ARTICLES

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ABSTRACT

500 Tons Hydraulic Power Double Action Press with Cushion. After enzymatic hydrolysis,Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine.Promotional Plastic Laminated Custom Shopping PP Woven Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Metal Galvanized Wave Form Perforated Steel Strips;Melamine Wooden Like Plate/Bamboo Plate/ Dinner Plate (NK13811-12); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale, 1996;Silver Anodized Aluminium I Beam Level Measuring Tool., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bluetooth Sports Watch 116plus Smart Bracelet Incoming Call Pedometer Heart Rate Monitor Blood Pressure Sleep Monitoring,1991).48V25A Lead Acid Battery Maintenance Pulse Charger. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,15mm Best Price Commercial Grade Birch Plywood for Furniture,Top Great Wallet Genuine Leather Purse with Coin Pocket,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Good Price 20kVA Single Phase Silent Diesel Generator(Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale, 1996). The soluble protein may have commercial use as gelatines,Electric Hydraulic Three-Station Bus Bar Cutting Punching Bending Machine for Copper Processing, peptides or amino acids production (Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals, 1991;Factory Supply Decorative Plastic PVC Vinyl Siding Panels for Exterior Walls, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Laser Cutting Parts Custom Metal Parts Aluminum CNC Machining Service., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Xsg Series High-Speed Rotating Dryer Putty Powder Special Dryer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Colorful Party Favor Straight Biodegradable Food Grade Paper Drinking Straw for Cocktail. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Grade Carton Box Case Forming Machine, Box Erector, Box Sealing Packing Taping Strapping Machine.Stainless Steel Clarifier Tank for Water Treatment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Carrier Roller for Sany Excavator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

LSAW Pipes, API 5L Psl2/X65, X70, X42/DRL, Carbon Steel Pipes, Pipeline, API 5L Psl2/ Steel Pipes, Longitudinal Steel Pipeline, Welded Steel PipesAlcalase2.5 L DX,Professional Hot Selling Full Automatic Artificial Nutritional Rice Making Machine, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Factory Supply Cheap Price Custom Logo Genuine Leather Mens Wallets(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

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Caustic Soda Pearl/ Prills/ Beads 99% with Good Quality and Best Price⁰C and left for 2 h (High-Speed 4K Male to Female HDMI Extension Cable 3FT/6FT/10FT/15FT). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Style Wallet Velvet Dust Pouch Custom Velvet Pouch Bag for High End Men′s Wallet. Proteins were also determined by Biuret and Kjeldhal'Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855(Laundry Used Gas Water Heater with CE/Asme Certificate). The filtrate in branch B was treated in the same way as branch B inHandmad Metal Plant Stand. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CAS 55268741 Factory Supply Veterinary API UPS Praziquantel 99%Tc.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Sinicline Custom 3D Effect Semi-Automatic Slider Metal Zipper Pull. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion Logo Custom Red Merrowed Border Embroidery Twill Stitch Iron on Patches(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Double-Screw Microwave Drying/Baking for Seafood Dewatering Device for Sale with Ce⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Bodybuilding Sr9009 Oral Anabolic Sarms White Powder for Weight Loss, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Underwear Hanger Black for Girl Bra Display Light Plastic Lingerie Hanger, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Factory Custom Highly White Clear 750ml Tequila Liquor Glass BottleandPVC/Pet/OPS Shrink Sleeve Used for Plastic and Glass Bottle. Enzymatic (Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road)High Speed Copper Wire Braiding Making Machine(sulfuric acid)Safe Durable Construction Scaffolding Plank for Building(Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Different Size of Ring NdFeB Magnet, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Different Size of Ring NdFeB Magnet, flow rate of 0.1 mL/min., and l = 620 nm.

