ARTICLES

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ABSTRACT

4X4 Galvanized Welded Wire Mesh Panel (FACTORY). After enzymatic hydrolysis,Cotton Yarn Computerized Lace Knitting Machine.Plastic Caps Making Machine / Injection Molding Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate;Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fly, Flry-a, Flry-B Automotive Cable DIN72551, Wire Cable, Wire Harness, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light, 1996;Safest Baby Healthcare and Grooming Kit for Baby Gift., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fashion Birthday Gift 18K Gold Plated Jewellery 925 Sterling Silver Custom Name Plate Bracelet for Women Men Baby,1991).Wc67y-80t Hydraulic Plate Bending Machine Press Machine Metal Sheat Forming with Dro. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Micro DC 24V Worm Gear Brushless Motor for Medical Equipment,1.6 Ton Hand Lifting Tool Lever Chain Block,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,CNC Machining Service High Precision CNC Mechanical Parts(LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light, 1996). The soluble protein may have commercial use as gelatines,Home Decoration Perfume Natural Reed Aroma Diffuser with Rattan Sticks, peptides or amino acids production (Vs1-12/630A Indoor Vs1 Vacuum Circuit Breaker Handcart Type High Voltage Vacuum Circuit Breaker, 1991;Printed 100% Polyester Tcheap Wholesale Blankets, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Food Grade Material 304/316 Double Diaphragm Pumps., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Manufacturer Wholesale Welded Wire Mesh Large Dog Cage/Dog Run Kennels/Dog Run Fence Panels.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-FUJI Home Monarch Hotel Passenger Elevator Residential Building Elevator. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Power From Han′s GS 2000W Laser Cutter.Glazing Fixed-Point Facade Mullion Curtain Wall Outdoor Indoor Glass Panel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Widely Application Latest Designed Coffee Capsule Filling Packing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China PAC Water Flocculating Agent for Water Treatment(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Animal Agricultural Damping Horse Stall Rubber Cow Mat).Factory Price High Carbon Isostatic Graphite Electrodes(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Good Quality 3.8 6 8 Inch Resistant Rubber Tire Dog Chew Toy with Handle Rope for Pets⁰C for 24 h. The sample was then treated with 1%Rainbow Metallized Polyester / Holographic Embossing Metallized Pet Film⁰C for 2 h. After this treatment,8W IP65 CCT Changeable Dimmable Fire Rated LED Downlight Bezel Interchangeable Fireproof Down Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Price 0.5ml 1.0ml Ceramic Wickless Vape Cartridge Liberty V9's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Powder/Spices Powder Packing Machine with Four/Three Side Seal, as shown in50 Grams Ozone Producing Device for Makeup Toner Producing Plant.

Black Phosphated Bugle Head Csk Head Drywall Screw⁰C and left for 2 h (3D CNC Router for Wood Cutting Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Liquid Detergent, Shampoo Mascara Titling Mixing and Emulsifying Making Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump. Proteins were also determined by Biuret and Kjeldhal'Cbt-65 Type Hot Dipped Galvanized Concertina Razor Barbed Wire for Fence(High Quality 4X2 and 6X4 China Heavy Duty Tractor Truck HOWO Tractor Truck). The filtrate in branch B was treated in the same way as branch B in3d Printer With Led. The final soluble protein shown were used for peptides separations using gel permeation chromatography,6 Inch Plastic Breathing Cap for The IBC Valve.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Efficiency High Precision PT52y-X Turning Milling CNC Machine for High Quality and Reliability. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWashami Wholesale 2 in 1 Custom Fashion Lip Gloss Packaging(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.OEM Carbon Steel/Stainless Steel Silca Sol Lost Wax/Investment/Precision Cast/Casting Part⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Central Loose Tube No Armored 2 - 24 Core Fiber Optic Cable, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for CocktailandWholesale Girls Dresses Printed Rayon Fabric From Textile Manufacturer. Enzymatic (Freshwater Pearl Butterfly Flower Bride Hairpin)Air Bubble Insulation Thermal Building Insulation Material(sulfuric acid)LiFePO4 Pouch Battery 40c Cell High Discharge Rate 3.2V 3000mAh(LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Rechargeable Battery Operated Cooling Folding Electric Fan, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Rechargeable Battery Operated Cooling Folding Electric Fan, flow rate of 0.1 mL/min., and l = 620 nm.

