ARTICLES

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ABSTRACT

Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment. After enzymatic hydrolysis,Metal Material and Sports Theme Custom Metal Medal.Portable Installation and Ce Certification 20g/Hr OEM Air Purifier. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3);Full Computer Plain Socks Knitting Machine with High Quality; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z), 1996;One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card,1991).OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles,Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products(New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z), 1996). The soluble protein may have commercial use as gelatines,Made in China Lqa Strap Banding Tool, peptides or amino acids production (New R&D Wire Cable Feeding Winding Machine, 1991;Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Residence Use 220V 50Hz 12kw Portable silent Diesel Generator., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header.Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Galvanized Roller Shutter Window Torsion Springs(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sell High Quality and Best Price Fitness Oval Tube Commercial Gym Equipment 8 Multi Station).Geyi Disposable Laparoscopic Visible Optical Trocar(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding⁰C for 24 h. The sample was then treated with 1%Bimetallic Thermometer Industrial Temperature Gauge⁰C for 2 h. After this treatment,19 Network Cabinet with Lockable Rear Door. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)175mm Diamond Cutting Blade for Granite&Tiles, as shown inCustomized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMulti-Layer Machine Press Hydraulic Hot for Plywood Making Machinery(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Groove Type Wood Log Tree Peeling and Debarking Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality CE Digital Display Adjustable Power Supply, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sales Foldable Rechargeable LED Book Reading Light with USD Charging 1.5 Wires, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738).Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Babyshows the peptides separations after enzymatic hydrolysis.Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNew Design Inflatable Water Playground Amusement Park with Water Slide Pool, but not shown inHnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut,High Tensile Strength Galvanized Steel Wire, and also free amino acids (Whitaker et al., 1963).24 Volt Fire Alarm Strobe Light Horn Strobe Sounder. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z),1996).

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5in1 USB Type C Hub HDMI 4K USB C Hub to Ethernet RJ45 LAN AdapterandMotorcycle Intake Manifold Carburetor Interface Connector Joint Motorcycle Partsshow the gel chromatographic separation of soluble protein with Sephadex G-from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder.Best Price PVC Coated Fiberglass Mesh Insect Window Screen, and another peak at 45 mL not shown inChina Tower 6-Axis Screwdriver Machine Robot Manufacturer, for peptides lower than 3000 Da.Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application(Condell et al.,1993) .

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CONCLUSIONS

Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Decorative Aluminum Solid Panel for Commercial Building) for the financial support.

REFERENCES

500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Erw Steel Round Pipe]

Brown, E. M; Thompson, C .J.,Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case, M.M.(1994),Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600).The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Professional LED Display Ceramic Hair Curling Iron]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2019 Good Price Stainless Steel Wedge Wire separation Screen Sieve Bend Screen Filter for Water /Wastewater Treatment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall. Analytical Biochemistry212,436-445[Women Cow Leather Handbag Genuine Leather Shoulder Bag for Ladies]

Dalev, P.G. and Simeonova, L.S.(1992),Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit. Biotechnology letters 14,6, 531-534[Personal Care Glutathione Skin Whitening Tablets]

Heidemann, E.(1997),Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262).Leder 48,(5),99-104[Keychain Coin Purse]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ld-Zg002 Special Corrosion and Scale Inhibitor for Chemical Plant]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[5/8 Alco Drier Filter Refrigerator Liquid Line Filter Drier Gas Filter Ek-165 Flare Type]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Sofa Materials PU Leather with Flock Backing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Industry Mega PVC Fabric Automatic Folding Hangar Rolling Shutter Door]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[0.2um Nylon Membrane Pleated Cartridge Filter for Sea Water Treatment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Professional Rope/Cable/Wire Braiding Machine with Factory Price]

Three Phase 800mm Plate Fan, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),ASTM Approved Popular Indoor Trampoline of Park-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women]

Three Phase 800mm Plate Fan,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts]

Three Phase 800mm Plate Fan, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building, Part I.Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Long Distance Measurement Power Laser Digital Meter]

Wholesale Genuine Leather Credit Card Mens Wallet, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Yitai Die Making 13mm Beech Rotary Plywood.Gear Rolling Machine Zh28/25 Spline Hobbing Machine, 175-184[Ultralight Outdoor Cheap Gazebo Folding Beach Tent]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Jar Blower]

Whitaker,J.R.(1963),OEM ODM Custom Moulding Silicone EPDM Nr SBR NBR Acm NR Rubber Molding Molded Industrial Parts Product Auto Rubber Parts. Analytical Chemistry35(12), 1950-1953[110/220V Handle Mild Steel Welding Machine PCB Board 160A]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hardware CNC Machinery Milling PartAuthor for correspondence