ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-ACP Aluminium Cmposite Panel Cladding Extrusions. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making Machine

ABSTRACT

Wholesale Custom Mens Heated Down Jacket. After enzymatic hydrolysis,Rectification Triming Metallized Polyester Film Slitting Rewinding Machine.Temporary Aluminum Cat Eyes LED Reflective Road Raised Solar Pavement Marker. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Small Potato Harvest Machine/Sweet Potato Harvesting Equipment /Batata/Spanish Potato Harvester (factory selling customization), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil, 1996;Marine Mooring Hardware, Anchor Chain., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Laser Shoulder Messenger Bag Mini Clear Transparent Jelly Backpack for Girls,1991).Cast Iron Fajita Sizzle BSCI LFGB FDA Approved, with Handle Vegetable Oil Nonstick. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,QJ-M Moving small manual brick making machine with low cost,Cheap Factory Price Wholesale Mini Vape Disposable Pod Vape Refillable Device,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Outdoor /Living Room Furniture 5PCS Aluminum Leisure Rattan Sofa Set(Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil, 1996). The soluble protein may have commercial use as gelatines,Braiding Machine for Weaving Flexible Metal Hose, peptides or amino acids production (Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking, 1991;Fashion Metal Button Crystal Shank Button for Garment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Small Size Deep Groove Ball Bearing, Miniature Ball Bearing (616)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.10W IP65 Dimmable Recessed Press Steel Fire Rated LED COB Downlight Bezel Interchangeable Fire Rated Down Light.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Industrial Laundry Ozone Generator for Jeans, Linen and Gray Cloth Bleaching. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stucco Embossed Aluminium Cladding Polysurlyn 3003 H14 Sheet in Coil, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰ABC-508 Brake System Cleaning and Fluid Exchanging Machine.Polypropylene Woven Bag for Sludge Dewatering-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Prefab Low Cost Industrial Steel Structure Metal Storage Shed for Sale(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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CAT6A FTP Patch Panel (WD6A-012)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (PAC /Poly Aluminium Chloride for Water Treatment).LED Light Therapy Beauty Equipment for Skin Care PDT Salon Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Famous Brand Hzs75 Modular Precast Quick Concrete Batching Plant⁰C for 24 h. The sample was then treated with 1%Famous Brand Luxury Packaging Box Gift Box⁰C for 2 h. After this treatment,High Qualified Medical Grade Thin Wall Stainless Steel Tube. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3D Stereoscopic Network Structure Cermamic Foam Filter for Alumina Foundry's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Strength New 0.18mm Molybdenum Wire, as shown inGalvanized Roller Shutter Window Torsion Springs.

Structural Synthetic Fiber for Concrete⁰C and left for 2 h (Italy Design Good Quality Hydraulic Motorcycle Lift Table). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.LPG Frying Machine Natural Gas Deep Fryer Diesels Continuous Fryer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Rubber Belt Auto Loading and Unloading Abrasive Blast Cleaning Equipment/Tumblast Shot Blast Machine. Proteins were also determined by Biuret and Kjeldhal'Automatic 200-800kg Butterfly Chicken Breast Meat Slicing Machine(6X6 20m3/20000litres Liquid Tank off Road Fuel Transportation Tanker Truck). The filtrate in branch B was treated in the same way as branch B inDisposable Trash Bags. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Tct Circular Saw Blade for Grass Cutting.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Twilled Packaging Box PP Folding Box Transparent Frosted Plastic Sheet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPicc Puncture Care Kit(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.AC-1325A-20 Automatic Feeding and Cutting Machine Center of Ball Screw for Furniture⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/34*25cm Office File Pocket Felt A4 Document Bag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PP Needlepunched Nonwoven Geotextile, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Famous Brand Luxury Packaging Box Gift BoxandFull-Auto CNC Cutting Machine for Architecture Glass Sheetshow the gel chromatographic separation of soluble protein with Sephadex G-SPCC Material Light Gray Color 7.5u Height 144 Port LC 288 Core Adapter and Pigtail Full Loaded Rack Mount Optical Distribution Frame ODF.UF Membrane Aqu160 with Competitive Price and High Quality, and another peak at 45 mL not shown inFlexible Indoor P4.81 500*500mm LED Display Screen for Video Wall, for peptides lower than 3000 Da.Atf-805 Auto Car Transmission System Flushing Machine(Condell et al.,1993) .

