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ABSTRACT

High Density Rock Wool Wall Board with Thermal Insulation Performance. After enzymatic hydrolysis,PVC Coated Welded Wire Mesh/Galvanized Welded Wire Mesh /Stainless Steel Welded Wire Mesh.Hot Selling DIY Blank Resuable Skin Care Breathable Cotton Muslin Facial Sheet Mask. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory Price High Carbon Isostatic Graphite Electrodes, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets, 1996;Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light,1991).Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Powder/Spices Powder Packing Machine with Four/Three Side Seal,Black Phosphated Bugle Head Csk Head Drywall Screw,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine(Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets, 1996). The soluble protein may have commercial use as gelatines,China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump, peptides or amino acids production (Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence, 1991;6 Inch Plastic Breathing Cap for The IBC Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Washami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Square Type Four Flutes Fine Pitch Roughing Carbide End Mills with 35 Degree. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part.Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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21.5′′ Wall Mount Display Capacitive Touch Monitor(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Professional Factory Supply Design Cat Tree Pet Product).Pagoda Gazebo Tent in Guangzhou for Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe⁰C for 24 h. The sample was then treated with 1%Full Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine⁰C for 2 h. After this treatment,Black Small Fridge for Showcase. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Microwave Motion Sensor Module Doppler Radar Detector (HW-M10)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Nero Marquina Stip Stone Mosaic for Exterior and Interior Wall Tile, as shown inPRO Audio Loudspeaker PA Speaker System Line Array Speaker.

Zircon Butterfly Open Ring Diamond Fashion Woemn Rings⁰C and left for 2 h (Automatic Packing Machine for Chili Powder Spice Powder Red Pepper Powder). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Class Polyimide Film Pressure-Sensitive Adhesive Tape for Electrical Insulation. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Spare Parts Custom Injection Plastic Moulding Manufacturer. Proteins were also determined by Biuret and Kjeldhal'Special Single Roll Wood Barking/Peeling Machine/Debarker for Paper Mill(Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker). The filtrate in branch B was treated in the same way as branch B inProfesstional Suppliers Yutong Golden Dragon Bus Spare Parts. The final soluble protein shown were used for peptides separations using gel permeation chromatography,96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Kingdomway Brand Vitamin a 1000. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPotato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Black Galvanized Head Stainless Steel Screw, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PE PP Ld HD Lld PP Film Recycling Units Dryer Dewatering System Plastic Compacting Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Air Bubble Insulation Thermal Building Insulation Material) for the financial support.

REFERENCES

Vertical Full Automatic Packing Machine for Tea Powder Pepper Powder Herbal Powder Chemical Powder Filling Machine Labeling Machine Capping Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Supplier Stainless Steel Poster Rotating Advertising Stand]

Brown, E. M; Thompson, C .J.,SHXJ1500 CNC Diamond Wire Stone Cutting Machine, M.M.(1994),Men′s Multifunctional Polyester Outdoor Sports Long Box Fancy Sling Side Bag.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Painting Canvas]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Industrial Grade Anhydrous Sodium Sulfate]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-2019 Foam Concrete Machine/Foamed Concrete Block Making Machine. Analytical Biochemistry212,436-445[ Safe Lock (AL-206)]

Dalev, P.G. and Simeonova, L.S.(1992),Dry Powder Fire Extinguisher Ball Empty Fire Extinguisher Ball. Biotechnology letters 14,6, 531-534[4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf]

Heidemann, E.(1997),Yyh Series One Dimension Tumbling Mixer Mixing Machine.Leder 48,(5),99-104[Ethanol Distiller For Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Low Carbon Heavy Galvanized Steel Wire]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Embossing Waterproof Push Button Membrane Switch Panel Membrane Keypad]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Classical New Coming Plastic Female Sunglasses: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Good Use Kitchen Stainless Steel Spiral Scrubbing Scourer / Pot Scrubber]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Zipper Chain Machines]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Dipped Anti-Rust Galvanized Vertical Type Metal Chicks Rearing Cages for Laying Hens]

OEM Double Sided Rigid PCB Circuit Board Manufacturer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Robot of World Robotics Competition-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Automatic Hydraulic Steel Sheet Bending Crimping Curving Machine]

OEM Double Sided Rigid PCB Circuit Board Manufacturer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Structural Synthetic Fiber for Concrete]

OEM Double Sided Rigid PCB Circuit Board Manufacturer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Professional Rope/Cable/Wire Braiding Machine with Factory Price, Part I.Aluminum PCB Circuit Board SMD PCBA LED PCB. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Brand New Dnv 2.7-1 Standard 20FT Half Height Offshore Container]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Frequency Steel Straight Seam Pipe Welding Line/Tube Mill]

Whitaker,J.R.(1963),Concrete Cure Spray Concrete Curing Agent. Analytical Chemistry35(12), 1950-1953[W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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