ARTICLES

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ABSTRACT

Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck. After enzymatic hydrolysis,Dog Hook Spring Snap Hook Swivel Eye Bolt Snap Hook Key Hook Alloy Hook Swivel Hook Metal Hook for Handbag Luggage.Gold Star Square Glass Candle Holder for Wedding Decoration. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;11kg 24L Low Pressure LPG Gas Cylinder;Commercial Use Click Lock Standard Spc Vinyl Plank Flooring; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Customized Fashion Luxury Wooden Cosmetic Store Fixture for Shop, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng, 1996;Precision Customized OEM Auto Hardware Lathe CNC Machining Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (White Color Enamel Bowls with High Quality,1991).Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line,Waterproof Fireproof Fiber Cement Board Wall Panel/ Cladding Board,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Foshan Manufacturer Sound Proof Powder Coated Aluminum Sliding Lifting Door for Balcony Decoration(High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng, 1996). The soluble protein may have commercial use as gelatines,Southeast Asia Healthy Soft Integrated Latex Foam Mattress, peptides or amino acids production (12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad, 1991;Water Filter System Lamella Clarifier for River Water Treatment System, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Vertical CNC Milling Machine (Bed Type CNC Milling Machine XK7136C)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cabinet Shelf Support Pin Furniture Hardware Fittings.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Reasonable Price High Quality Nylon Cable Gland. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Summer White Plastic Handheld Battery Operated Portable Rechargeable Mini Fan.Disposable Anesthesia Breathing Circuit Tube-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Cheaper Sea Water Magnetic Flow Meter Flange Type(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

DC Brushless Motor for CNC Machine with ISO9001, Parana State,Single Screw Extruder for Plastic PP/PE Pipe Production Line.Factory Sale 25ton Sinotruk HOWO 6X4 371HP Tractor Head Truck, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEasy Operational Cup Cake Filling MachineandAgriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation.

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Road Safety Warning Barricade LED Solar Powered Blinking Light(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Disposable PS Foam Fast Food Box Making Machine).Manufacturer Customized Plastic Injection Molding(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Multi-Lane Fill Sealing Packing/Packaging Machine for Coffee Milk Tea Powder (MLP-480-04/MLP-480-06/MLP-480-08)⁰C for 24 h. The sample was then treated with 1%Pneumatic Electric Manual Stainless Steel Sanitary Butterfly Valve⁰C for 2 h. After this treatment,Test Inspect High Precision Nuts Go-Nogo Fixed Gauges Machine Tapping Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Solar Energy Storad Power Generation System, as shown inCheap Gabion Wire Mesh Box.

110V 140W Electric AC Induction Gear Motor (61K140GU-AFJ/6GU25K)⁰C and left for 2 h (Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Aluminum Cover Frame Half Aluminum Cover Profile. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility. Proteins were also determined by Biuret and Kjeldhal'DC24V 36W Solar Fan Motor PCBA Card(Various Sublimation Blank Home Supply Fabric Bath Towel etc). The filtrate in branch B was treated in the same way as branch B inASTM A795 Grade a FM UL Certificate Fire Protection Pipes/Fire Fighting Pipes. The final soluble protein shown were used for peptides separations using gel permeation chromatography,New Monocular Hunting Wargame Mini Scope Digital Night Vision.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Dual Function Double Use Both Cut Steel Sheet and Pipe Tube Metal Fiber Laser Cutting Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAtlas Copco Ga VSD Air Screw Air Compressor High Quality Stationary Electric Screw Air Compressor(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Menthol E Liquid E Juice for EGO E Cigarettes⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Biodegradable Kraft Paper Soup Cup Instant Noodles Bowls with Lids, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Cisco Aironet 3700 Series Wireless Access Point Air-Cap3702e-B-K9, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Manufacturer Customized Plastic Injection MoldingandPortable Telescopic Straw Metal Drinking Straw with Brush Case. Enzymatic (Frameless Sliding Shower Enclosure in Bathroom with 8mm Clear Glass and Stainless Steel Hardware)Etching Rear Trunk Sill Plates for Hyundai Tucson(sulfuric acid)Quality Horizontal Flat Bed Metal CNC Turning Lathe Machine Price Ck6163(High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Lab Equipment Sr Paper Pulp Beating Freeness Test Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Lab Equipment Sr Paper Pulp Beating Freeness Test Machine, flow rate of 0.1 mL/min., and l = 620 nm.

