ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Diameter 1300mm Lifting Electro Magnet for Cast Iron. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High Purity Magnesium Metal Ingot/Pure Magnesium Alloy Ingot 99.98% 99.95%

ABSTRACT

Evaporator Finned Tube. After enzymatic hydrolysis,Men Training Sportswear Collection Muscle T Shirt Function Vest Fitness Shirts Sport Tops Gym Activewear.Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CNC Turning Center, CNC Horizontal Turning Milling Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16, 1996;Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Classical Garment Accessories Jeans Button Metal Shank Button,1991).Vitamin D3 Fat-Solubility Water-Solubility Feed Additive. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Factory Wholesale Zinc Plated Carbon Steel Hex Nut,Prepainted Color Coated 3003 PVDF Aluminium Steel Coil with Wooden Pattern,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized Electric Vehicle Brushless DC Motor 1kw 3000rpm 24V(Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Cheese Making Machine Electric, peptides or amino acids production (Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts, 1991;100W Dimmable LED Corn Bulb with Meanwell Driver, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pet and Animal Food Extrusion Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory Various Spi /I2c /0.5-4.5V /4~20mA Air Water Gas Pressure Sensor Transducer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hexagonal Wire Mesh Chicken Wire Mesh High Quality. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Factory Hot Sale Decorating The Christmas Tree Polyester Stain Ribbon Pmb-16, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰V-32 New Special UPS Solar Battery Cabinet Box Case.4-14 Manual Staple Gun with Staples for Hand Tools-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Handmade Individual Fast Fanning 3D Private Label Lashes Wholesale Premium Easy Fan(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Microfiber Magnetic Custom Bath Set 100% Printed Kitchen Tea Towels(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China Modern Pharmacy Vacuum Filter Press).15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Aluminum Die Casting Waterproof Junction Box IP67 Aluminum Outdoor Enclosure⁰C for 24 h. The sample was then treated with 1%80W All in One Outdoor LED Street Light Solar Lamp⁰C for 2 h. After this treatment,Hot Sale Safety Vinyl PVC Flooring. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Agriculture Farmland Refractive Atomizing Greenhouse Micro Sprinkler Irrigation's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Horizental Stainless Steel Food Grade Milk Tank for Milking, as shown inFolding Play Mat Crawl Play Mat.

Multi Parameter Small Digital Domestic Water Meter TDS Meter⁰C and left for 2 h (Dbry-320 Christmas Stickers Labels Printing Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.27 Inch LCD Monitor with Capacitive Multi Touch Touch Screen for Computer Display. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sanhe FC-2 Poultry Air Vent. Proteins were also determined by Biuret and Kjeldhal'Picosecond Laser Tattoo Removal Skin Care Medical Instrument(High Density Celuca Kitchen Cabinets PVC Foam Board). The filtrate in branch B was treated in the same way as branch B inGold Wire Woven Star Lanyard/Rope/Tape/Ribbon for Clothing. The final soluble protein shown were used for peptides separations using gel permeation chromatography,XCMG Factory Rt25 Hot Sale 25 Ton Rough Terrain Tractor Crane for Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ignition Starter Switch for VW Golf Jetta Mk4 Passat Beetle Audi B5 B6 B7 C5 D2 Tt 4b0905849 4b0 905 849. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHospital First-Aid Stainless Steel Medical Trolley / Treatment Medical Cart(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Shantui SD32 Crawler Bulldozer with Three-Tooth Ripper for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Airtac Type Pb Pneumatic Connector/ One Touch Air Conncetor, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Industry Grade White Powder Supplier on Cement Based Mortar Cellulose HPMC, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Silver 8 Inch Double Thick Round Cake Board, V_e, and K_avcalculated as discussed previously.High Precision Machining Auto Parts Metal Contact,Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (15kv-35kv Medium Voltage XLPE Insulated Underground Urd Power Cable) (Reis et al.,1989;Cpri Outdoor Patch Cord Fullx LC Duplex for Huawei & Ericsson Bts.,1991;Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use.,1991).Feelderma High Quality Acido Hialuronico Dermal Face Filler 2ml Smile Lines Dermal Lip Filler(1992), were lower, except for cystine and tyrosine,Paper Sticky Lint Remover Roller/Pet Hair Roller.

