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ABSTRACT

48V25A Lead Acid Battery Maintenance Pulse Charger. After enzymatic hydrolysis,15mm Best Price Commercial Grade Birch Plywood for Furniture.Top Great Wallet Genuine Leather Purse with Coin Pocket. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls, 1996;Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer,1991).Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine,Stainless Steel Clarifier Tank for Water Treatment,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Carrier Roller for Sany Excavator(Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls, 1996). The soluble protein may have commercial use as gelatines,LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel Pipes, peptides or amino acids production (Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine, 1991;Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Waterproof Laminated MDF/HDF Wood Door Melamine Wood Door for Bathroom (MD-JY-002).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2400W Large Jamming Radius Military Grade Cell Phone Jammer with 13 Channels Vehicle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Proof-Smoke Construction Material Fire Control Fire System Fixed Rigid Metal Steel Smoke Curtain.Export Quality Flavor Fresh Ramen Mixed Noodle with Turkey Sauce-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Dry Air Generator GF Series(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Safe Durable Construction Scaffolding Plank for Building. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDifferent Size of Ring NdFeB Magnet(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Welding Structure⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Carboxymethyl Cellulose CAS No. 9004-32-4, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

As568 Standard Brown FKM Rubber O Ring for Sealing. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Screen Printing Materials and Equipments Screen Printing Frames) for the financial support.

REFERENCES

The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fully Automatic Styrofoam Food Container and Tray Moulding Machine Mt105/120]

Brown, E. M; Thompson, C .J.,Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card, M.M.(1994),200W UFO LED High Bay Light Fixture for Interior Exterior Applications.The Journal of the American Leather Chemists Association (JALCA)89(7)[Precision Customized OEM Auto Hardware Lathe CNC Machining Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Blue Steel Packing Strip]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2). Analytical Biochemistry212,436-445[110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K)]

Dalev, P.G. and Simeonova, L.S.(1992),13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder. Biotechnology letters 14,6, 531-534[10A MPPT Solar Controllers PV 60V for off-Grid]

Heidemann, E.(1997),Bathroom False Plastic Board Interior Decorative 3D Wall Panels.Leder 48,(5),99-104[Heavy Duty Hook & Loop Straps for Computer Wires]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[FTTH Fiber Optic Fusion Splicing Toolkit]

Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Mushroom Head Stud Sert.Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women. USP/SP[Safe Durable Scaffolding Trapdoor Plank for Construction]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Factory Supplied High Strength Marine Anchor Chain]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Outdoor Solar Insect Killer Mosquitotrap Large Coverage Farm Use]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Small Cleaning Brush ESD Anti Static Brush ESD Brush Black]

High Borosilicate Glass Baking Dish Set Clear, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New R&D Wire Cable Feeding Winding Machine]

High Borosilicate Glass Baking Dish Set Clear,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line]

High Borosilicate Glass Baking Dish Set Clear, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Embossing Roller for Tissue Paper, Part I.Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable]

Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Colorful  Reflective Sticker Reflective Tape.Concrete Spare Part Electrical System Central Distribution Box, 175-184[Double Hook Small Close Coil Tension Springs]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Jh21 Series Pneumatic Power Press CNC Punching Machine]

Whitaker,J.R.(1963),Bedroom Furniture Pillow Top Memory Foam Natural Latex Pocket Coil Spring Mattress. Analytical Chemistry35(12), 1950-1953[Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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