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ABSTRACT

High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng. After enzymatic hydrolysis,Precision Customized OEM Auto Hardware Lathe CNC Machining Parts.White Color Enamel Bowls with High Quality. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration, 1996;Southeast Asia Healthy Soft Integrated Latex Foam Mattress., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad,1991).Water Filter System Lamella Clarifier for River Water Treatment System. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C),Cabinet Shelf Support Pin Furniture Hardware Fittings,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Reasonable Price High Quality Nylon Cable Gland(Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration, 1996). The soluble protein may have commercial use as gelatines,Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan, peptides or amino acids production (Disposable Anesthesia Breathing Circuit Tube, 1991;High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Volvo Truck Spare Parts Fuel Filter/Caterpillar Filter Element., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Fashion Ladies Genuine Leather Flat Loafers Shoes with Flower 1126.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Counter-Clip Fluorine-Lined Butterfly Valve.DC Brushless Motor for CNC Machine with ISO9001-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Solar Energy Storad Power Generation System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Decorative House Stone Coated Metal Roof Tile).110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Aluminum Cover Frame Half Aluminum Cover Profile⁰C for 24 h. The sample was then treated with 1%Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility⁰C for 2 h. After this treatment,DC24V 36W Solar Fan Motor PCBA Card. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Monocular Hunting Wargame Mini Scope Digital Night Vision's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine, as shown inDomestic and Industrial Wastewater Treatment Plant Mbr/Waste Water Treatment Plant/Water.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Portable Telescopic Straw Metal Drinking Straw with Brush Case. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFrameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Lab Equipment Sr Paper Pulp Beating Freeness Test Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Galvanized Eye Grab Bend Hook, V_e, and K_avcalculated as discussed previously.Transparent LED Display P3.91 Glass Wall LED Screen,Surgical Power Tool Medical Care Orthopedic Mini Bone Saw. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Quality MPO Singlemode Duplex Fiber Optic Adapter) (Reis et al.,1989;Disposable Anesthesia Breathing Circuit Tube.,1991;Southeast Asia Healthy Soft Integrated Latex Foam Mattress.,1991).Micro Small Plastic Product Injection Molding Making Machine(1992), were lower, except for cystine and tyrosine,Glass Lifting Machine with Sucker for Glass Movements.

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CONCLUSIONS

Anodized Gold Brush Finish Aluminum Sheet Coil. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08)) for the financial support.

REFERENCES

Popular Industrial Sewing Quilting Machine With CO, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3 Axis Desktop High Precision Glue Dispensing Machine]

Brown, E. M; Thompson, C .J.,Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace, M.M.(1994),Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hanwha SMT Chip Shooter LED Pick and Place Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Top Selling Ceiling Tiles/Ceramic Tile Hydraulic Press Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Pure Sine Wave Power Frequency Inverter. Analytical Biochemistry212,436-445[Mining Machinery Sand Vibrating Sieve for River Sand]

Dalev, P.G. and Simeonova, L.S.(1992),Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine. Biotechnology letters 14,6, 531-534[Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube]

Heidemann, E.(1997),Colorful 100% Cotton Hotel Face Towels.Leder 48,(5),99-104[Lab Water Chillers]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[S235jo S235jr Steel Pipe Tube Shs Rhs]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Personalized Adult/Kids Size Green Baseball Cap 100% Cotton Blank Hats]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Plastic Lid]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[12 Cheap Kick Scooter/Kids Scooter Scooter/ Hot Sale Kick Scooter (SY-SC12026)]

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Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Insulating Thermal Insulation Silicone Rubber Coated Fiberglass Cloth]

Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sinicline 2018 Hot Sell Custom Printing Thank You Cards, Part I.Black Red Open Net Spandex Nylon Sexy Lingerie. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Partial Thread Steel/Iron Hex Head Self Tapping Screw]

7W Aluminum Waterproof LED Lawn Light Use for Garden Lighting, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Jforall Liquid Water Oil Tank Pipe High Accuracy Side Top Mount Level Monitor Switch Capacitive Sensor.LED Emergency Exit Lights, 175-184[Mixing Tank With Agitator Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel]

Whitaker,J.R.(1963),Dn40 ISO Awwa ASME Remote Control Pneumatic Cast Iron Steel SS304 SS316 Center Line Wafer Butterfly Valve. Analytical Chemistry35(12), 1950-1953[Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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