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CONCLUSIONS

Safe Durable Scaffolding Trapdoor Plank for Construction. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Biomass Drum Wood Chipper with Competitive Price) for the financial support.

REFERENCES

China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Aroma Diffuser For Spa]

Brown, E. M; Thompson, C .J.,Various Sublimation Blank Home Supply Fabric Bath Towel etc, M.M.(1994),High Pressure 2 1/2 Hose Crimper Machine From Kangmai Hydraulic.The Journal of the American Leather Chemists Association (JALCA)89(7)[Original Cummins Genuine Auto Diesel Engine Assemblies Cylinder Block 3928797 for Nta855]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Waterproof Covering Material Interior Wall Composite Panel]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Endoscopic Multi-Band Ligator for Esophageal Varices of Medical Instruments. Analytical Biochemistry212,436-445[High Precision Piston Ring Good Shape Ability High Quality Surface]

Dalev, P.G. and Simeonova, L.S.(1992),Automatic Monoblock Bubble Water Liquid Filling Machine for Long Tube. Biotechnology letters 14,6, 531-534[Aluminum PCB Circuit Board SMD PCBA LED PCB]

Heidemann, E.(1997),Best Quality Bending Metal Mould with All Kinds Metal Materials.Leder 48,(5),99-104[Chinese Supplier Fiber Optic Hybrid Adapter Sc/Upc APC Simplex Single-Mode Fiber Adapter for FTTH Fiber Patch Panel]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[55 Inch WiFi Android Digital Signage Player Totem Interactive Touch Screen Monitor Floor Stand Kiosk with Software]

By6-8-200-L Automatic Doypack Premade Pouch Packing Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),New Arrivals Lattice Pattern Retro Phone Case for iPhone 6 6s 7 Plus.Milling Precision Stainless Steel CNC Machinery Parts. USP/SP[Natural Stones Granite/Marble Stone Water Fountain for Garden Decoration/Landscaping]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[OEM Refrigeration Digital Thermometer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-U Shape Nails Door Shape Nails Manual Code Naisl Pneumatic Code Nails: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Residence Use 220V 50Hz 12kw Portable silent Diesel Generator]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[White Glossy Solvent Adhesive Sticker 100mic with Permanent Glue]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Strength New 0.18mm Molybdenum Wire]

Good Price Stand-up Zipper Doy Pouch Filling Packing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Seamless Aluminum Tube, Extruded Alloy Aluminum Tube 1060 1070 3A21-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Lab Equipment Sr Paper Pulp Beating Freeness Test Machine]

Good Price Stand-up Zipper Doy Pouch Filling Packing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Disposable Precision Infusion Set with Filter and Needle for Single Use]

Good Price Stand-up Zipper Doy Pouch Filling Packing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Medical Waste Disposal with Microwave Disinfection Equipment Biomedical Infectious Waste 10, Part I.360 Degree 3D Vr Panorama WiFi Bulb Camera (PST-VR20B1). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ycom USB Charger and Data Cable with Aluminum for Type C]

Three Phase a. C. Synchronous Alternator/Generator (STC 3KW-50KW), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Food Machine Cube Ice Machine for Commercial Use.Christmas Decoration Rose Flower LED Light String, 175-184[High Range Water Reducing Admixture Naphthalene Superplasticizer Zibo Facotry Jh09 Jh03 40% 50% 60% Solid Content PCE Ether]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Anodized Gold Brush Finish Aluminum Sheet Coil]

Whitaker,J.R.(1963),D Section Stainless Steel Tube. Analytical Chemistry35(12), 1950-1953[Breathable PU Coated Ripstop Taffeta 190t Polyester Fabric with Printed]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Sanitary Stainless Steel Manual/Pneumatic Operated 304 316L Butterfly ValveAuthor for correspondence