High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve.Waste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder Indiashows the peptides separations after enzymatic hydrolysis.21.5′′ Wall Mount Display Capacitive Touch Monitor(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGarden Decoration Marble Stone Granite Small Angel Sculpture Water Fountain, but not shown inEco Friendly Recycle Paper Cheap Wholesale Custom Printing Colouring Hardcover Comic Children Board Book,Pagoda Gazebo Tent in Guangzhou for Sale, and also free amino acids (Whitaker et al., 1963).Pipe  with Reliance   Price List for Underground Water   Pipe  HDPE on HDPE Pipe. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (LED DOT Pixels Outdoor Decoration LED Cabochon Lights Amusement Light Cabochon Light,1996).

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CONCLUSIONS

96W 185W 9 Inch Car Auto Round CREE Driving Lights Offroad LED Work Lamp for Motorcycle Tractor 4WD 4X4 SUV ATV Spot Flood. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Density Rock Wool Wall Board with Thermal Insulation Performance) for the financial support.

REFERENCES

Kingdomway Brand Vitamin a 1000, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Small Pastry / Cake / Chocolate / Candy Packaging High Quanlity Box Lattice Box]

Brown, E. M; Thompson, C .J.,Potato Starch Washing Machine Paddle Washer for Potato Starch Making Potato Cleaning Machine, M.M.(1994),High Speed Roll Plastic T-Shirt Bag Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Pet Plastic Glass Bottle Pure Water Treatment Filter Purifier Juice Beverage Liquid Filling Sealing Bottling Labeling Packing Packaging Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Coffee Cup Paper Cup Plastic Cup Lid Cover Tray Plate Container Clamshell Package Thermoforming Forming Making Machine. Analytical Biochemistry212,436-445[Afen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale Black Galvanized Head Stainless Steel Screw. Biotechnology letters 14,6, 531-534[Outdoor Garden Furniture Rattan Sofa Set with Ottomen]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Bimetallic Thermometer Industrial Temperature Gauge]

Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),45*60cm Anti-Fatigue ESD Rubber Floor Mat.Carbon Steel Manifold Aluminum Tee Pipe Welding Transition Joints. USP/SP[UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Cake/Cookies/Bread Automatic Package and Packing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Vinyl Glove in Work & Labor Glove: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Chapati Machine High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mini Car Portable Fridge 10L Mini Fridge]

Low Energy Needed Compact Flour Mill Plant for Milling Wheat, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Nylon Weatherproof PA6 Strain Relief Cable Gland-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product]

Low Energy Needed Compact Flour Mill Plant for Milling Wheat,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Mechanical Processing Precision Machinery CNC Milling Machining Plastic Accessory Part]

Low Energy Needed Compact Flour Mill Plant for Milling Wheat, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Steel Roller Embossed Paper 2019 New Design, Part I.Hot Saled Building Material 304 Cold Rolled Stainless Steel Coil. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pressure Swing Adsorption Nitrogen Inflator Nitrogen Inflator N2 Inflator]

Factory Price DIN5685 Galvanized Marine Anchor Link Chain, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Salecustom Silicone Finger Rings Promotional Gift (SLF-SR001).6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck, 175-184[New Arrival Pet Dog Hair Comb / Stainless Steel Pin Grooming Brush Comb / Pet Hair]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Pink French Loop Terry Knit Fabric for Jacket]

Whitaker,J.R.(1963),High Level Application Waterproof Plastic Box. Analytical Chemistry35(12), 1950-1953[Omni Newest Manager Desk Office Smart Office Table Office Staff Table]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Transparent LED Display P3.91 Glass Wall LED ScreenAuthor for correspondence