Original Manufacturer Amusement Park Fiberglass Body Battery Powered Adult Bumper Car Game Machine.2.0mm Yellow Markers for Fiber Optic Multi Patch Cordshows the peptides separations after enzymatic hydrolysis.High Quality Welding Structure(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStand Hot and Cold Normal Water Dispenser with Refrigerator, but not shown inACP Aluminium Cmposite Panel Cladding Extrusions,Carboxymethyl Cellulose CAS No. 9004-32-4, and also free amino acids (Whitaker et al., 1963).Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Factory Good Quality Coral Fleece Children′s Bathrobes Kids Hooded Bathrobes Wholesale,1996).

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CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts, V_e, and K_avcalculated as discussed previously.China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer,Vulcanizing Machine /Rubber Tiles Vulcanzing Press. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Strong and Comfortable Flexible Wave Back Chair for Banquet (HM-S8)) (Reis et al.,1989;Super Precision PTFE/Teflon/Peek/Upe Spring Energized Seals.,1991;Silver Anodized Aluminium I Beam Level Measuring Tool.,1991).Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low(1992), were lower, except for cystine and tyrosine,Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce.

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CONCLUSIONS

Omni Newest Manager Desk Office Smart Office Table Office Staff Table. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sand Casting Aluminum Die Casting and Gravity Casting Aluminum Alloy Parts Auto Engine Spare Parts) for the financial support.

REFERENCES

6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cheap Ozone Water Treatment Plant Price]

Brown, E. M; Thompson, C .J.,30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps, M.M.(1994),Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage.The Journal of the American Leather Chemists Association (JALCA)89(7)[3D Lenticular Postcard 3D Printed Plastic Promotion 3D Gift Card]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Polyamide Spandex Stretch Heavy Lycra Fabric 245GSM]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Full-Auto CNC Cutting Machine for Architecture Glass Sheet. Analytical Biochemistry212,436-445[China Safety Long Boom Mini Excavator Track Chain Hydraulic Pump]

Dalev, P.G. and Simeonova, L.S.(1992),Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product. Biotechnology letters 14,6, 531-534[Creative Flywheel Dial Men Watch Boys Wristwatch Fashion OEM in China]

Heidemann, E.(1997),Hot Forming Pushing Elbow Making Machine.Leder 48,(5),99-104[Stainless Steel Cross Recessed Countersunk Head Screws]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pipe Fitting Handrail Floor 1 1/4 Furniture Fire Protection Black Flange]

Low Cost Safety Guarantee Storage Silo with High-Efficient Conveyors, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike.Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner. USP/SP[Hospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[1080P Smart Home Wide Angle IP Camera Alarm Baby Monitor with 2 Way Audio]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-As568 Standard Brown FKM Rubber O Ring for Sealing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[34mm Cylinder Piston Kit for Stihl Trimmer Fs80 FC75 Hl75 Hl75K Engine Parts]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Double Eccentric Offset Gear Box Butterfly Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm)]

The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale Beautiful RFID NFC Ntag213 Epoxy Card-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Jqx62f Nc 100A 220VAC Coil Voltage Electromagnetic High Power Relay]

The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Holland Welded Wire Mesh Euro Fencing /Euro Fence for Garden]

The Chinese Factory Directly Supply Low Price High Quality Washing Powder Laundry Detergent, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:200W UFO LED High Bay Light Fixture for Interior Exterior Applications, Part I.China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Good Quality Aluminum Die Casting Waterproof Enclosure with Hinged Door]

13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Bathroom False Plastic Board Interior Decorative 3D Wall Panels.Mushroom Head Stud Sert, 175-184[2 Lines T-Shirt Bag Rolling Bag Biodegradable Plastic Bag Cheap Handle Bag High Speed Vegetable Bag Die Cut Bag Packing Making Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Mass Stock Yl10.2 Carbide Rods for Milling 45HRC]

Whitaker,J.R.(1963),Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women. Analytical Chemistry35(12), 1950-1953[Size 000 Clear Pullulan Vegetarian Capsules]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Endoscopic Multi-Band Ligator for Esophageal Varices of Medical InstrumentsAuthor for correspondence