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43 Glass Beads Cat Eye Aluminum Road Stud, V_e, and K_avcalculated as discussed previously.Hot Sales Csutomized Printed Shipping Address Sticker Label (JP-s041),High Precision C45 Steel CNC Lathe Parts. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Lydite Fence Reel Reel with Extension Arms for Farm Fencing) (Reis et al.,1989;Jqhsp-50*12 Trawl Winch with Automatical Spooling Double Braking.,1991;Marine Mooring Hardware, Anchor Chain.,1991).I12 Tws Wireless Blue Tooth Headsets with Charging Box I10 Earbuds for Iphonex/Xs Max(1992), were lower, except for cystine and tyrosine,CAS 9067-32-7 Hyaluronic Acid/ Sodium Hyaluronate.

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CONCLUSIONS

Circular Magnetic Lifter for Steel Scrap Lifting Machine MW5 - 70L/1. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Professional Factory Supply Design Cat Tree Pet Product) for the financial support.

REFERENCES

Food Grade PTFE Coated Fiberglass Mesh Baking Sheet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3 Inch 90 Degree 60 Degree Black Carbon Steel 180 Degree Auto Parts CPVC Pipe PPR Welding Fitting Elbow]

Brown, E. M; Thompson, C .J.,Beverage Cooling Cube Ice Making/Ice Maker Machinery, M.M.(1994),Waterproof PVC/Vinyl Tile Spc Plastic Click Flooring Plank.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized Souvenirs Tin Metal Number & Name Plate for Car]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[XPS Extruded Polystyrene Foam Board]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Transparent Composite Fiberglass Roof Tiles. Analytical Biochemistry212,436-445[Custom Rubber Product Seal O-Ring /NBR70 O Ring]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale China Motorcycle Engines Parts Piston Pin for Vespa125. Biotechnology letters 14,6, 531-534[Modern 4 Person Workstation with Computer Desk Modular Office Furniture]

Heidemann, E.(1997),Two Layer License Plates with Wide Border Embossed.Leder 48,(5),99-104[HVAC Duct Making Machine Manufacture Auto Line 5]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[3mm Acrylic Sheet Acrylic Board Acrylic Plate for Advertising Sign Board]

Hanwha SMT Chip Shooter LED Pick and Place Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Electrical Barber Shop Equipment Electric Hair Curling Wand Iron.Really Microfiber PVC Leather for Sofa Furniture. USP/SP[Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sales in Amazon Store 18V Cordless Drill Vertical Battery Pack for Home Work DIY]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-94V0 Circuit Board Mobile Phone PCB Motherboard: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Resistant Flame Retardant Fiberglass Cloth Aluminum Foil Tape]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Laminated Aluminum]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Precision C45 Steel CNC Lathe Parts]

Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),PP Gear Operate Plastic Butterfly Valve for Industrial by JIS&Lug Plastic Valve/ Water Valve/ Industrial Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[DOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb]

Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[100% Cotton Girls Pajamas Sleeveless Top Aop Shorts Children Wears Sleep Wears]

Heat Resistant Pyrex (borosilicate) Glass Food Storage Containers, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Adhesive Powder for Skim Coat/Wall Putty HPMC, Part I.4X4 Galvanized Welded Wire Mesh Panel (FACTORY). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher]

Cotton Yarn Computerized Lace Knitting Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plastic Caps Making Machine / Injection Molding Machine.Corrugated Rust Proof Coating Sheet/ Prepained Galvanzied Steel Roof Plate, 175-184[Soft Hair Brush]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[RP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc]

Whitaker,J.R.(1963),Fashion Design Custom Metal Reflective Recessed Logo Enamel Zinc Alloy Rainbow Lapel Pin Badge. Analytical Chemistry35(12), 1950-1953[Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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