24 h. The sample was then treated with 1%Factory Directly Supply 10HP Air Cooled Water Chiller Best Price Industrial Chiller⁰C for 2 h. After this treatment,450ml Sublimation Stainless Steel Milk Bottle (Colorful). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Lower Noise Colled Rolled Ball Screw's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sphere Earth Permanent Magnets 216PCS 5mm Neodymium Cube Balls, as shown in48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System.

3.0kw Spindle CNC Router with Certificate for Woodworking⁰C and left for 2 h (Atlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.800W Electric Motor Price Lawn Mower Motor Concrete Mixer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Air Cooled Split Type Ducted Air Conditioner AC Unit. Proteins were also determined by Biuret and Kjeldhal'Hans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric(Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding). The filtrate in branch B was treated in the same way as branch B ingjmach-chn. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvet.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Gym Fitness Equipment Adjustable Weight Bench. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Sell P7.8 Transparent LED Display Screen(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Original Weichai Engine Truck Spare Parts Valve Cap 61500050105⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sliding Panel Saw Heavy Duty CNC Band Saw for Furniture Ban Resaw Machine for Lumber, Round Log Slinding Sawmill, Band Resaw for Lumber, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ceramic Edge Protection Profiles Decorative Ceramic Metal Tile Trim, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

304 Stainless Steel Anti-Corrosion Welded Wire Mesh. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (FM USB Radio Audio Power Amplifier for Home Bar Club) for the financial support.

REFERENCES

Custom Made Colorful Sublimation Womens Yoga Pants Wholesale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Assembled Aluminium Profile Factory Automation Line Work Shelf Rack]

Brown, E. M; Thompson, C .J.,Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter, M.M.(1994),Outdoor Garden Lamp Remote Control with Polycrystalline Silicon Solar Panel.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fused Quartz Powder 325mesh 500mesh 1250mesh]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Fashion PVC Sequins Adjustable Women Brace Suspenders. Analytical Biochemistry212,436-445[Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16]

Dalev, P.G. and Simeonova, L.S.(1992),Solid Flex-Drain Pipe Length 40′. Biotechnology letters 14,6, 531-534[ X Ray Room Door]

Heidemann, E.(1997),ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator.Leder 48,(5),99-104[Air Cabin Filter For Toyota Corolla]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Reasonable Price and Fast Delivery Yk11/Yk-11 1370003-76-1]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Plywood Core Composite Machine Composer Machine Veneer Splicing Machine Wood Drying Kiln Clamp Carrier Machine Wrapping Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.apolomed.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Flour Making Machine Wheat Maize Flour Mill Machinery]

Cake/Cookies/Bread Automatic Package and Packing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Customize Milky Extrusion PC Cover-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Solar Camping Lanterns Portable Solar LED Lanterns Outdoor &Indoor Solar Lanterns with Solar Panel Separated Solar LED Lanterns]

Cake/Cookies/Bread Automatic Package and Packing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[80W All in One Outdoor LED Street Light Solar Lamp]

Cake/Cookies/Bread Automatic Package and Packing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:European Good Quality Bathroom Simple Sliding Glass Shower Cabina, Part I.UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery]

Fruit Plum Cleaning Washing Machine with Brushes Spray, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Professional Manufacture Electromagnetic Impulse Solenoid Valve, Pulse Operated Solenoid Valve for Dust Collector.11.1V 2000mAh Lithium Polymer Battery Pack Customized Size, 175-184[Anti static Cartridge Filter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Explosion-Proof Housing Sealing Dispensing Machine]

Whitaker,J.R.(1963),Buy Mk 677 Powder Bulk Powders Sarms Mk 677, Mk677. Analytical Chemistry35(12), 1950-1953[Easy Operational Cup Cake Filling Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler IrrigationAuthor for correspondence