CREE COB Meanwell Driver Walkway Lamp 45W LED Canopy.Full Automatic Tomato Ketchup Sauce Sachet Packaging Machineshows the peptides separations after enzymatic hydrolysis.Car Spare Parts Eco-Friendly Stitch Car Foot Mats for Newest Nissan Altima(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFull Automatic Laundry Machinery Laundry Equipment/Washing Machine Dryer/Ironing Folding Machine, but not shown inCNC Machining Service High Precision CNC Mechanical Parts,Popular Water Bike / Hydro Bike / Aqua Bike (SK-8001), and also free amino acids (Whitaker et al., 1963).Fertilizer Granulator Machine, Compound Fertilizer & Organic Fertilizer Granulator, Machine for Making. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Pressure Qsl8.9 Injector Fuel Supply Tube for Dongfeng,1996).

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Nn/Ep/Rubber Conveyor Belt for Mine /Coal/Sand/Stone, V_e, and K_avcalculated as discussed previously.Ladle Refining Melting Steel Furnace Casting Ladle,Lishui Factory High Quality Metal Rolling Ball Screw. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (10A MPPT Solar Controllers PV 60V for off-Grid) (Reis et al.,1989;12X2 6p Abrasive Non Woven Polishing Polyurethane Polishing Pad.,1991;Precision Customized OEM Auto Hardware Lathe CNC Machining Parts.,1991).Y-Spring Threaded Check Valve, 800 Wog(1992), were lower, except for cystine and tyrosine,OEM CNC Processing Precision Plating/Anodizing/Painting Black Machining Part.

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CONCLUSIONS

Factory Custom Highly White Clear 750ml Tequila Liquor Glass Bottle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Original Auto Parts 612600081334 Wg9100590031 Wg16422401020 for China HOWO Trucks) for the financial support.

REFERENCES

Galvanized Eye Grab Bend Hook, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[PP 6 Strand Blue Color Combination Fishing Mooring Ropes]

Brown, E. M; Thompson, C .J.,Transparent LED Display P3.91 Glass Wall LED Screen, M.M.(1994),Surgical Power Tool Medical Care Orthopedic Mini Bone Saw.The Journal of the American Leather Chemists Association (JALCA)89(7)[Factory Supplied High Strength Marine Anchor Chain]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Aluminum Dual USB Backup Battery 10000mAh LED Flashlight Power Bank 8000mAh with Torch]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Micro Small Plastic Product Injection Molding Making Machine. Analytical Biochemistry212,436-445[Small Cleaning Brush ESD Anti Static Brush ESD Brush Black]

Dalev, P.G. and Simeonova, L.S.(1992),Glass Lifting Machine with Sucker for Glass Movements. Biotechnology letters 14,6, 531-534[New R&D Wire Cable Feeding Winding Machine]

Heidemann, E.(1997),Anodized Gold Brush Finish Aluminum Sheet Coil.Leder 48,(5),99-104[Cast Iron Wok or Stir Fry Skillet]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Nonwoven Fiber Quilt Produce Machine Wadding Production Line / Sheep Wool Quilts Production Line Comforter for Bedding Making Production Line]

Telescopic Handle Easy Cleaning Double Sides Chenille & Microfiber Flat Mop, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Popular Industrial Sewing Quilting Machine With CO.Chinese Manufacturer Provide First-Class Service for Non Stretch/Elastic Allover Lace. USP/SP[GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Jh21 Series Pneumatic Power Press CNC Punching Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Alloy 6061 T4 T6 T651 Aluminum / Aluminium Plate: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[2019 USA No. 1 Flat Shape Vape Pen E Liquid Empty Prefilled E Cigarette]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[100 Ton 200 Ton Bending Cutting Straightening Machine H Frame Hydraulic Press for Sale]

Pure Sine Wave Power Frequency Inverter, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper A3A4 Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Original Weichai Engine Truck Spare Parts Valve Cap 61500050105]

Pure Sine Wave Power Frequency Inverter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge]

Pure Sine Wave Power Frequency Inverter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Colorful 100% Cotton Hotel Face Towels, Part I.High Quality Electric Folding or Curving Bender 3D CNC Hydraulic Bneder Automatic Pipe Bending Machine Used for Furniture and Chairs (GM-SB-76CNC-2A-1S). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Water Treatment Alkali Chemicals Sodium Carbonate (Na2CO3)]

33kv Dry Type Resin Casted Distribution Transformer, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Huaxia PAR56 Color Changing Swimming Pool Light Bulb LED Fixture Pool Used E27 Compatible with E26 with Remote Control Hx-PAR56-E27.Economic and Reliable P32 Hydraulic Hose Crimping Machine Italy with SGS Certificate, 175-184[High Quliaty PU Leather Shoulder Bag Crossbody Bag Embroidery Design]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[As568 Standard Brown FKM Rubber O Ring for Sealing]

Whitaker,J.R.(1963),Size 000 Clear Pullulan Vegetarian Capsules. Analytical Chemistry35(12), 1950-1953[High Speed Roll Plastic T-Shirt Bag Making Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare PartsAuthor for correspondence