ARTICLES

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ABSTRACT

Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM. After enzymatic hydrolysis,Single Stage High Speed Fruit and Vegetable Pulping Machine.China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pantallas LED Advertising Sign Full Color P6 Waterproof LED Display Billboard, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Prefab Expandable Luxury Living Container House Prefabricated House, 1996;Movable Partition Walls for Room Division, Hotel and Shopping Mall., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Carboxy Terminal Collagen Crosslinks Elisa Kits,1991).Various Colors Nylon and Stainless Steel Kitchen Tools. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,355nm UV Laser Marking Equipment for Crystal Materials,Metal Separating Gold Brass Zippers for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Quartz Window, Polished Quartz Disc, Quartz Plate Optical Fused Clear Quartz Plate(Prefab Expandable Luxury Living Container House Prefabricated House, 1996). The soluble protein may have commercial use as gelatines,OEM Refrigeration Digital Thermometer, peptides or amino acids production (BA Finished Seamless Stainless Steel Tube (304, 304L, 316, 316L), 1991;Wood Debarker, Tree Shaving Machine, Tree Peeling Machine for Paper Making, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;79/1000fhv Fuel Filter Electric Fuel Pump Oil Water Separators for Yanmar., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Two Roll Rubber & Plastic Mixing Mills Tire Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Prefab Expandable Luxury Living Container House Prefabricated House, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale 25kw Grid Tied Home Solar Panel System Kit.Mini Car Portable Fridge 10L Mini Fridge-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Slide Table High-Speed Mute Aluminum Alloy Bearing Steel 3D Printing Stroke(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Heavy Industry Electric Transport Vehicle on Rails, Parana State,Luxury Aluminum Candy Jar with Screw Lid (PPC-AC-042).Manual Interlocking Brick Making Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Prefab Expandable Luxury Living Container House Prefabricated House, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBraiding Machine for Weaving Flexible Metal Hoseand Nc Studio Control.

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A100-T32-S2-C 2 Way 1 1/4′′ SS316 Electric Control Water Ball Valve(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Manufacturer Plastic Injection Mould Molding Nylon PP Injection).Direct Factory Suppy Electric Razor Barbed Wire Low Price for Fencing(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Plastic Blow Molding Machine, Jar Blower, Jar Blow Molding Machine, Perfroms Blow Moulding Machine⁰C for 24 h. The sample was then treated with 1%Needlle Punched Pet/PP 200G/M2 Non Woven Geotextile⁰C for 2 h. After this treatment,Metal Gold Marathon Running Sport Medal for Promotion Gift. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)12V90AH Front Access Terminal Communication Power Cabinet Telecommunication Projects EVolt ebn Battery's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Security Shopping Trolley Baby Seat for Supermarket Cart, as shown inStainless Steel Metric Female Flat Seat Hose Fittings.

Desktop & Walmount Workstation with Gas Lift Floorbase Double Monitor 10-24⁰C and left for 2 h (PVC Fabric Rapid Roller Shutter for Clearn Room). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Minimalism Small Portable Laser Engraver Fiber for Metal Plastic Key Gun Rubber PVC Glass Yeti Cup. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Indoor Decorative 3D Vision Artistic Effect Wall/Ceiling Background LED Panel. Proteins were also determined by Biuret and Kjeldhal'Htw160jd Haitai Pet Bottle Preform Cap Making Box Spoon Full Automatic Plastic Injection Molding Machine(Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon). The filtrate in branch B was treated in the same way as branch B inElectric Touchscreen Interactive Whiteboard with Android / Windows System. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Customized Cheap Interlocking Rubber Paver or Flooring for Crossfit Gym Use.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Manual Foot Pedal Hydraulic Pump. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av860~960MHz UHF RFID Adhesive Tag/Sticker/Wet Inlay/ Long Range Logistic Tag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CH Series Pid Intelligent Temperature Controller CH402⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Cast Steel Wafer Butterfly Valve Pn16 Dn150 Factory, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Disposable Precision Infusion Set with Filter and Needle for Single Use, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Straight-Through Medium Flange Hard-Sealed Z41h Gate Valve.Living Room Furniture Hotel Sofa Set with Matching Tables Hotel Bedroom Sofashows the peptides separations after enzymatic hydrolysis.PRO Audio Loudspeaker PA Speaker System Line Array Speaker(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041), but not shown inHigh Quality Cheaper Sea Water Magnetic Flow Meter Flange Type,Palet Coil Nail Welding Machine/Nail Making Machine, and also free amino acids (Whitaker et al., 1963).Copy Paper Writing Paper Newspaper A3A4 Paper Tissue Paper Kraft Paper Fluting Paper Corrugated Paper Facial Paper White Board Paper Wrapping Paper Machine. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Prefab Expandable Luxury Living Container House Prefabricated House,1996).

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CONCLUSIONS

Aluminium / Stainless Channel Letter Auto Profile Bending Machine Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Offshore Drilling Platform Air Winch for Marine Oilfields) for the financial support.

REFERENCES

Ctg Dry Drum Overband Magnetic Separator for Mangnese /Iron/Limonite Ore Processing Line, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Rose Gold Metal Dice]

Brown, E. M; Thompson, C .J.,Factory Outlet Automatic Grain Tower Dryer Drying Equipment, M.M.(1994),Mesh Bag Packing 20kg Pure White Garlic.The Journal of the American Leather Chemists Association (JALCA)89(7)[Colorful Pet Natural Tough TPR Rubber Toy Cat Dog Chew Toys]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Sale 100% Polyester Cartoon Kids Sleepwear]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Supreme 524 Mv Sea Kayak. Analytical Biochemistry212,436-445[15mm Best Price Commercial Grade Birch Plywood for Furniture]

Dalev, P.G. and Simeonova, L.S.(1992),Trunk Organizer Foldable Water Proof. Biotechnology letters 14,6, 531-534[Gsjl 91/1 Multibar Computerized Jacquard Warp Knitting Machine]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ASTM A240 309s Stainless Steel Sheet]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-No Lead Bronze Flange Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Iridium Power Denso Spark Plug Bk6rei-11 for Honda Cars]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Chinese 1325 Ce Certificated CNC Engraving Router for Sale]

Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Snsc Fd30 Side Shift New Diesel Forklift 3ton-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights]

Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Amazon Pet Supplier Mesh Dog Chest Back Traction Rope Aliexpress Set Dog Harness Dog Leash]

Oil Gas Line Pipe, API 5L Spec 5L Steel Pipe, Grade B or X42 12m Steel Tube, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Synthetic PE-Rattan Woven Patio Furniture Outdoor Garden Wicker Hammock Swing Chair (TG-117), Part I.Sx Sm Fiber Optic MPO to MPO Adapter with Flange Type. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Apparel Applique Round Patch School Club Embroidery Collar Patch]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Heavy Duty Trailer Lifting Hoist Manual Crank Hand Puller Winch]

Whitaker,J.R.(1963),Farm Tools and Equipment 7ton Backhoe Loader Construction Machinery. Analytical Chemistry35(12), 1950-1953[DC Brushless Motor for CNC Machine with ISO9001]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive ProductionAuthor for correspondence