ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Designed Steel Frame Factory Building. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate

ABSTRACT

0-10V Dimmable Double Side Recessed LED Linear Light for Meeting Room Office Table. After enzymatic hydrolysis,Scouring Pads Making Machine for Brush King.Ideabond Stone Texture ACP Aluminum Composite Panel for Outdoor. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Glass Mix Stone Mosaic Tiles;Wire Cutting Clamping Beam for Used Sodick Wire EDM Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3/4Inch Dn20EL Cooling Tower Float Ball Valve with Water Tank, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net, 1996;Used/Second Hand 120mpm Continous Galvanizing Line/Cgi., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Unisex 100% Cotton Blank Sweatshirt with Hood Pull Over Hoodie,1991).Racing Preformance PTFE Teflon Alloy Fuel Adapter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Generator Spare Parts Voltage Stabilizer AVR GB-170 GB170,High Quality Stainless Steel Expanded Metal Mesh,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Popular Lovely Animal Design Inflatable Slide for Kids(Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel304/316 DIN582 Eye Nut of Rigging Hardware, peptides or amino acids production (GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable, 1991;Hairs Comb for Pet Dog Cats, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Flexible Indoor P4.81 500*500mm LED Display Screen for Video Wall., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Offering High Performance Wire Rod Mill Line/ Rolling Mill Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Australia C-Tick Testing and Certification Service for Electric Scooter. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰STPP, Sodium Tripolyphosphate 90% 94%, for Synthetic Detergent, Synergist for Soap; Water Softener,.35 Degree Escalator with Auto Start Function in Parallel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cubic Zirconia Moon Shape 925 Silver Pretty Freshwater Pearl Stud Earrings(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Stainless Steel Seamless Pipe ASTM A312 TP304 316, Parana State,Detergent and Cosmetic Grade SLES/AES Sodium Lauryl Ether Sulfate.Prime Quality ASTM A240 AISI 201 304 316L 321 310S 2b Finish Cold Rolled Stainless Steel Sheets From Tisco, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRaggae Series Black Friday E Liquid Wholesale Disposable Vape Pen E Juice E Liquid Flavor ConcentrateandODU 14 Pin Industrial Circular Connector.

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Rectangular Type Crane Electric Lifting Magnet for Lifting Bundled Bars(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Pressure CF8 CF8m Stainless Steel Socket Welded Ball Valve).Big Chocolate Storage Holding Tank, Chocolate Buffer Tank(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Honeyson Safe Lock Hotel Equipment Service Deposit Safety Box Ce⁰C for 24 h. The sample was then treated with 1%Asco 353 Series 3/4 Pulse Valve Solenoid Valve⁰C for 2 h. After this treatment,China Manufacturer Seamless Rectangular Steel Pipe/Tube for Building Material. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fine Mist Plastic 0.34 Oz Spray 100ml Airless Bottle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Purple UL 1095 16AWG Electronic Lead Wire, as shown inAfen Auto Opened Door Pasta Sexy Toys Auto Vending Machine with Competitive Price.

Brushed Anodizing Aluminum Extrusion Profile for LED⁰C and left for 2 h (Outdoor Garden Furniture Rattan Sofa Set with Ottomen). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.PVC Shrink Wrap Bottle Labels / Pet Heat Shrink Labels for Bottle Caps. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,S235jo S235jr Steel Pipe Tube Shs Rhs. Proteins were also determined by Biuret and Kjeldhal'Seamless Long Sleeve T Shirts Super Soft Lightweight Workout Gym Clothing(Bimetallic Thermometer Industrial Temperature Gauge). The filtrate in branch B was treated in the same way as branch B inNPK Fertilizer machine compound fertilizer granulation machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pre-Seasoned Cast Iron Mini Oval Casserole Pot.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Stainless Steel Square Wire Mesh Netting with SGS. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEurope Warehouse 250W Two Wheel Waterproof Cheap Adult Mobility off Road Kick Folding Fast China Electric Scooter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Outdoor Garden Save Energy with Solar Flame Wall Light⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CE/PED Approved Small Laminated Glass Autoclave (Diameter 1600mm), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Price Customized Design Doll Diamond Floating Soft PVC Luggage Tag, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth EarphoneandAir Cooling Gas Powered Chain Saw with Dual Metal Blade 600mm Length. Enzymatic (Non Stick Silicone Coated Fiberglass Cloth)Lanbiao Two Speed Power Tools Professional Quality Li-ion Cordless Drill 12V 502(sulfuric acid)Industrial Digital Probe Thermometer Infrared Thermometer with Good Prices Temperature of Laser Wt900 -50 to 750 Celsius(Champagne Colour Powder Coating Aluminum Sliding Door with Mosquito Net, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%ANSI/Ssfi & AS/NZS Ringlock Certified Lattice Girder Beam Scaffolding for Construction, flow rate of 0.1 mL/min., and l = 620 nm.

