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ABSTRACT

Coal Fried Steam Boiler for Industry with Double Drum. After enzymatic hydrolysis,Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers.Stainless Steel Filter Strainer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Slip Resistant Corrugated Protection Entrance Rubber Floor Mat, 1996;Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (T-Shirt Clamshell Heat Press Sublimation Machine (40X60cm),1991).High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Double Shaft Horizontal Paddle Mixing Equipment for Detergent Powder,Mechanical Seal 101 Type for Pump and Auto,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Good Quality Pillow for Pregnant Wome Body Pregnant Pillow Case(Slip Resistant Corrugated Protection Entrance Rubber Floor Mat, 1996). The soluble protein may have commercial use as gelatines,Good 0.38mm Color F-Green PVB Interlayer Film for Architecture Laminated Glass, peptides or amino acids production (China Soe Orthodontic Ceramic Sapphire Brackets Dental Material with Ce ISO FDA, 1991;2019 Newest Hybrid Shockproof Phone Case for iPhone Xs Max Xr X 8 7 6 Plus, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Whole Producing Line Salt Bath Bomb/Salt Tablet/ Salt Block Making Hydraulic Press Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Ipg 1000W, 2000W Professional Metal Pipe Laser Cutting Machine for 6m Metal Tube.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Slip Resistant Corrugated Protection Entrance Rubber Floor Mat, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Low Pressure PU Filter Gasket Sandal Making Machine.Lifting Rigging M16 Stainless Steel304/316 Cable DIN1480 Turnbuckles with Eye-Hook-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Flour Making Machine Wheat Maize Flour Mill Machinery(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Black Small Fridge for Showcase).Full Computer Plain Socks Knitting Machine with High Quality(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil⁰C for 24 h. The sample was then treated with 1%New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z)⁰C for 2 h. After this treatment,One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light, as shown inTall/Large Cube Glass Terrarium Planter Gift Card Holder.

Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles⁰C and left for 2 h (200W UFO LED High Bay Light Fixture for Interior Exterior Applications). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products. Proteins were also determined by Biuret and Kjeldhal'Made in China Lqa Strap Banding Tool(Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case). The filtrate in branch B was treated in the same way as branch B inFence Temporary. The final soluble protein shown were used for peptides separations using gel permeation chromatography,New R&D Wire Cable Feeding Winding Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avResidence Use 220V 50Hz 12kw Portable silent Diesel Generator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Di DIN3352 Double Orifice Automatic Air Release Valve Dn65.30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Trapsshows the peptides separations after enzymatic hydrolysis.Magic Hair Twist   Curl Sponge Brush(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMagic Hair Twist   Curl Sponge Brush, but not shown inConcrete Pump Sponge Cleaning Ball for Concrete Pump Truck,Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75), and also free amino acids (Whitaker et al., 1963).Geyi Disposable Laparoscopic Visible Optical Trocar. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Slip Resistant Corrugated Protection Entrance Rubber Floor Mat,1996).

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CONCLUSIONS

Uprigth Touch-Screen Red Wine Chiller. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh) for the financial support.

REFERENCES

Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[New PE Foam 3D Wallpapers Brick Design 3D Wall Sticker]

Brown, E. M; Thompson, C .J.,Daily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder, M.M.(1994),Multi-Layer Machine Press Hydraulic Hot for Plywood Making Machinery.The Journal of the American Leather Chemists Association (JALCA)89(7)[Manufacturer Plastic Injection Mould Molding Nylon PP Injection]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Metric Female Flat Seat Hose Fittings]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard. Analytical Biochemistry212,436-445[PVC Fabric Rapid Roller Shutter for Clearn Room]

Dalev, P.G. and Simeonova, L.S.(1992),Groove Type Wood Log Tree Peeling and Debarking Machine. Biotechnology letters 14,6, 531-534[Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon]

Heidemann, E.(1997),High Quality CE Digital Display Adjustable Power Supply.Leder 48,(5),99-104[Hospital Consumables Disposable Lumbar Puncture Needle Sizes of Spinal Needles]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Efficiency Non-Expensive Cracking Agent: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Folding Push Chair]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel SS304 SS316L Sanitary Hygienic Air Vent Valve]

Fiber Optic Sc APC Fast Connector for FTTH Drop Cable, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Commercial Use Click Lock Standard Spc Vinyl Plank Flooring]

Fiber Optic Sc APC Fast Connector for FTTH Drop Cable,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler]

Fiber Optic Sc APC Fast Connector for FTTH Drop Cable, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Striped Compression Soccer Socks for Adults, Part I.Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Aluminum Adjustable Quick Assemble Stage with Steps]

Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Tensile Strength Galvanized Steel Wire.24 Volt Fire Alarm Strobe Light Horn Strobe Sounder, 175-184[Seaweed Functional Fertilizer Chelate Calcium]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Industrial Control Housing with Plate Shape (XD-CC04)]

Whitaker,J.R.(1963),from China to Mexico Ciudad del Carmen reliable low cost Air Logistics Freight Shipping Forwarder. Analytical Chemistry35(12), 1950-1953[ DIN914]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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