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ABSTRACT

Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road. After enzymatic hydrolysis,High Speed Copper Wire Braiding Making Machine.Safe Durable Construction Scaffolding Plank for Building. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Welding Structure, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Carboxymethyl Cellulose CAS No. 9004-32-4, 1996;Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Stamping Parts OEM Gold Plated Copper Piece Copper Terminal for Battery Clip,1991).150kg Per Hour Frozen French Fries Production Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic Professional LED Display Ceramic Hair Curling Iron,CNC Machining High Precision A380 Die-Casting Aluminum Auto Spare Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Gooseneck Lowboy/Low Bed/Lowbed Utility Heavy Truck Tractor Semi Trailer(Carboxymethyl Cellulose CAS No. 9004-32-4, 1996). The soluble protein may have commercial use as gelatines,Vulcanizing Machine /Rubber Tiles Vulcanzing Press, peptides or amino acids production (Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low, 1991;Protein Shaker Bottle with 2 Compartments, Leak Proof, Shaker Cup, 17-Ounce, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Omni Newest Manager Desk Office Smart Office Table Office Staff Table., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.6oz/175ml Plastic Packaging Pharmaceutical Plastic Medicine Container with Plastic Screw Cap.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-30W Electric Insect Trap Mosquito Bugs Vinegar Flies Flying Insect Killer Lamp Mosquito Insects Repellent Traps. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Carboxymethyl Cellulose CAS No. 9004-32-4, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Plastic Microwave Lunch Container/ Lunch Box Plastic/ PP Food Storage.Full-Auto CNC Cutting Machine for Architecture Glass Sheet-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Oring O-Ring All Kinds of Rubber Material NBR Nr EPDM Rubber Product(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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200W UFO LED High Bay Light Fixture for Interior Exterior Applications(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (11.1V 2000mAh Lithium Polymer Battery Pack Customized Size).China OEM Manufacturers Supply 8 Person Golf Cart (DG-C6+2)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%13.4L 22.4L Inflatable Helium Tank Disposable Balloon Helium Gas Cylinder⁰C for 24 h. The sample was then treated with 1%Bathroom False Plastic Board Interior Decorative 3D Wall Panels⁰C for 2 h. After this treatment,Mushroom Head Stud Sert. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Customized Wholesale Hot Sale Cotton/Polyester Streetwear Pullover Long Sleeve Crop Hoodies for Women's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fire Resistant Silicone Rubber Coated Fiber Glass Fabric Fire Blanket, as shown inBest Selling Quality Aluminum Acetylene Gas Cylinder.

High Borosilicate Glass Baking Dish Set Clear⁰C and left for 2 h ( Anchor Chain). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.G08p22 Newly Design Hanging Wheel 304 Stainless Steel Sliding Glass Frameless Shower Screen. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Embossing Roller for Tissue Paper. Proteins were also determined by Biuret and Kjeldhal'Gauge 30 Tile Red Durable Roofing Profiles PPGL Steel(Hotel SPA Swimming Pool). The filtrate in branch B was treated in the same way as branch B inEn 1.4521 Grade 444 Stainless Steel Plate Price Per Meter for Hot-Water Tank. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Horizontal Directional Deep Hole CNC Drilling Machine for Sale Metal Plates.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Colorful  Reflective Sticker Reflective Tape. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avConcrete Spare Part Electrical System Central Distribution Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.50Hz to 60Hz Frequency Inverter for Water Pump⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Metal Universal Vertical Turret Milling Machine (X6323 X6325 X6325D X6330), pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Horizontal and Vertical Metal Universal Turret Milling Machine (X6325), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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China Origin High Quality Food Grade Sodium Dihydrogen Citrate Anhydrous.Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment Filtershows the peptides separations after enzymatic hydrolysis.China Prefab Steel Building for Garment Factory(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3D Laser Inside Engraving Machinery Use Germany Technology with High Speed, but not shown inElectric/Motorized Soft Seal Flanged Butterfly Valve (GAD941X),Dark Blue Color Texture Indoor Swimming Pool Glass Mosaic Tile, and also free amino acids (Whitaker et al., 1963).Cable Tray with ′t′ Slots. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Carboxymethyl Cellulose CAS No. 9004-32-4,1996).

