ARTICLES

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ABSTRACT

Ferrule Woven Rope Wire Mesh /SS316 Decorative Rope Mesh. After enzymatic hydrolysis,Integrated Wastewater Treatment Equipment for Poultry and Slaughtering Sewage Treatment.Metal Material and Sports Theme Custom Metal Medal. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Portable Installation and Ce Certification 20g/Hr OEM Air Purifier;Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Full Computer Plain Socks Knitting Machine with High Quality, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil, 1996;New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (One Step Myo/Ck-MB/Ctnl Combo Rapid Test Cassette,1991).Hot Sell RFID 13.56mkz MIFARE 4K DESFire EV1 CPU Card. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,OEM Factory Supplier Global Fashion No Light Color Gel Nail Polish Without UV Light,Shingles Wholesale Building Material Roofing Materials Spanish Sheet Roofing Tiles,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Natural Latex Pocket Coil Spring Mattress for 5 Star Hotel(Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil, 1996). The soluble protein may have commercial use as gelatines,Flex Rigid Printed Circuit Board with UL Flex PCB for Electronic Products, peptides or amino acids production (Made in China Lqa Strap Banding Tool, 1991;New R&D Wire Cable Feeding Winding Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Manual Glass Kitchen Ajustable Pepper with Low Price., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Residence Use 220V 50Hz 12kw Portable silent Diesel Generator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Garden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰530ml Glassware/Glass Jar/Wine Bottle/Liquor Bottle/Spirits Bottle/Glass Vodka Bottle.PCB Connector Pin Connector 1.0 Pitch Dual Row Pin Header-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Sdlg LG936L Wheel Loader Spare Parts 4041000687 Hose Assembly(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Magic Hair Twist   Curl Sponge Brush(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Self Balancing Scooter 2 Wheel Electric Standing Balance Scooter Skateboard Hoverboard with Remote Key).Clearing Clogged Effectively Pipe Drain Cleaning Machine (A75)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Geyi Disposable Laparoscopic Visible Optical Trocar⁰C for 24 h. The sample was then treated with 1%Customized Plastic Items Access Flap Cover Cap Plastic Injection Moulding⁰C for 2 h. After this treatment,Bimetallic Thermometer Industrial Temperature Gauge. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)19 Network Cabinet with Lockable Rear Door's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Automatic Blow Molding Pet Bottle Making Semi Auto Blower Perform Making Bottle Machinery, as shown inAluminum Foil PE Bottle Cap Induction Seal Liner.

175mm Diamond Cutting Blade for Granite&Tiles⁰C and left for 2 h (Racing Preformance PTFE Teflon Alloy Fuel Adapter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Raytools/Wsx/Precitec Fiber Laser Cutting Head, Laser Cutting Nozzle, Lasr Cuting Protective Lens, Laser Cutting Ceramic, Laser Cutting Consumables. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,PU-Flex Duct Hose for Transporting Dust, Granules and Shavings. Proteins were also determined by Biuret and Kjeldhal'Euro Schuko 3 Pin Plug to IEC 320 C13 Female Connector 10A 220V AC Power Plug(Air Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length). The filtrate in branch B was treated in the same way as branch B inDigital And Analog Dissolved Oxygen Analyzer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Uprigth Touch-Screen Red Wine Chiller.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDaily Use Teeth Whitening Charcoal Powder Oral Hygiene Cleaning Activated Charcoal Powder(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Yashi 42 Inch LED Multi Touch Screen Interactive Whiteboard⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Groove Type Wood Log Tree Peeling and Debarking Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality CE Digital Display Adjustable Power Supply, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Custom Striped Compression Soccer Socks for Adults.2mm Mirror Glass/Coated Glass for LEDshows the peptides separations after enzymatic hydrolysis.Stainless Steel Kitchen Sink, Bar Sink, Wash Hand Sink (4738)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFood Machine Cube Ice Machine for Commercial Use, but not shown inHigh Quality Powder and Wax Brush with Wooden Handle,Mining Machine Dewater Screen Separator Coal Sand Sieve Shaker Dewatering Screen, and also free amino acids (Whitaker et al., 1963).High Tensile Strength Galvanized Steel Wire. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil,1996).

