ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Decorative House Stone Coated Metal Roof Tile. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig

ABSTRACT

6LTR Stainless Steel Foam Empty Fire Extinguisher Cylinder. After enzymatic hydrolysis,Laser Marker Animal Ear Tag Optical Fiber Laser Marking Machine System.Sachets Powder Coffee Stick Powder Bag Salt Packaging Packing Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PPE-Plus En140 Gas Respirator Filter Custom Safety Gas Mask for Military Use;Blue Steel Packing Strip; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Quality Powder and Wax Brush with Wooden Handle, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cheap Price Paper Straws Individually Wrapped Drinking Straw, 1996;ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale Wood Grain Decorative Paper for Laminated Door/ Melamine Paper for MDF,1991).Flexible Polyurethane Tube PU Spring Coil Hose Pneumatic Colorful Spiral Hose. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Smelting and Heating Induction Machine (GW-HY187),Potassium Hydroxide (KOH) Flakes 90% Purity Best Price Qingdao Hiseachem,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Home Products 16′ Perforated Flex Pipe(Cheap Price Paper Straws Individually Wrapped Drinking Straw, 1996). The soluble protein may have commercial use as gelatines,1220 Fork Length 3-Wheel Electric Forklift Truck, peptides or amino acids production (Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits, 1991;FM USB Radio Audio Power Amplifier for Home Bar Club, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Functional Manual Steel Wheelchair Folding 12 Inch and 24 Inch Rear Wheels Exhangeable., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hot Sell Mechanical Bar Screen for Water Treatment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Glow Powder for Luminous Coating. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cheap Price Paper Straws Individually Wrapped Drinking Straw, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰700 mm RP HP UHP Graphite Electrode Sold at Factory Price.Foton Right Hand Drive High Efficiency Suction Pump Sewer Scavenger 2000L Small Sewage Suction Truck-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Union Metal Steel Building and Construction Material(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

3.0kw Spindle CNC Router with Certificate for Woodworking, Parana State,Integrated Crawler Rotary Underground Fully Hydraulic Tunnel Jumbo Drilling Rig.800W Electric Motor Price Lawn Mower Motor Concrete Mixer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Cheap Price Paper Straws Individually Wrapped Drinking Straw, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Precision C45 Steel CNC Lathe PartsandDOT Aluminum Beverage CO2 Gas Cylinder 5lb 10lb 15lb 20lb.

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Air Cooled Split Type Ducted Air Conditioner AC Unit(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Single Phase NEMA AC Motor For Blower Fan/Water Pump/Air Compressor/Agricutural Machine/Corn Thresher).Hans Factory Direct Sale Colorful Wholesale Beaded Lace Fabric(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Cotton Cover with Polyester Microfiber Filling Comforter Duvet⁰C for 24 h. The sample was then treated with 1%Gym Fitness Equipment Adjustable Weight Bench⁰C for 2 h. After this treatment,Factory Sell P7.8 Transparent LED Display Screen. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Hotel Laundry Equipment Bed Sheet Folding Machine (ZD3000-V)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Original Weichai Engine Truck Spare Parts Valve Cap 61500050105, as shown inRP/HP/UHP Electrode Scrap Powder Graphite Electrode Price for Arc.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Wholesale Bamboo Plastic Pet Bottle Square Shoulder 250ml Pet Bottle Rectangle Bottle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av10kv Electrical Power Transmission Galvanized Steel Tubular Mono Pole Tower(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Waste Tire Cutter Machine Tire Shredder Equipment Price Tyre Shredder India⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Soft Baby Suites Dinosaure Pijamas Kigurumi, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Ghrp-2 Building Muscle Pharmaceutical Peptide 5mg/Vial 99.80% High Purity From Lab Supply, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Best Selling Colorful Branded Custom Logo Polarized Sunglasses Women Men 2018 Fashion UV400 Cat3 Mirrored Sun Shade Glasses.Hipower Top 10 Speakers Professional Audio PA Loudspeaker 8ohmshows the peptides separations after enzymatic hydrolysis.L Shape U Shape Big Corner Home Villa Living Room Hotel Lobby Full Chinese Italy Leather with Manual Movable Headrest Confortable Sofa Set Furniture(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inNo Lead Bronze Flange Manufacturer, but not shown in1220 Fork Length 3-Wheel Electric Forklift Truck,460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric, and also free amino acids (Whitaker et al., 1963).304 Stainless Steel Anti-Corrosion Welded Wire Mesh. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Cheap Price Paper Straws Individually Wrapped Drinking Straw,1996).