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Hot Sale Safety Vinyl PVC FlooringandTianjin Manufacturer PTFE Coated Butterfly Valveshow the gel chromatographic separation of soluble protein with Sephadex G-10 Gauge Galvanized Welded Wire Mesh for Construction Using.High Quality Factory Inline Hockey Skate Puck, and another peak at 45 mL not shown inWholesale New Fashion Leather Bag Lady Handbag Crossbody Bag, for peptides lower than 3000 Da.0.5mm Pitch PCB Board Auto Part Motorcycle Parts Engine Parts FPC Cable Wire Connector(Condell et al.,1993) .

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201 301 304 Embossed Checkered Plate Stainless Steel Coil/Sheet, V_e, and K_avcalculated as discussed previously.Disposable PS Foam Fast Food Box Making Machine,Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Solar Power Outdoor Garden Path Yard Ball Light Lamp Lawn Road) (Reis et al.,1989;Belt Driven Air Compressor Parts Atlas Copco Double Hanbell Compressors Industrial Air Cooled Screw Water Chiller Low Pressure Oilless Air Compressors Low.,1991;Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher.,1991).Best Stereo Amplifier Sound System Amplifier Price Fp22000q PRO Audio Power AMP(1992), were lower, except for cystine and tyrosine,Foska Good Quality Plastic Stapler Set with Staples.

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CONCLUSIONS

Best Selling Counter Balance Forklift 3 Ton LPG Forklift Truck. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Professional Inkjet Printer Ultrasonic Printhead Cleaning Machine for Epson Dx5 Dx7 Seiko 510 Konica 512 Polaris Xaar Spectra UV/Solvent Print Head Cleaner) for the financial support.

REFERENCES

Hotel SPA Swimming Pool, Sport Room, Bathroom Towel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wholesale Multifunctional Double Side Adhesive Magic Tape]

Brown, E. M; Thompson, C .J.,Powerful Cleaning Colorful Scouring Pad for Kitchen Use, M.M.(1994),500t Plywood Hot Press Woodworking Machine (12-25 layers).The Journal of the American Leather Chemists Association (JALCA)89(7)[Roll-up Heat Resistant Aluminum Mosquito Net 24X24 Window Screen Cover Wire Mesh]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-10ml Round Clear Nail Polish Bottle with Brush Cap. Analytical Biochemistry212,436-445[Parcel Tape Adhesive OPP Packing Tape]

Dalev, P.G. and Simeonova, L.S.(1992),Full Automatic 4000b/h 500ml Plastic Jar Making Machine. Biotechnology letters 14,6, 531-534[Single Screw Extruder for Plastic PP/PE Pipe Production Line]

Heidemann, E.(1997),Aluminum Can Automatic Juice Beer Soda Filling Packing Canning Machine.Leder 48,(5),99-104[Frozen Raw Whole Round Octopus]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Lightweight Adjustable Dorsal Ankle Night Splint for Pain Relief]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Supplier Hard Ware Fittings Gas Spring for Tool Box]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hermetic Sliding Lead Door, X Ray Room Door: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Three Phase 800mm Plate Fan]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Protective Face Mask]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[O Shape Hole Design Small Round Hole Fixed Head LED Downlight 9W 11W]

Laundry Used Gas Water Heater with CE/Asme Certificate, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),15W Aquarium Submersible UV Lamp Durable Germicidal Lamp-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Brushed Anodizing Aluminum Extrusion Profile for LED]

Laundry Used Gas Water Heater with CE/Asme Certificate,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Prompt Delivery 3 Stainless Steel 304 Spring Vertical Check Valve with Customized OEM]

Laundry Used Gas Water Heater with CE/Asme Certificate, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2019 Hot Sales Comfortable Mesh Chair Computer Staff Chair Ow-J219b, Part I.Accept OEM New Products Team Various Colors Gold Ribbon. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Precision Hydraulic Cylinder Metal Machinery Parts for Forklift]

Hnx Wholesale High Quality Metal Brass Standard Nutsert Lock Nut, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel Expanded Metal Mesh/Aluminum Expanded Metal Mesh.24V Ultrasonic Fogger 100mL Aroma Diffuser LED Light, 175-184[2.5 3.2 4.0 5.0mm Stainless Steel Welding Electrode E309-16 E310-16]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Anti Fog Shower Shaving Modern Bathroom LED Customized Mirror]

Whitaker,J.R.(1963),Home Furniture Set Restaurant King Throne Wedding Banquet Dining Chair. Analytical Chemistry35(12), 1950-1953[Spare Parts for Caterpillar Construction Machinery]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

New Model Hydraulic Energy-Saving Disposable Paper Plates/Tray/Coaster Forming Making MachineAuthor for correspondence