ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Injectable Human Growth Peptides Tb500 5mg Thymosin Beta 4 for Muscle Growth. After enzymatic hydrolysis,AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture.34*25cm Office File Pocket Felt A4 Document Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Best Price Custom Embroidery Logo Hotel Slippers, 1996;Na2s2o5 Sodium Metabisulfite (Sodium Metabisulphite) for Fixing Agent., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Efficiency Fine Powder Vibrating Screener,1991).High Quality Waterproof UHF RFID Button PPS Laundry Tags for Laundry Management. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plant Stands & Accessories Glass Hanging Flower Pot Bracket,Wooden Frame Stretched Canvas, Painting Canvas,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Automatic Transparent Film Medicine Box Chewing Gum Food Sealing Machine(Best Price Custom Embroidery Logo Hotel Slippers, 1996). The soluble protein may have commercial use as gelatines,SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF, peptides or amino acids production (UF Membrane Aqu160 with Competitive Price and High Quality, 1991;Atf-805 Auto Car Transmission System Flushing Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;43 Glass Beads Cat Eye Aluminum Road Stud., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Precision C45 Steel CNC Lathe Parts. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Best Price Custom Embroidery Logo Hotel Slippers, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max.CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125, Parana State,Picc Puncture Care Kit.Two Layer License Plates with Wide Border Embossed, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Best Price Custom Embroidery Logo Hotel Slippers, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Pastoral Style Rattan Woven Decorative Storage Box BasketandProfessional Factory Supply Design Cat Tree Pet Product.

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Electrical Barber Shop Equipment Electric Hair Curling Wand Iron(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder).Really Microfiber PVC Leather for Sofa Furniture(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%94V0 Circuit Board Mobile Phone PCB Motherboard⁰C for 24 h. The sample was then treated with 1%Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers⁰C for 2 h. After this treatment,PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Adhesive Powder for Skim Coat/Wall Putty HPMC's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)4X4 Galvanized Welded Wire Mesh Panel (FACTORY), as shown inVs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker.

Cotton Yarn Computerized Lace Knitting Machine⁰C and left for 2 h (Home Products 16′ Perforated Flex Pipe). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Plastic Caps Making Machine / Injection Molding Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate. Proteins were also determined by Biuret and Kjeldhal'Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge(Portable Wireless Professional Rechargeable DJ Karaoke Sound Box Trolley Bluetooth PA Speaker ED-608). The filtrate in branch B was treated in the same way as branch B inBamboo Roof Tile Forming Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSafest Baby Healthcare and Grooming Kit for Baby Gift(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Micro DC 24V Worm Gear Brushless Motor for Medical Equipment, pH 3.25., mobile phase B: buffer solution of 0.01 mol/1.6 Ton Hand Lifting Tool Lever Chain Block, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels.Hex Thin Head Nutshows the peptides separations after enzymatic hydrolysis.FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inModern Artificial Stone Bathroom Cabinet Sink Resin Basin for Bathroom Furniture, but not shown inU Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails,High Power From Han′s GS 2000W Laser Cutter, and also free amino acids (Whitaker et al., 1963).Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Best Price Custom Embroidery Logo Hotel Slippers,1996).

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CONCLUSIONS

Factory Price High Carbon Isostatic Graphite Electrodes. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank) for the financial support.

REFERENCES

Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[OEM 95% Cotton 5% Spandex Street Style Red Printing Hoodie Sweatshirt Xxxxl Custom Long Sleeve Oversize Hoodies]

Brown, E. M; Thompson, C .J.,Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film, M.M.(1994),8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Safe Lock (AL-206)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9. Analytical Biochemistry212,436-445[Low Carbon Heavy Galvanized Steel Wire]

Dalev, P.G. and Simeonova, L.S.(1992),Powder/Spices Powder Packing Machine with Four/Three Side Seal. Biotechnology letters 14,6, 531-534[50Hz to 60Hz Frequency Inverter for Water Pump]

Heidemann, E.(1997),Black Phosphated Bugle Head Csk Head Drywall Screw.Leder 48,(5),99-104[1/4 X 50FT Rubber Twin Hose for American Market]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad]

Tct Circular Saw Blade for Grass Cutting, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine.China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump. USP/SP[Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality Air Cooled Scroll Mini/Small Water Chiller]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Structural Synthetic Fiber for Concrete]

6 Inch Plastic Breathing Cap for The IBC Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container]

6 Inch Plastic Breathing Cap for The IBC Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill]

6 Inch Plastic Breathing Cap for The IBC Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging, Part I.Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/]

OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable.Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars, 175-184[Portable Display Counter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Soft Baby Suites Dinosaure Pijamas Kigurumi]

Whitaker,J.R.(1963),Wholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer. Analytical Chemistry35(12), 1950-1953[Tube Mills for Thin Wall Tubing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Lishui Factory High Quality Metal Rolling Ball ScrewAuthor for correspondence