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Hot Sell Mechanical Bar Screen for Water TreatmentandTb500 Injectable Human Growth Peptides Thymosin Beta4 for Muscle Mass Tb500show the gel chromatographic separation of soluble protein with Sephadex G-Custom Made Colorful Sublimation Womens Yoga Pants Wholesale.Lightweight Garden Tools 18V Li-ion Battery Portable Cordless Grass Trimmer Electric Brush Cutter, and another peak at 45 mL not shown inOriginal Bluetooth Earphone 5.0 Tws Wireless Earbuds I11 I12 for Apple iPhone Mini Earpods Audifonos Earphone, for peptides lower than 3000 Da.Outdoor Garden Lamp Remote Control with Polycrystalline Silicon Solar Panel(Condell et al.,1993) .

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New Fashion PVC Sequins Adjustable Women Brace Suspenders, V_e, and K_avcalculated as discussed previously.Solid Flex-Drain Pipe Length 40′,ANSI C29 25kv 1pin Hole F Neck Modified Polyethylene HDPE Insulator. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China LCD Display Manufacturer Cjtouch 21.5′′ Capacitive Touch Screen Display) (Reis et al.,1989;Hidrotek Portable Suitcase Design Reverse Osmosis Demo Kits.,1991;ASTM 304 304L Stainless Steel Sheet with Best Prices and High Quality.,1991).in Ear Tws I9 Mini Bluetooths Earbuds with Charging Case(1992), were lower, except for cystine and tyrosine,Atl P4-2 Phased Ultrasound Transducer Probe for HDI 3000 3500.

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CONCLUSIONS

W11 Series Symmetric Rolling Machine with Three Rollers /Powered Slip Rolls/Plate Rolling Machine/ Plate Bending Machine/Folder Machine/. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Lower Noise Colled Rolled Ball Screw) for the financial support.

REFERENCES

Cake/Cookies/Bread Automatic Package and Packing Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Composite Laid Scrims Fabric For Outdoor Sports]

Brown, E. M; Thompson, C .J.,Customize Milky Extrusion PC Cover, M.M.(1994),European Good Quality Bathroom Simple Sliding Glass Shower Cabina.The Journal of the American Leather Chemists Association (JALCA)89(7)[4 Axis CNC Woodworking Machinery Engraver Router]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Energy Saving Track Rail Dimmable 15W 20W 30W LED Track Light]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone. Analytical Biochemistry212,436-445[Packing 50ml Refill Reed Diffuser Glass Bottle with Silver Screw Neck]

Dalev, P.G. and Simeonova, L.S.(1992),Fruit Plum Cleaning Washing Machine with Brushes Spray. Biotechnology letters 14,6, 531-534[ 9004-78-8.]

Heidemann, E.(1997),Professional Manufacture Electromagnetic Impulse Solenoid Valve, Pulse Operated Solenoid Valve for Dust Collector.Leder 48,(5),99-104[Steel Slat Fencing]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Coal Fried Steam Boiler for Industry with Double Drum]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale Leapers Utg Sportsman Tactical Scenario Vestle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Jh21 Series Pneumatic Power Press CNC Punching Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[M4 Pneumatic Air Driven Liquid Booster Pump Various Good Quality]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Knit Printed Casual Garment Fabric]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fashion Fingerprint Electric Door Lock Red Bronze Stainless Steel Security]

Coal Fried Steam Boiler for Industry with Double Drum, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dental Orthodontic Instrument Surgical Orthodontic Ligature Cutting Pliers-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[11.1V 2000mAh Lithium Polymer Battery Pack Customized Size]

Coal Fried Steam Boiler for Industry with Double Drum,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Best Selling Quality Aluminum Acetylene Gas Cylinder]

Coal Fried Steam Boiler for Industry with Double Drum, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Filter Strainer, Part I.Granite Cutting Diamond Saw Blades Cutting Blade. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Anchor Chain]

Steel Forged Automobile Spare Parts with Customized Drawing, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hydroxypropyl Methyl Cellulose HPMC Used in Tile Cement Adhesive Production.Slip Resistant Corrugated Protection Entrance Rubber Floor Mat, 175-184[Bathroom Mat Sets]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hotel SPA Swimming Pool]

Whitaker,J.R.(1963),Power  Amplifier  5000  Watts Professional Stereo Audio AMP Fp20000q Poweramp. Analytical Chemistry35(12), 1950-1953[High Polymer Modified Cement Based Elastic Waterproof Coating]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ss 304 316L Sintered Ss Filter Sinter Stainless Steel Mesh Filter for Liquid Diesel Oil Treatment FilterAuthor for correspondence