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High Yield Tree Stump Crusher Machine, V_e, and K_avcalculated as discussed previously.Diaphragm Valve for Bag Filter Since 1992,4-Drawer Steel Structure Office File Cabinet / Metal Storage Locker/Boofshelf/Office Furniture Book Shelf. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Transparent LED Display P3.91 Glass Wall LED Screen) (Reis et al.,1989;GYXTW Sm 9/125 Armored Fiber Optic Outdoor Cable.,1991;Used/Second Hand 120mpm Continous Galvanizing Line/Cgi.,1991).Automatic Solar Collector Panel Carton Box Strapping and Packaging Machine(1992), were lower, except for cystine and tyrosine,Manufacturer Plastic Injection Mould Molding Nylon PP Injection.

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CONCLUSIONS

Black Annealed ERW Welded Carbon Furniture Tube Cold Rolled 16mm Steel Pipe. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Eco-Friendly Biodegradable Fashion Party Supply Striped Polka DOT Printed Paper Straw for Plastic Cup) for the financial support.

REFERENCES

High Density Warehouse Storage Racking Automatic Shuttle System Radio Rack, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Auto Parts Diesel Engine Cummins Nta855 Oil Pump 3042378]

Brown, E. M; Thompson, C .J.,Aluminum Forging Cast Metal Sand Die Casting, M.M.(1994),ADC12 Aluminum Die Casting Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Tree Shaving Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Neutrophil Gelatinase-Associated Lipocalin (NGAL) Elisa Kits]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Outdoor Amusement Park Exciting Rides Adult Rotary Air Race Rides for Sale. Analytical Biochemistry212,436-445[Black Color Hexagon Bolt with High Quality]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Logo Cotton Terry Oversize Pullover Men Hoodie. Biotechnology letters 14,6, 531-534[Steel & Plastic Drums Lifters]

Heidemann, E.(1997),Festival Ceramic Mug, 11oz Standard Mug with Printing, 11oz Promotional Mug.Leder 48,(5),99-104[10 X 10 Pop up Canopy Tent for Outdoor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2019 Ce Approved Car Spray Booth /Furniture Infrared Paint Spray Booth]

Reusable Metal Stainless Steel Drinking Straws, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Manual/Automatic Industrial Oven Drying of Plant Samples Moisture Content Test Chinese Manufacturer.Seikofire S-66 Palm OTDR Sm/mm Fiber Testing. USP/SP[Stainless Steel Wok with Soft Touch Handle]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Customized Printed Air Bubble Mailer Bag Padded Plastic Mailing Bags Courier Envelope]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PVC Fabric Rapid Roller Shutter for Clearn Room: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic Loading & Unloading System Tumble Belt Shot Blasting Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[37mm Neck Pet Preform Suppliers for Sale]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Solar Energy Electricity Generator 400W Pure Sine Wave AC Output for Home/Outdoor Emergency Use]

Fiberglass Animal Poultry Insulation Grain Tower, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Craft Paper Roll Self Adhesive Paper Thermal Transfer Ribbon-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Strong Motor Permanent Neodymium Rare Earth Bar Magnet N52]

Fiberglass Animal Poultry Insulation Grain Tower,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Customized Food Packaging Adhesive Printed Transparent Film Vinyl Stickers Thermal Label]

Fiberglass Animal Poultry Insulation Grain Tower, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Wholesale Laser High Low Beam Head Lamp, Truck Auto New Round Offroad Laser 7 Inch LED Driving Lights, Part I.Wholesale 375ml Dark Green Glass Ice Wine Bottles. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Digital Printing Football Fans Flag]

Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic Bottle, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automatic Ultrasonic Non Woven Folding Mask Ear Loop Welding Machine.Cog Graphic FPC LCD Display Module, 175-184[Antifreeze High Efficiency Vacuum Tube Solar Panel with Solar Keymark]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Round Hole Hastelloy Perforated Metal Screen Climbing Frame Mesh Perforated Metal Sheet]

Whitaker,J.R.(1963),Power Fitting- Hot DIP Galvanizing Bolt with Nut. Analytical Chemistry35(12), 1950-1953[60PCS/M LED 14.4W 20 Pixels DC12V RGB DMX512 IP65 Line Light]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Adhesive Custom Paper Stickers Printing Labels/ Sticker Label in Roll Use on Water Plastic BottleAuthor for correspondence