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Abrasive Resistant Heavy Duty PVC Helix Suction Hose for Mining Application, V_e, and K_avcalculated as discussed previously.Best Selling Used Plastic Single Shaft Shredder for Recycling with Weg Motor,Factory OEM CNC Machined Gear Motor Shaft. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High Precision Piston Ring Good Shape Ability High Quality Surface) (Reis et al.,1989;Made in China Lqa Strap Banding Tool.,1991;New Style LiFePO4 Battery Powered Electric City E-Bike, Bicycle Moped with Pedals with 36V 250W Motor (JB-TDE23Z).,1991).High Quality and Lowest Price of Crr920 Common Rail Injector Repair Kits to Meet Stage(1992), were lower, except for cystine and tyrosine,Pink French Loop Terry Knit Fabric for Jacket.

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CONCLUSIONS

Custom Plastic Parts Injection Molding Lamp Shell Cover for LED Bulb. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Carbon Graphite Crucible) for the financial support.

REFERENCES

Die Casting Aluminum Shell Aluminum Body for Electrical Motor Parts / Air Compresser Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Miniature Right Angle Gears]

Brown, E. M; Thompson, C .J.,500W Outdoor Lighting LED Wall Lamp Solar Light LED Flood Street Light with Controller IP65 Wall Light with Solar Panel, M.M.(1994),Music Equipment Flight Case/Aluminum Flight Case Hardware/Guitar Effect Pedals Flight Case.The Journal of the American Leather Chemists Association (JALCA)89(7)[Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM Refrigeration Digital Thermometer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Electric Fabric and Non Woven Fabric Roll Slitting Machine (FQ-1600). Analytical Biochemistry212,436-445[Residence Use 220V 50Hz 12kw Portable silent Diesel Generator]

Dalev, P.G. and Simeonova, L.S.(1992),Builtin Video Wall Controller 3X3 LCD Digital Signage Large Video Wall. Biotechnology letters 14,6, 531-534[High Strength New 0.18mm Molybdenum Wire]

Heidemann, E.(1997),Gsx750f Gsf1200 Bandit 01-06 Carb Repair Kit.Leder 48,(5),99-104[Fuel Injector 25342385A for Chevrolet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Lab Equipment Sr Paper Pulp Beating Freeness Test Machine]

Flour Making Machine Wheat Maize Flour Mill Machinery, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Charateristic Natural Anodized Aluminium Al6061-T6 CNC Machined Housing for Embroidered Beauty Pen (S-262).Cute Pink Cashmere Soft Plush Lining Warm Bootie. USP/SP[Disposable Precision Infusion Set with Filter and Needle for Single Use]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ycom USB Charger and Data Cable with Aluminum for Type C]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-3kw 24V 310mm Vertical Agv Drive Wheel Assembly: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Anodized Gold Brush Finish Aluminum Sheet Coil]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Silver Aluminium Hanging Display Snap/Poster/Photo Frame Stand]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed]

Sofa Materials PU Leather with Flock Backing, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Three Phase 800mm Plate Fan-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly Valve]

Sofa Materials PU Leather with Flock Backing,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table]

Sofa Materials PU Leather with Flock Backing, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:ASTM Approved Popular Indoor Trampoline of Park, Part I.Cheap Bent 6mm 8mm 10mm 12mm Clear Curved Tempered Glass for Construction Building. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net]

Adjustable Office Commercial Suspended Pendant LED Aluminum Channel Profile for Ceiling Light, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Genuine Leather Credit Card Mens Wallet.Yitai Die Making 13mm Beech Rotary Plywood, 175-184[Soft Water Equipment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Two Layer License Plates with Wide Border Embossed]

Whitaker,J.R.(1963),Gear Rolling Machine Zh28/25 Spline Hobbing Machine. Analytical Chemistry35(12), 1950-1953[Horizental Stainless Steel Food Grade Milk Tank for Milking]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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